摘要:
The invention relates to process for isoprenoid production by an in-vitro synthetic platform involving at least the steps of phosphorylating a first hemiterpene alcohol with a first phosphate donor under catalysis of a first kinase thereby obtaining a first hemiterpene monophosphate and phosphorylating the latter with a second phosphate donor under catalysis of a second kinase thereby obtaining at least first hemiterpene diphosphate. The invention further relates to a composition comprising first hemiterpene diphosphate obtainable by the process according to the invention.
摘要:
Die vorliegende Erfindung befindet sich auf dem Gebiet der Herstellung von Lebens- und Genussmitteln, insbesondere von Kaffee- und Kaffeeersatzprodukten mit verringertem Acrylamidgehalt. Es wird ein Verfahren zur Entfernung von Acrylamid aus verschiedenen Lebensmittelmatrices, insbesondere aus einer Kaffee- oder Kaffeeersatzproduktmatrix bereitgestellt, welches die Verwendung eines Biokatalysators zum Abbau von Acrylamid beinhaltet.
摘要:
The invention relates to the enzymatic treatment of a virgin liquid nutrient naturally containing carbohydrates for the in-situ production of functional carbohydrates, thereby obtaining a fortified processed liquid nutrient, being rich (or enriched) in such functional carbohydrates and offering a beneficial nutritional value. The invention relates to the in-situ use of enzymes during food processing of a virgin liquid nutrient for the preparation of fortified food containing supplementary functional carbohydrates of specified composition.
摘要:
The present invention relates to a sucrose phosphorylase variant comprising or consisting of an amino acid sequence with at least 75% homologous and/or identical to an amino acid sequence of wild type sucrose phosphorylase, preferably wherein the sucrose phosphorylase is characterized by different structural and functional features. The invention also relates to method for preparing aG1 P and a coproduct, wherein the method comprises reacting a sucrose phosphorylase with a glucoside substrate, wherein the sucrose phosphorylase is a sucrose phosphorylase as defined in the context of the present invention.
摘要:
The invention relates to ketoreductases and the use thereof. The ketoreductases of the invention are particularly useful for enzymatically catalyzing the reduction of ketones to chiral or non-chiral secondary alcohols and oxidation of chiral or non-chiral secondary alcohols to ketones.
摘要:
The present invention relates to a method for producing trehalose, comprising the steps of mixing and reacting, in any order, (i) at least one alpha-phosphorylase capable of catalyzing the production of alpha-D-glucose 1-phosphate intermediate from a saccharide raw material, and from at least one phosphorus source;(ii) at least one trehalose phosphorylase capable of catalyzing the production of trehalose from an alpha-D-glucose 1-phosphate intermediate and a glucose substrate, wherein the trehalose phosphorylase is a trehalose phosphorylase variant with an amino acid sequence which differs from the amino acid sequence of a wild type trehalose phosphorylase in at least one amino acid position, (iii) at least one saccharide raw material which produces an alpha-D-glucose 1-phosphate intermediate and a co-product by catalytic action of the alpha-phosphorylase; and (iv) at least one phosphorus source selected from the group consisting of a phosphoric acids and an inorganic salt thereof.
摘要:
The present invention is related to a trehalose phosphorylase comprising an amino acid sequence, wherein the amino acid sequence of the trehalose phosphorylase is at least 80% identical to and/or at least 80% homologous to an amino acid sequence of SEQ ID NO:1, wherein the amino acid sequence of the trehalose phosphorylase comprises an amino acid substitution at one or more amino acid positions, wherein the one or more amino acid positions is/are selected from the group consisting of amino acid positions of SEQ ID NO: 1 712, 383, 10, 114, 118, 192, 197, 220, 225, 304, 306, 318, 323, 339, 349, 357, 459, 476, 481, 484, 487, 488, 506, 511, 526, 530, 532, 533, 537, 550, 556, 564, 590, 649, 667, 703 and 705.
摘要:
The use of insoluble enzyme preparations comprising an enzyme which is immobilized on a solid carrier having an average particle size of from about 0.1 to 500 µm as additives in consumer near applications is disclosed. These insoluble enzyme preparations provide compositions with reduced safety risks regarding allergenicity compared to the free enzyme. Therefore, the insoluble enzyme preparations are beneficial when used in certain applications that involve direct contact of enzymes with humans either by handling, utilizing, or uptake of enzymes or enzyme containing compositions. Furthermore, the insoluble enzyme preparations have low dusting, high blendability and good dispersion characteristics which are particularly beneficial to numerous industries (including, but not limited to, personal care, oral care, home care, food and/or feed industries). The invention further relates to the use of insoluble enzyme preparations dispersed in single-use compositions which may be e.g. chewing gum, toothpaste, ointment, shampoo, deodorant, a silicone elastomer, dry provender for animal nutrition, a bakery composition like dough, paint or a home care composition like hard surface cleaner.
摘要:
Die Erfindung betrifft eine Nitrilase mit verbesserter Aktivität bei der Umsetzung eines Nitrils zur entsprechenden Carbonsäure, insbesondere im Hinblick auf die Umsetzung von 2- Methylglutaronitril, 1-(Cyanomethyl)cyclohexan-1-carbonitril und Benzonitril. Die erfindungsgemäße Nitrilase ist der Nitrilase aus Acidovorax facilis verwandt.