公开(公告)号：WO2015097061A1

公开(公告)日：2015-07-02

申请号：PCT/EP2014/078470

申请日：2014-12-18

Applicant: BRUKER DALTONIK GMBH

Inventor： BIERBAUM, Gabriele ,  SZEKAT, Christiane ,  ALSABTI, Nahed ,  JOSTEN, Michaele ,  REIF, Marion

IPC: G01N33/68

CPC classification number: C12Q1/14 ,  G01N33/6851 ,  G01N2333/31 ,  G01N2800/44

Abstract: The invention provides a method for identifying methicillin resistant Staphylococcus aureus (MRSA) in a bacterial sample comprising the steps: classifying bacteria in the sample as Staphylococcus aureus (SA) and determining the presence or absence of the phenol soluble modulin peptide or a variant thereof wherein the presence of the PSM-mec peptide or variant thereof indicates methicillin resistant Staphylococcus aureus. The variant is preferably the formylated version of the PSM-mec peptide having a mass to charge ratio of 2415 in a singly protonated state.

Abstract translation: 本发明提供了一种在细菌样品中鉴定耐甲氧西林金黄色葡萄球菌（MRSA）的方法，包括以下步骤：将样品中的细菌分类为金黄色葡萄球菌（SA），并测定苯酚可溶性调节素肽或其变体的存在或不存在，其中 PSM-mec肽或其变体的存在表示耐甲氧西林金黄色葡萄球菌。 该变体优选是在单质子化状态下质量与电荷比为2415的PSM-mec肽的甲酰化形式。