公开(公告)号：WO2021222927A1

公开(公告)日：2021-11-04

申请号：PCT/US2021/070459

申请日：2021-04-27

申请人： BAYLOR COLLEGE OF MEDICINE

发明人： QUACH, David H. ,  ROONEY, Cliona M. ,  RAMOS, Carlos A.

IPC分类号： A61K35/17 ,  A61P35/00 ,  C07K14/725 ,  C07K16/28 ,  C07K14/705

摘要： Virus-specific immune cells, comprising a chimeric antigen receptor (CAR) or nucleic acid encoding a CAR, wherein the CAR comprises: (i) an antigen-binding domain which binds specifically to CD30, (ii) a transmembrane domain, and (iii) a signalling domain, wherein the signalling domain comprises: (a) an amino acid sequence derived from the intracellular domain of CD28, and (b) an amino acid sequence comprising an immunoreceptor tyrosine-based activation motif (ITAM), are disclosed. Also disclosed are methods for producing and compositions comprising such cells.

公开(公告)号：WO2022232797A2

公开(公告)日：2022-11-03

申请号：PCT/US2022/071950

申请日：2022-04-27

申请人： BAYLOR COLLEGE OF MEDICINE

发明人： QUACH, David H. ,  ROONEY, Cliona M. ,  RAMOS, Carlos A.

IPC分类号： C12N5/0783 ,  A61K35/17 ,  A61K39/245 ,  C07K14/05 ,  A61P35/02 ,  A61K2039/5156 ,  A61K39/001112 ,  A61K39/001129 ,  C07K14/005 ,  C12N2501/2302 ,  C12N2501/2307 ,  C12N2501/2315 ,  C12N2501/51 ,  C12N2501/515 ,  C12N2501/998 ,  C12N2506/11 ,  C12N2510/00 ,  C12N2710/16234 ,  C12N5/0636

摘要： Embodiments of the disclosure encompass methods for generating or expanding a population of immune cells specific for a virus, comprising stimulating immune cells specific for a virus by culturing peripheral blood mononuclear cells (PBMCs) in cell culture medium comprising human platelet lysate in the presence of: (i) one or more peptides corresponding to all or part of one or more antigens of the virus; or (ii) antigen presenting cells (APCs) presenting one or more peptides corresponding to all or part of one or more antigens of the virus. In particular embodiments, the cell culture medium comprises a particular percentage of human platelet lysate and/or the PBMCs are depleted of CD45RA-positive cells, for example.

公开(公告)号：WO2021222929A1

公开(公告)日：2021-11-04

申请号：PCT/US2021/070461

申请日：2021-04-27

申请人： BAYLOR COLLEGE OF MEDICINE

发明人： QUACH, David H. ,  ROONEY, Cliona M. ,  RAMOS, Carlos A.

IPC分类号： A61K35/17 ,  A61P35/00 ,  C07K14/725 ,  C07K16/28 ,  C07K14/705

摘要： Treatment and prevention of diseases/conditions characterised by an alloreactive immune response using virus-specific immune cells, comprising a chimeric antigen receptor (CAR) or nucleic acid encoding a CAR, wherein the CAR comprises: (i) an antigen-binding domain which binds specifically to CD30, (ii) a transmembrane domain, and (iii) a signalling domain, wherein the signalling domain comprises: (a) an amino acid sequence derived from the intracellular domain of CD28, and (b) an amino acid sequence comprising an immunoreceptor tyrosine-based activation motif (ITAM), is disclosed.

公开(公告)号：WO2021222928A1

公开(公告)日：2021-11-04

申请号：PCT/US2021/070460

申请日：2021-04-27

申请人： BAYLOR COLLEGE OF MEDICINE

发明人： QUACH, David H. ,  ROONEY, Cliona M. ,  RAMOS, Carlos A.

IPC分类号： A61K35/17 ,  A61P35/00 ,  C07K14/725 ,  C07K16/28 ,  C07K14/705

摘要： Treatment and prevention of cancer using virus-specific immune cells, comprising a chimeric antigen receptor (CAR) or nucleic acid encoding a CAR, wherein the CAR comprises: (i) an antigen-binding domain which binds specifically to CD30, (ii) a transmembrane domain, and (iii) a signalling domain, wherein the signalling domain comprises: (a) an amino acid sequence derived from the intracellular domain of CD28, and (b) an amino acid sequence comprising an immunoreceptor tyrosine-based activation motif (ITAM), is disclosed.