公开(公告)号：WO2018098214A1

公开(公告)日：2018-05-31

申请号：PCT/US2017/062923

申请日：2017-11-22

Applicant: BAYER CROPSCIENCE LP

Inventor： DOROGHAZI, James ,  LEHTINEN, Duane ,  RODGERS-VIEIRA, Elyse ,  BAEK, Jongmin

IPC: C07K14/325 ,  C12N15/82

Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, or the nucleotide sequence set forth in SEQ ID NO:1, as well as variants and fragments thereof.

公开(公告)号：WO2017066479A1

公开(公告)日：2017-04-20

申请号：PCT/US2016/056898

申请日：2016-10-13

Applicant: BAYER CROPSCIENCE LP ,  BAYER CROPSCIENCE AG

Inventor： RODGERS-VIEIRA, Elyse ,  SAMPSON, Kimberly ,  LEHTINEN, Duane ,  LOESEL, Peter ,  PORTZ, Daniela

IPC: C07K4/04 ,  C12N15/82 ,  C07K14/21

CPC classification number: C12N15/8286 ,  C07K14/21 ,  Y02A40/162

Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:4-l l, or the nucleotide sequence set forth in SEQ ID NO: 1-3, as well as variants and fragments thereof.

Abstract translation: 提供了赋予细菌，植物，植物细胞，组织和种子杀虫活性的组合物和方法。 提供了包含毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中用于在植物和细菌中转化和表达。 组合物还包含转化的细菌，植物，植物细胞，组织和种子。 具体而言，提供了分离的毒素核酸分子。 此外，还包括对应于多核苷酸的氨基酸序列，以及与那些氨基酸序列特异性结合的抗体。 具体而言，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：4-11所示氨基酸序列或SEQ ID NO：1-3所示核苷酸序列的核苷酸序列，以及变体和 片段。