公开(公告)号：WO2022157180A1

公开(公告)日：2022-07-28

申请号：PCT/EP2022/051100

申请日：2022-01-19

申请人： C-LECTA GMBH

发明人： DIRKMANN, Michael ,  VOGEL, Andreas ,  DUDEK, Hanna ,  BARTSCH, Sebastian ,  SCHILLING, Lena ,  CZAJA, Rico

IPC分类号： C12P5/00 ,  C12P7/02 ,  C12P7/26 ,  C12P7/40 ,  C12P9/00 ,  C12P17/04 ,  C12N9/02 ,  C12N9/10 ,  C12N9/12 ,  C12N9/90

摘要： The invention relates to process for isoprenoid production by an in-vitro synthetic platform involving at least the steps of phosphorylating a first hemiterpene alcohol with a first phosphate donor under catalysis of a first kinase thereby obtaining a first hemiterpene monophosphate and phosphorylating the latter with a second phosphate donor under catalysis of a second kinase thereby obtaining at least first hemiterpene diphosphate. The invention further relates to a composition comprising first hemiterpene diphosphate obtainable by the process according to the invention.

公开(公告)号：WO2016198665A1

公开(公告)日：2016-12-15

申请号：PCT/EP2016/063393

申请日：2016-06-10

申请人： C-LECTA GMBH

发明人： VOGEL, Andreas ,  SCHWARZE, Daniel ,  CZAJA, Rico ,  BAYER, Sally ,  BARTSCH, Sebastian

IPC分类号： C12N9/10

CPC分类号： C12N9/1096

摘要： The invention relates to transaminases and their use. The ATAs according to the invention are particularly useful for catalyzing the conversion of amines to ketones and/or vice versa. Preferably, the transaminase (ATA) according to the invention comprises an amino acid sequence with at least 80% homology to SEQ ID NO:1, wherein the amino acid sequence is engineered compared to SEQ ID NO:1 such that it comprises at least a substitution selected from the group consisting of F255L,F255A, F255C, F255D, F255E, F255G, F255H, F255K, F255M, F255N, F255P, F255Q, F255R, F255S, F255T, F255V, F255W, and F255Y.

摘要翻译： 本发明涉及转氨酶及其用途。 根据本发明的AT特别可用于催化胺转化为酮和/或反之亦然。 优选地，根据本发明的转氨酶（ATA）包含与SEQ ID NO：1具有至少80％同源性的氨基酸序列，其中与SEQ ID NO：1相比，氨基酸序列被工程化，使得其包含至少一个 选自F255L，F255A，F255C，F255D，F255E，F255G，F255H，F255K，F255M，F255N，F255P，F255Q，F255R，F255S，F255T，F255V，F255W和F255Y的取代基。

公开(公告)号：WO2018210794A1

公开(公告)日：2018-11-22

申请号：PCT/EP2018/062476

申请日：2018-05-15

申请人： C-LECTA GMBH

发明人： SCHÖNERT, Stefan ,  SALOMO, Mathias ,  SCHULTCHEN, Thomas ,  VOGEL, Andreas ,  KÖPKE, Sabrina ,  BARTSCH, Sebastian ,  BRUCHER, Birgit ,  FELLER, Claudia

IPC分类号： C12N9/00 ,  C07K1/14 ,  C12N1/08 ,  C12N9/10 ,  C12N9/22 ,  C07K1/34

摘要： The invention relates to a process for the manufacturing and purification of recombinant enzyme products, in particular of food enzyme products and the use thereof. The invention particularly relates to a process for the processing of enzyme products from a microbial fermentation broth by methods of separation, enzymatic treatment and filtration procedures.

公开(公告)号：WO2016198660A1

公开(公告)日：2016-12-15

申请号：PCT/EP2016/063387

申请日：2016-06-10

申请人： C-LECTA GMBH

发明人： VOGEL, Andreas ,  SCHWARZE, Daniel ,  CZAJA, Rico ,  BAYER, Sally ,  BARTSCH, Sebastian

IPC分类号： C12N9/10

CPC分类号： C12N9/1096

摘要： The invention relates to transaminases that are partucularly useful for catalyzing the conversion of amine substrates to ketone products and/or vice versa.

摘要翻译： 本发明涉及用于催化胺底物转化为酮产物和/或反之亦然的有用的转氨酶。

公开(公告)号：WO2019012095A1

公开(公告)日：2019-01-17

申请号：PCT/EP2018/069058

申请日：2018-07-13

申请人： C-LECTA GMBH

发明人： KÖPKE, Sabrina ,  BARTSCH, Sebastian ,  VOGEL, Andreas

IPC分类号： C12P33/00 ,  C12N9/04 ,  C12P7/38 ,  C12N9/00 ,  C12P1/00

摘要： The invention relates to ketoreductases and the use thereof. The ketoreductases of the invention are particularly useful for enzymatically catalyzing the reduction of ketones to chiral or non-chiral secondary alcohols and oxidation of chiral or non-chiral secondary alcohols to ketones.