公开(公告)号：WO1987004181A1

公开(公告)日：1987-07-16

申请号：PCT/US1987000044

申请日：1987-01-05

Applicant: CALGENE, INC. ,  STALKER, David, M.

Inventor： CALGENE, INC.

IPC: C12N01/00

CPC classification number: C12N15/63 ,  C12N9/78 ,  C12N15/64 ,  C12N15/70 ,  C12N15/8274

Abstract: Nitrilase enzymes specific for the hydrolysis of the nitrile group of bromoxynil, nucleotide sequences encoding for such enzymes, and transformed cells in which the nitrilase expression is foreign. The transformed cells are capable of expressing the nitrilase enzyme to provide detoxification of an environment and protect bromoxynil-sensitive cells from its cytotoxic effect. Particularly, plants are developed which are resistant to bromoxynil.

Abstract translation: 对溴苯腈的腈基进行水解的腈水解酶，编码这些酶的核苷酸序列以及腈水解酶表达是外来的转化细胞。 转化细胞能够表达腈水解酶以提供环境的解毒，并保护溴苯腈敏感细胞免受其细胞毒性作用。 特别地，开发出抗溴苯腈耐受性的植物。

公开(公告)号：WO1998018949A2

公开(公告)日：1998-05-07

申请号：PCT/US1997019529

申请日：1997-10-29

Applicant: CALGENE, INC. ,  STALKER, David, M. ,  PEAR, Julie, R. ,  DELMER, Deborah, P.

Inventor： CALGENE, INC.

IPC: C12N15/82

CPC classification number: C12N9/1059 ,  C12N15/8222 ,  C12N15/8233 ,  C12N15/8242 ,  C12N15/8246 ,  C12N15/8261 ,  Y02A40/146

Abstract: Provided are two plant cDNA clones that are homologs of the bacterial CelA genes that encode the catalytic subunit of cellulose synthase, derived from cotton (Gossypium hirsutum). Also provided are genomic promoter regions to these encoding regions to cellulose synthase. Methods for using cellulose synthase in cotton fiber and wood quality modification are also provided.

Abstract translation: 提供两个植物cDNA克隆，其是编码来自棉花（Gossypium hirsutum）的纤维素合酶的催化亚单位的细菌CelA基因的同系物。 还提供了这些编码区到纤维素合成酶的基因启动子区。 还提供了在棉纤维中使用纤维素合成酶和木质素改性的方法。

公开(公告)号：WO1996040924A2

公开(公告)日：1996-12-19

申请号：PCT/US1996009897

申请日：1996-06-07

Applicant: CALGENE, INC. ,  McBRIDE, Kevin ,  STALKER, David, M. ,  PEAR, Julie, R. ,  PEREZ-GRAU, Luis

Inventor： CALGENE, INC.

IPC: C12N15/29

CPC classification number: C12N9/0071 ,  C07K14/36 ,  C07K14/415 ,  C12N9/0069 ,  C12N9/88 ,  C12N15/8233 ,  C12N15/825 ,  C12N15/8261 ,  Y02A40/146

Abstract: Novel DNA constructs are provided which may be used as molecular probes or inserted into a plant host to provide for modification of transcription of a DNA sequence of interest during various stages of cotton fiber development. The DNA constructs comprise a cotton fiber transcriptional initiation regulatory region associated with a gene which is expressed in cotton fiber. Also provided is novel cotton having a cotton fiber which has a natural color introduced by the expression in the cotton fiber cell, using such a construct, of pigment synthesis genes. Cotton fiber cells having color produced by genetic engineering and cotton cells comprising melanin and indigo pigments are included.

Abstract translation: 提供新的DNA构建体，其可以用作分子探针或插入到植物宿主中，以提供在棉花纤维发育的各个阶段期间感兴趣的DNA序列的转录的修饰。 DNA构建体包含与在棉纤维中表达的基因相关的棉花纤维转录起始调节区。 还提供了具有棉纤维的新型棉，其具有通过色素合成基因使用这种构建体在棉纤维细胞中表达引入的天然色。 包括由基因工程产生的颜色的棉纤维细胞和包含黑色素和靛蓝色素的棉花细胞。

公开(公告)号：WO1991019808A1

公开(公告)日：1991-12-26

申请号：PCT/US1991004116

申请日：1991-06-11

Applicant: CALGENE, INC. ,  STALKER, David, M. ,  SHEWMAKER, Christine, K. ,  OAKES, Janette, V.

Inventor： CALGENE, INC.

IPC: C12P19/18

CPC classification number: C12N9/1074 ,  C12N15/8245 ,  C12P19/18

Abstract: The present invention is directed to the production of cyclodextrins in plants. Specifically, it has been found that host plants can be successfully transformed with a DNA sequence capable of expressing cyclodextrin glycosyltransferase which will convert the plants' endogenous starch reserves to cyclodextrins.

Abstract translation: 本发明涉及植物中环糊精的生产。 具体地说，已经发现宿主植物可以用能够表达环糊精糖基转移酶的DNA序列成功转化，这将使植物的内源性淀粉储备转化为环糊精。

公开(公告)号：WO1996036719A1

公开(公告)日：1996-11-21

申请号：PCT/US1996007064

申请日：1996-05-15

Applicant: CALGENE, INC.

Inventor： CALGENE, INC. ,  YUAN, Ling

IPC: C12N15/55

CPC classification number: C12N15/8247 ,  C12N9/16

Abstract: Methods of altering substrate specificity of plant acyl-ACP thioesterases, and engineered plant acyl-ACP thioesterases so produced are provided. The C-terminal two-thirds portion of plant thioesterases is identified as desirable for such modifications. DNA sequences and constructs for expression of engineered thioesterases, as well as the novel thioesterases produced therefrom are also provided. Such DNA sequences may be used for expression of the engineered thioesterases in host cells, particularly seed cells of oilseed crop plants, for the modification of fatty acid composition. A C12 preferring plant acyl-ACP thioesterase described herein may be altered to obtain a plant thioesterase having approximately equal activity on C14 and C12 substrates. Further modification of the C12 enzyme yields a thioesterase having greater activity on C14 as compared to C12 substrates.

Abstract translation: 提供了改变植物酰基-ACP硫酯酶底物特异性的方法，以及如此制备的工程化植物酰基-ACP硫酯酶。 植物硫酯酶的C末端三分之二部分被鉴定为对于这种修饰是理想的。 还提供了用于表达工程化硫酯酶的DNA序列和构建体，以及由其产生的新的硫酯酶。 这样的DNA序列可用于在宿主细胞，特别是油菜作物植物的种子细胞中表达工程改造的硫酯酶，用于改变脂肪酸组成。 可以改变本文所述的C12优选植物酰基-ACP硫酯酶以获得对C14和C12底物具有大致相同活性的植物硫酯酶。 与C12底物相比，C12酶的进一步修饰产生对C14具有更大活性的硫酯酶。

公开(公告)号：WO1996021738A1

公开(公告)日：1996-07-18

申请号：PCT/US1996000562

申请日：1996-01-16

Applicant: CALGENE, INC.

Inventor： CALGENE, INC. ,  SHEWMAKER, Christine, K.

IPC: C12N15/82

CPC classification number: C12N9/1029 ,  C12N15/8245

Abstract: This invention relates to methods for the utilization of sucrose phosphate synthase encoding sequences to modify the soluble solids in plant sink tissue. The method permits an increase in the sweetness of tomato fruit.

Abstract translation: 本发明涉及利用蔗糖磷酸合成酶编码序列来修饰植物汇组织中的可溶性固体的方法。 该方法可以增加番茄果实的甜度。

公开(公告)号：WO1993010241A1

公开(公告)日：1993-05-27

申请号：PCT/US1992009863

申请日：1992-11-13

Applicant: CALGENE, INC. ,  METZ, James, George ,  LARDIZABAL, Kathryn, Dennis ,  LASSNER, Michael, W.

Inventor： CALGENE, INC.

IPC: C12N15/54

CPC classification number: C12N9/0008 ,  C12N9/1029 ,  C12N15/8247 ,  C12P7/6463 ,  C12Y102/01042

Abstract: By this invention, a partially purified fatty acyl-CoA: fatty alcohol acyltransferase (wax synthase) is provided, wherein said protein is active in the formation of a wax ester from fatty alcohol and fatty acyl substrates. Of special interest is a jojoba embryo wax synthase having an apparent molecular mass of approximately 57kD. Also considered are amino acid and nucleic acid sequences obtainable from wax synthase proteins and the use of such sequences to provide transgenic host cells capable of producing wax esters.

Abstract translation: 通过本发明，提供部分纯化的脂肪酰基-CoA：脂肪醇酰基转移酶（蜡合酶），其中所述蛋白质在脂肪醇和脂肪酰基底物形成蜡酯中是有活性的。 特别感兴趣的是具有约57kD的表观分子量的霍霍巴胚蜡合成酶。 还考虑了可从蜡合酶蛋白获得的氨基酸和核酸序列，以及使用这些序列提供能够生产蜡酯的转基因宿主细胞。

公开(公告)号：WO1992003564A1

公开(公告)日：1992-03-05

申请号：PCT/US1991005801

申请日：1991-08-15

Applicant: CALGENE, INC. ,  KNAUF, Vic, C. ,  THOMPSON, Gregory, A.

Inventor： CALGENE, INC.

IPC: C12P07/64

CPC classification number: C12N9/0083 ,  C12N9/1029 ,  C12N15/52 ,  C12N15/8247 ,  C12P7/6463 ,  C12Y114/19002

Abstract: By this invention, compositions and methods of use related to beta -ketoacyl-ACP synthase, hereinafter also referred to as ''synthase'', are provided. Also of interest are methods and compositions of amino acid and nucleic acid sequences related to biologically active plant synthase(s). In particular, synthase protein preparations which have relatively high turnover (specific activity) are of interest for use in a variety of applications, in vitro and in vivo. Especially, protein preparations having synthase I and/or synthase II activities are contemplated hereunder. Synthase activities are distinguished by the preferential activity towards longer and shorter acyl-ACPs. Protein preparations having preferential activity towards shorter chain length acyl-ACPs are synthase I-type. Synthases having preferential activity towards longer chain length acyl-ACPs are synthase II-type. Of special interest are synthases obtainable from Ricinus communis.

Abstract translation: 通过本发明，提供了与β-酮酰基-ACP合酶相关的组合物和使用方法，以下也称为“合成酶”。 还感兴趣的是与生物活性植物合酶相关的氨基酸和核酸序列的方法和组合物。 特别地，具有相对高的周转率（比活性）的合成酶蛋白制剂在体外和体内用于各种应用中是有意义的。 特别地，考虑到具有合酶I和/或合酶II活性的蛋白质制剂。 合酶活性的区别在于对较长和较短酰基-ACP的优先活性。 对较短链长酰基-ACP具有优先活性的蛋白质制剂是合酶I型。 对较长链长酰基-ACP具有优先活性的合成酶是合酶II型。 特别感兴趣的是从蓖麻（Ricinus communis）获得的合成酶。

公开(公告)号：WO1991016421A1

公开(公告)日：1991-10-31

申请号：PCT/US1991002960

申请日：1991-04-25

Applicant: CALGENE, INC. ,  DAVIES, Huw, Maelor ,  POLLARD, Michael, Roman ,  VOELKER, Toni, Alois ,  THOMPSON, Gregory, A.

Inventor： CALGENE, INC.

IPC: C12N09/14

CPC classification number: C12P7/6418 ,  C07K14/415 ,  C12N9/16 ,  C12N15/8247 ,  C12P7/6463

Abstract: This invention relates to plant thioesterases, means to identify such proteins, amino acid and nucleic acid sequences associated with such protein, methods to obtain, make and/or use such plant thioesterases. Also, by this invention, the existence of a heretofore unproven factor critical to the biosynthesis of medium-chain fatty acids in plants is demonstrated.

Abstract translation: 本发明涉及植物硫酯酶，鉴定此类蛋白质的方法，与这种蛋白质相关的氨基酸和核酸序列，获得，制备和/或使用这种植物硫酯酶的方法。 此外，通过本发明，证明了对植物中的中链脂肪酸的生物合成关键的迄今未证明的因素的存在。

公开(公告)号：WO1991013980A1

公开(公告)日：1991-09-19

申请号：PCT/US1991001750

申请日：1991-03-14

Applicant: CALGENE, INC.

Inventor： CALGENE, INC. ,  KNAUF, Vic, C. ,  KRIDL, Jean, C. ,  SCHERER, Donna, E.

IPC: C12N15/11

CPC classification number: C07K14/415 ,  C12N15/8222 ,  C12N15/8234

Abstract: Novel DNA constructs which may be used as molecular probes or inserted into a plant host are provided. These constructs comprise a sequence obtainable from the Bce4 gene that is capable of directing transcription in seed tissue at least as early as 11 days after anthesis until approximately 30-35 days after anthesis, joined to a nucleic acid sequence of interest, and a transcription termination region. Thus, transcription of a message encoded by a nucleic acid sequence under the control of the Bce4 regulatory region will occur at a specific time of seed development. In this manner, production of exogenous products, as well as modulation of endogenous products, may be achieved.

Abstract translation: 提供了可用作分子探针或插入植物宿主的新型DNA构建体。 这些构建体包含可从Bce4基因获得的序列，其能够至少早于开花后11天直到种子组织中的转录，直到开花后约30-35天，连接到感兴趣的核酸序列和转录终止 地区。 因此，由Bce4调控区域控制下的核酸序列编码的信息的转录将在种子发育的特定时间发生。 以这种方式，可以实现外源产物的生产以及内源产物的调节。