公开(公告)号：WO2017015652A1

公开(公告)日：2017-01-26

申请号：PCT/US2016/043815

申请日：2016-07-25

Applicant: ENBIOTIX, INC.

Inventor： FERULLO, Daniel, J. ,  RADDING, Jeffrey, A.

IPC: A61K35/76 ,  A61K38/16 ,  A61K39/085 ,  C12N7/01 ,  C12Q1/70 ,  C12Q1/14 ,  A61P31/04

CPC classification number: A61K35/76 ,  A61K38/10 ,  A61K38/1729 ,  A61K38/1767 ,  A61K38/47 ,  A61K38/48 ,  C12N7/00 ,  C12N2795/10221 ,  C12N2795/10232 ,  C12Q1/14 ,  C12Q1/689 ,  C12Y302/01017 ,  C12Y302/01052 ,  C12Y304/24075 ,  G01N2333/31

Abstract: The present invention relates to GRCS bacteriophages, as well as to methods and compositions for the treatment of prosthetic joint infection. Particularly, the present invention provides bacteriophages specific against Staphylococcus aureus from prosthetic joint infections.

Abstract translation: 本发明涉及GRCS噬菌体，以及用于治疗假体关节感染的方法和组合物。 特别地，本发明提供了针对来自假体关节感染的针对金黄色葡萄球菌的噬菌体。

公开(公告)号：WO2015184270A1

公开(公告)日：2015-12-03

申请号：PCT/US2015/033203

申请日：2015-05-29

Applicant: LI, Xiujun ,  DOU, Maowei

Inventor： LI, Xiujun ,  DOU, Maowei

IPC: G01N33/53

CPC classification number: G01N33/54386 ,  B01L3/5023 ,  B01L3/5027 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0681 ,  B01L2300/069 ,  B01L2300/0803 ,  B01L2300/0864 ,  B01L2300/126 ,  B01L2400/0409 ,  C12Q1/689 ,  G01N33/56911 ,  G01N2333/22 ,  G01N2333/255 ,  G01N2333/31 ,  G01N2333/3156 ,  G01N2333/335

Abstract: Certain embodiments are directed to paper/polymer hybrid microfluidic devices integrated with nano-biosensors for pathogen detection and infectious disease diagnosis. Microfluidics provides for minimal reagent consumption, integrated processing, and analysis of complex biological fluids with high efficiency and sensitivity. The devices and methods described herein provide for simple, rapid, and sensitive pathogen detection at low cost. Embodiments include methods and devices for direct pathogen detection.

Abstract translation: 某些实施方案涉及与用于病原体检测和感染性疾病诊断的纳米生物传感器集成的纸/聚合物杂交微流体装置。 微流体提供了最低的试剂消耗，综合处理和复杂生物流体的分析，具有高效率和灵敏度。 本文所述的装置和方法以低成本提供简单，快速和敏感的病原体检测。 实施方案包括用于直接病原体检测的方法和装置。

公开(公告)号：WO2014073858A1

公开(公告)日：2014-05-15

申请号：PCT/KR2013/010025

申请日：2013-11-06

Applicant: 주식회사 현일바이오

Inventor： 김정욱

IPC: C12Q1/68 ,  C12N15/11 ,  C12Q1/04

CPC classification number: C12Q1/689 ,  C12Q1/04 ,  C12Q2600/16 ,  G01N2333/31 ,  G01N2800/44

Abstract: 본 발명은 다양한 프라이머 및 프로브를 이용한 3개의 타겟 유전자를 동시에 증폭하여 분석하여 MRSA를 검출하는 방법 및 이를 이용한 진단키트에 관한 것이다. 본 발명은 타겟 유전자(바람직하게는, mecA, SCCmec/orfX 및 16S rRNA)-특이적인 프라이머와 프로브를 이용한 멀티플렉스 실-시간 PCR(real-time polymerase chain reaction)을 통해 MRSA를 효과적으로 검출하고 이를 다른 스트레인들과 용이하게 구별할 수 있다. 또한, 본 발명의 진단키트는 시료 내 타겟 유전자들을 멀티플렉스 실-시간 PCR을 통해 간편하고 효율적으로 검출할 수 있다. 따라서, 본 발명의 방법 및 키트는 MRSA의 정확한 진단 뿐 아니라 감염을 검출할 수 있으며, 이를 기반으로 보다 정확하게 질환의 예후를 예측하고 치료에 적용될 수 있다.

Abstract translation: 本发明涉及通过使用各种引物和探针同时扩增和分析三种靶基因来检测MRSA的方法，以及使用其的诊断试剂盒。 本发明可以有效地检测MRSA并且通过使用靶基因（优选mecA，SCCmec / orfX和16S rRNA）特异性引物和探针通过多重实时聚合酶链反应（PCR）容易地将其与其它菌株区分开。 此外，本发明的诊断试剂盒可以通过多重实时PCR方便有效地检测样品中的靶基因。 因此，本发明的方法和试剂盒可以检测MRSA感染并精确诊断MRSA，更准确地预测疾病的预后并根据结果治疗疾病。

公开(公告)号：WO2014013272A1

公开(公告)日：2014-01-23

申请号：PCT/GB2013/051947

申请日：2013-07-19

Applicant: KINGSTON UNIVERSITY HIGHER EDUCATION CORPORATION

Inventor： SINCLAIR, Alex ,  FIELDER, Mark ,  LE GRESLEY, Adam

IPC: C07K5/083 ,  A61K49/00 ,  C12Q1/37 ,  G01N33/58 ,  C09B11/24 ,  C07D493/10

CPC classification number: C12Q1/14 ,  A61K49/0017 ,  C07D493/10 ,  C07K5/0808 ,  C07K5/0817 ,  C09B11/24 ,  C12Q1/37 ,  G01N33/542 ,  G01N2333/31 ,  G01N2333/952

Abstract: The present invention concerns rhodamine based fluorescent probes which have use in detecting coagulase-producing bacterial strains. In particular, wherein the bacterial strain is MRSA or MSSA.

Abstract translation: 本发明涉及用于检测产生凝固酶的细菌菌株中的罗丹明的荧光探针。 特别地，其中细菌菌株是MRSA或MSSA。

公开(公告)号：WO2013130875A1

公开(公告)日：2013-09-06

申请号：PCT/US2013/028409

申请日：2013-02-28

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： SUPER, Michael ,  INGBER, Donald E. ,  CARTWRIGHT, Mark J. ,  WATTERS, Alexander ,  WORKMAN, Sam ,  LEVNER, Daniel ,  ROTTMAN, Martin

IPC: C12Q1/18 ,  C12Q1/04 ,  C12Q1/02

CPC classification number: G01N33/56938 ,  C12Q1/18 ,  G01N33/56911 ,  G01N33/56916 ,  G01N2333/245 ,  G01N2333/31 ,  G01N2500/10

Abstract: Embodiments of various aspects described herein are directed to methods, compositions, kits and systems for rapid determination of antibiotic susceptibility of a microbe within hours after a sample is collected. In some embodiments, the methods, compositions, kits and systems described herein can allow determination of antibiotic susceptibility of a microbe based on a small number of microbes, e.g., as few as 5-10 microbes bound to a microbe-targeting substrate described herein.

Abstract translation: 本文描述的各个方面的实施方案涉及用于在收集样品后几小时内快速测定微生物的抗生素敏感性的方法，组合物，试剂盒和系统。 在一些实施方案中，本文所述的方法，组合物，试剂盒和系统可以允许基于少量微生物测定微生物的抗生素敏感性，例如与本文所述的微生物靶向底物结合的少至5-10微克。

公开(公告)号：WO2012126882A1

公开(公告)日：2012-09-27

申请号：PCT/EP2012/054803

申请日：2012-03-19

Applicant: MIACOM DIAGNOSTICS GMBH ,  STEIN, FREIHERR VON, Walter ,  STANGE, Mirko

Inventor： STEIN, FREIHERR VON, Walter ,  STANGE, Mirko

IPC: C12Q1/18 ,  G01N33/569 ,  C12Q1/68 ,  C12N15/82

CPC classification number: C12Q1/689 ,  C07K16/40 ,  C12Q2600/106 ,  C12Q2600/136 ,  G01N33/569 ,  G01N2333/31 ,  G01N2800/44

Abstract: The invention relates to a method and a kit for the detection of an antibiotic resistance in a predetermined micro-organism in a biological sample. The method according to the invention comprises the following steps: (a) contacting the biological sample with a first nucleic acid labelled with a first label and capable of selectively hybridizing with a nucleic acid in the micro-organism, (b) identifying the micro-organism by the detection of the presence of the first label in an individual cell of the micro-organism, (c) contacting the sample with at least one probe for detection of an antibiotic resistance in a micro-organism, wherein said at least one probe is labelled with at least a second label, and (d) determining the antibiotic resistance of the micro-organism by the detection of the presence of at least the second label, wherein steps (b) and (d) are performed simultaneously so as to allow quick identification of a pathogen directly from a sample without culturing and without prior amplification.

Abstract translation: 本发明涉及用于检测生物样品中预定微生物中的抗生素抗性的方法和试剂盒。 根据本发明的方法包括以下步骤：（a）使生物样品与标记有第一标记并能够与微生物中的核酸选择性杂交的第一核酸接触，（b）鉴定微生物， 通过检测微生物的单个细胞中第一标记的存在，（c）使样品与至少一个用于检测微生物中的抗生素抗性的探针接触，其中所述至少一个探针 标记有至少第二标记，和（d）通过检测至少第二标记的存在来确定微生物的抗生素抗性，其中步骤（b）和（d）同时进行，以便 允许直接从样品中快速鉴定病原体而不进行培养，无需先前的扩增。

公开(公告)号：WO2010099068A1

公开(公告)日：2010-09-02

申请号：PCT/US2010/024932

申请日：2010-02-22

Applicant: 3M INNOVATIVE PROPERTIES COMPANY ,  MACH, Patrick, A. ,  ROSAUER, Michelle, L. ,  HUGHES, Michael, E.

Inventor： MACH, Patrick, A. ,  ROSAUER, Michelle, L. ,  HUGHES, Michael, E.

IPC: C12Q1/04 ,  C12Q1/14

CPC classification number: C12Q1/34 ,  C12Q1/04 ,  G01N2333/31 ,  G01N2333/922

Abstract: The disclosure provides articles and methods useful for detecting a discrete source of DNase activity. DNase-producing microorganisms can be detected. The device can further include selective agents and/or indicators to differentiate groups or species microorganisms. Methods of use include detecting or enumerating DNase- producing microorganisms.

Abstract translation: 本公开提供了用于检测离散源DNA酶活性的文章和方法。 可以检测产生DNA酶的微生物。 该装置可以进一步包括用于区分组或物种微生物的选择剂和/或指示剂。 使用的方法包括检测或列举产生DNase的微生物。

公开(公告)号：WO2010036795A3

公开(公告)日：2010-07-01

申请号：PCT/US2009058208

申请日：2009-09-24

Applicant: TRANA DISCOVERY INC ,  GUENTHER RICHARD H ,  YENNE SAMUEL P

Inventor： GUENTHER RICHARD H ,  YENNE SAMUEL P

IPC: C12N15/11 ,  C07H21/00 ,  C12Q1/68 ,  C12R1/445 ,  G01N33/15

CPC classification number: C12N15/11 ,  C12Q1/18 ,  C12Q1/6806 ,  C12Q1/6895 ,  C12Q2600/136 ,  C12Q2600/158 ,  G01N2333/31 ,  Y10T436/143333 ,  C12Q2525/117 ,  C12Q2563/107

Abstract: Methods of inhibiting S. aureus propagation, and screening for compounds that inhibit S. aureus propagation, are described. A method of inhibiting S. aureus propagation comprises inhibiting ribosomal binding of a specific S. aureus tRNA in the S. aureus by an amount sufficient to inhibit S. aureus protein expression. A method of screening for compounds useful for inhibiting S. aureus propagation comprises contacting a specific S. aureus tRNA to a ribosome that binds that tRNA in the presence of the test compound, and then determining whether the compound inhibits the binding of that tRNA.

Abstract translation: 描述了抑制金黄色葡萄球菌繁殖的方法和筛选抑制金黄色葡萄球菌繁殖的化合物。 抑制金黄色葡萄球菌繁殖的方法包括以足以抑制金黄色葡萄球菌蛋白质表达的量抑制金黄色葡萄球菌中特异性金黄色葡萄球菌tRNA的核糖体结合。 筛选用于抑制金黄色葡萄球菌繁殖的化合物的方法包括使特异性金黄色葡萄球菌tRNA与在测试化合物存在下结合该tRNA的核糖体接触，然后确定该化合物是否抑制该tRNA的结合。

公开(公告)号：WO2010069335A2

公开(公告)日：2010-06-24

申请号：PCT/DK2009/050348

申请日：2009-12-18

Applicant: KØBENHAVNS UNIVERSITET ,  PETERSEN, Morten Rønn ,  BOJESEN, Anders Miki

Inventor： PETERSEN, Morten Rønn ,  BOJESEN, Anders Miki

IPC: G01N33/569

CPC classification number: G01N33/56911 ,  G01N2333/21 ,  G01N2333/245 ,  G01N2333/295 ,  G01N2333/31 ,  G01N2333/315 ,  G01N2800/364

Abstract: The present invention relates to methods for diagnosing and treatment of a dormant infection of at least one pathogen. The invention further relates to a composition comprising an anti-dormancy factor as well as to said composition for use in a method of diagnosis or treatment and also to a method for manufacturing said composition. The invention further relates to a kit of parts comprising, inter alia, said composition. The invention also relates to a method for activating a dormant infection.

Abstract translation: 本发明涉及用于诊断和治疗至少一种病原体的休眠感染的方法。 本发明进一步涉及包含抗休眠因子的组合物以及用于诊断或治疗方法的所述组合物，还涉及用于制造所述组合物的方法。 本发明还涉及包含所述组合物的部件套件。 本发明还涉及激活休眠感染的方法。

公开(公告)号：WO2010060216A1

公开(公告)日：2010-06-03

申请号：PCT/CA2009/001728

申请日：2009-11-26

Applicant: NATIONAL RESEARCH COUNCIL OF CANADA ,  NATIONAL CHUNG CHENG UNIVERSITY ,  HUANG, Ping-Ji ,  CHAU, Lai-Kwan ,  TAY, Li-Lin ,  TANHA, Jamshid

Inventor： HUANG, Ping-Ji ,  CHAU, Lai-Kwan ,  TAY, Li-Lin ,  TANHA, Jamshid

IPC: C07K17/00 ,  C07K16/00 ,  C07K16/12 ,  C07K17/14 ,  G01N21/65 ,  G01N33/569

CPC classification number: C07K17/14 ,  C07K16/00 ,  C07K16/1271 ,  C07K2317/21 ,  C07K2317/569 ,  G01N21/658 ,  G01N33/54313 ,  G01N33/569 ,  G01N2333/205 ,  G01N2333/245 ,  G01N2333/25 ,  G01N2333/255 ,  G01N2333/31 ,  G01N2333/33 ,  Y10T428/2991

Abstract: A nanoaggregate embedded bead is formed from an inner core formed of comprising metallic nanoparticles and Raman active reporter molecules, an outer shell, and single-domain antibodies to target the bead to a specific target. The nanoaggregate embedded bead may be used in methods to detect analytes or pathogens in biological or environmental samples using Raman spectroscopy.

Abstract translation: 由包含金属纳米颗粒和拉曼活性报道分子，外壳和单结构域抗体形成的内核形成纳米聚集体包埋珠，以将珠靶向特定靶。 纳米聚集体嵌入珠可用于使用拉曼光谱法检测生物或环境样品中的分析物或病原体的方法。