公开(公告)号：WO1998016624A1

公开(公告)日：1998-04-23

申请号：PCT/DE1997002277

申请日：1997-10-04

Applicant: BUNA SOW LEUNA OLEFINVERBUND GMBH ,  LEHMANN, Olaf ,  BERNDT, Christiane ,  MENZEL, Klaus-Dieter ,  DRIESCH, Dominik ,  WULFES, Christian ,  GLIEM, Toralf ,  CHRISTNER, Arnulf ,  HILLIGER, Matthias ,  MIERSCH, Karl

Inventor： BUNA SOW LEUNA OLEFINVERBUND GMBH

IPC: C12N01/00

CPC classification number: C12N1/32

Abstract: Methylotrophic micro-organisms are cultivated in known manner on the carbon substrate, methanol, using continuous measuring and secondary feeding processes in order to restrict the methanol concentration to very low values. According to the invention, a cultivation method comprising a plurality of cultivation stages is carried out in agitated and aerated bioreactors, the average methanol concentration decreasing from one stage to the next owing to suitable feeding methods. To that end, the methanol fed in during the cultivation stage (k-2) is proportional to the alkaline solution added in metered amounts during the regulation of the pH, in the cultivation stage (k-1), as the specific dosage, and in the final cultivation stage (k) first as the specific and then as the pO2-regulated dosage. This method prevents an accumulation of toxic intermediates and avoids the need to measure and regulate the methanol concentration during the entire cultivation period, such that costs are considerably reduced, in particular when the method is applied on an industrial scale.

Abstract translation: 在使用连续测量和Nachfütterungsverfahren以已知的方式在碳基材甲醇的甲基的微生物的培养来限制到非常低的值的甲醇浓度。 根据本发明，在描述和搅拌的生物反应器充气其中由合适Zufütterungsmethoden甲醇浓度从阶段平均降低至阶段的k阶段培养过程。 该Methanolzufütterung在培育阶段（K-2）成比例，在培育阶段中pH控制液中的剂量（K-1）作为施加的剂量和在最终的培养步骤（k）作为第一压花和进一步氧分压 控制用量。 该方法防止了有毒的中间体的累积。 整个培养过程中不需要的测量和控制甲醇的浓度。 这使得该方法在一个显著成本降低有助于特别是在工业规模上。

公开(公告)号：WO1996040861A1

公开(公告)日：1996-12-19

申请号：PCT/US1996007904

申请日：1996-05-29

Applicant: BIOLOG, INC.

Inventor： BIOLOG, INC. ,  BOCHNER, Barry

IPC: C12N01/00

CPC classification number: C12Q1/04 ,  C12N1/20 ,  C12N1/38 ,  C12Q1/10 ,  C12Q2334/50 ,  C12Q2334/52

Abstract: The present invention is directed to methods and media for the growth, enrichment, isolation, and presumptive identification of enteric pathogens such as E. coli 0157:H7 and Salmonella. In particular, the organisms commonly associated with gastrointestinal infections of humans and other animals are distinguished based on their growth, colonial morphology and color. The present invention is also directed to methods and media for the growth, enrichment, isolation and presumptive identification of enteric pathogens such as E. coli 0157:H7 and Salmonella isolated from food, water, dairy, and environmental samples.

Abstract translation: 本发明涉及肠道病原体如大肠杆菌0157：H7和沙门氏菌的生长，富集，分离和推测鉴定的方法和培养基。 特别地，通常与人类和其他动物的胃肠道感染相关的生物基于它们的生长，殖民地形态和颜色来区分。 本发明还涉及从食品，水，乳制品和环境样品分离的大肠杆菌0157：H7和沙门氏菌的肠道病原体的生长，富集，分离和推定鉴定的方法和培养基。

公开(公告)号：WO1996038534A1

公开(公告)日：1996-12-05

申请号：PCT/US1996007538

申请日：1996-05-28

Applicant: HENKEL CORPORATION

Inventor： HENKEL CORPORATION ,  ANDERSON, Kevin, W. ,  WENZEL, J., Douglas

IPC: C12N01/00

CPC classification number: C12P7/6418

Abstract: Carboxylic acids and glycerine are made by a continuous splitting process which involves the formation of a presplitting mixture by separately adding the glyceride, and effective lipase in an amount sufficient to produce partial splitting of the glyceride, and water. The water used in the formation of the presplitting mixture is water that has been separated from the glycerine-water effluent stream from the pressure splitter and recycled. The next step involves the pressure splitting which entails mixing the partially split glyceride from the presplitter with water and heating under conditions of temperature and pressure effective to substantially complete the splitting of the glyceride into component fatty acids and a glycerine-water stream. The water is then separated from the glycerine-water stream and the water is recycled to the presplitter.

Abstract translation: 羧酸和甘油通过连续裂解方法制备，该方法涉及通过分开加入甘油酯形成预分解混合物，以及足以产生甘油酯和水的部分分裂的有效脂肪酶。 用于形成预分裂混合物的水是已经从压力分配器从甘油 - 水流出物流中分离出来并再循环的水。 下一步涉及压力分解，其需要将来自分解器的部分分离的甘油酯与水混合并在温度和压力的条件下加热，以有效地基本上完成甘油酯分解成组分脂肪酸和甘油 - 水流。 然后将水从甘油 - 水流中分离出来并将水再循环到分解器中。

公开(公告)号：WO1996011258A1

公开(公告)日：1996-04-18

申请号：PCT/US1995013196

申请日：1995-10-04

Applicant: MICROCARB, INC.

Inventor： MICROCARB, INC. ,  PACE, John, L. ,  WALKER, Richard, I. ,  FREY, Steven, M.

IPC: C12N01/00

CPC classification number: A61K39/105 ,  A61K39/025 ,  A61K39/09 ,  A61K39/107 ,  A61K2039/55555 ,  C12N1/20 ,  C12Q1/045 ,  C12Q1/18 ,  Y02A50/47 ,  Y02A50/472

Abstract: Methods using in vitro processes are disclosed for inducing or enhancing expression of enteric bacterial antigens or virulence factors. The methods, therefore, produce antigenically enhanced enteric bacteria. Also methods for using the antigenically enhanced bacteria are also disclosed, as well as vaccines containing the enteric bacteria. Specifically, a whole enteric bacteria or components thereof are provided by Helicobacter species. Also there are other enteric bacteria which are useful for the disclosed invention. One type, Campylobacter jejuni is graphically depicted wherein the results of high-performance liquid chromatography of monosaccharides from surface extract hydrolysates of C. jejuni are shown.

Abstract translation: 公开了使用体外方法诱导或增强肠细菌抗原或毒力因子表达的方法。 因此，该方法产生抗原性增强的肠细菌。 还公开了使用抗原性增强的细菌的方法，以及含有肠细菌的疫苗。 具体地，整个肠道细菌或其组分由幽门螺杆菌属物种提供。 还有其它肠道细菌可用于所公开的发明。 一种类型的空肠弯曲杆菌被图形化，其中显示了来自空肠弯曲杆菌的表面提取物水解产物的单糖的高效液相色谱的结果。

公开(公告)号：WO1995004814A1

公开(公告)日：1995-02-16

申请号：PCT/US1994008846

申请日：1994-08-05

Applicant: INTERNATIONAL TLB RESEARCH INSTITUTE, INC.

Inventor： INTERNATIONAL TLB RESEARCH INSTITUTE, INC. ,  ZHANG, Ling, Yu

IPC: C12N01/00

CPC classification number: C05F11/08 ,  C07K14/225 ,  C07K14/32 ,  C07K14/395 ,  C12N15/76

Abstract: A microbial fertilizer that constitutes a symbiotic association of several recombinant microbial species is described. The fertilizer contains four streptomyces strains (5 and 8) and two yeast strains (H4 (1) and H7 (3)). The streptomyces strains include a nitrogen fixing strain, a phosphorus decomposer, a potassium decomposer and a coal waste decomposer. The yeast strains produce growth factors and energy required by the streptomyces.

Abstract translation: 描述了构成几种重组微生物物种的共生关联的微生物肥料。 肥料含有四种链霉菌菌株（5和8）和两种酵母菌株（H4（1）和H7（3））。 链霉菌菌株包括固氮菌株，磷分解器，钾分解器和煤废物分解器。 酵母菌株产生链霉菌所需的生长因子和能量。

公开(公告)号：WO1995004131A1

公开(公告)日：1995-02-09

申请号：PCT/JP1994001245

申请日：1994-07-28

Applicant: NIPPON SEISHI KABUSHIKI KAISHA ,  IIMORI, Takeshi ,  KANEKO, Reiji ,  YOSHIKAWA, Hiroshi ,  MACHIDA, Makoto

Inventor： NIPPON SEISHI KABUSHIKI KAISHA

IPC: C12N01/00

CPC classification number: C12R1/645 ,  D21C5/005

Abstract: A method of bleaching pulp using a microbe SKB-1152 strain, which is a lignin-decomposing bacterium having a high lignin-decomposing activity and a high activity of high-temperature pulp bleaching, or a product of culturing the microbe. The above method can be performed by the conventional biobleaching process without the necessity for employing special culture conditions. This strain can be obtained from a declorated zone formed in the medium prepared by inoculating a sample strain in a medium containing lignin and/or pulp wherein lignin remains.

Abstract translation: 使用具有高木质素分解活性和高温纸浆漂白活性的木质素分解细菌的微生物SKB-1152菌株或培养微生物的产物漂白纸浆的方法。 上述方法可以通过常规生物漂白方法进行，而不需要使用特殊培养条件。 该菌株可以从通过将样品菌株接种在含木质素和/或木质的培养基中制备的培养基中形成的分解区获得，其中木质素保留。

公开(公告)号：WO1994013782A1

公开(公告)日：1994-06-23

申请号：PCT/GB1993002568

申请日：1993-12-16

Applicant: BIOTECHNA LIMITED ,  HUGHES, Martin, Neville ,  POOLE, Robert, Keith ,  BENNETT, Sarah, Melanie

Inventor： BIOTECHNA LIMITED

IPC: C12N01/00

CPC classification number: C12N1/005 ,  A23K10/16 ,  A23L29/065 ,  A23L33/16 ,  B01J20/24 ,  C02F1/286 ,  C02F3/327 ,  C02F3/34 ,  Y02W10/18

Abstract: Microbial biomass cells wherein at least one metal cationic species is attached to the surface of said cells in an amount sufficient to render the said cells effective to bind anions thereto, and wherein at least one anionic species is also bound to the surface of said cells. The invention also provides a method of making microbial biomass cells as claimed in any preceding claims which comprises: providing biomass cells within a culture medium, removing said cells from said medium and washing them, suspending the cells in a buffer medium, adding a quantity of the required cationic species in aqueous solution optionally to saturation of the binding sites therefor on the cell wall, adding a quantity of the required anionic species in aqueous solution optionally to saturation of the binding sites therefor on the cell wall, removing the treated biomass and washing, and drying the cells to form a solid product. A digestible, mineral-containing composition is also provided which contains biomass cells. For environmental clean-up applications there is also provided a method of reducing the concentration of at least one anionic species in an aqueous composition containing said species, which comprises bringing said aqueous composition into intimate contact with microbial biomass cells, to which at least one metal cationic species has been bound in a quantity sufficient to render said biomass cells receptive to bind said at least one anionic species, and optionally causing the said biomass cells to become regenerated with the same or different metal cationic species after anionic species have become bound to said biomass.

Abstract translation: 微生物生物质细胞，其中至少一种金属阳离子物质以足以使所述细胞有效结合阴离子的量连接到所述细胞的表面，并且其中至少一种阴离子物质也结合到所述细胞的表面。 本发明还提供了一种制备如前述权利要求中任一项所述的微生物生物细胞的方法，其包括：在培养基内提供生物质细胞，从所述培养基中除去所述细胞并洗涤，将细胞悬浮在缓冲介质中，加入一定量的 水溶液中所需的阳离子物质任选地在细胞壁上饱和其结合位点，在水溶液中加入一定量的所需阴离子物质，任选地饱和其在细胞壁上的结合位点，除去处理的生物质和洗涤 ，并干燥细胞以形成固体产物。 还提供含有生物质细胞的可消化的含矿物质组合物。 对于环境清理应用，还提供了降低含有所述物质的水性组合物中至少一种阴离子物质的浓度的方法，其包括使所述水性组合物与微生物生物质细胞紧密接触，至少一种金属 阳离子物质已经被结合到足以使所述生物质细胞接受结合所述至少一种阴离子物质的量，并且任选地使所述生物质细胞在阴离子物质已经结合到所述生物质细胞之后用相同或不同的金属阳离子物质再生 生物质。

公开(公告)号：WO1992019717A1

公开(公告)日：1992-11-12

申请号：PCT/US1992002798

申请日：1992-04-01

Applicant: BAYLOR COLLEGE OF MEDICINE ,  GES PHARMACEUTICALS, INC.

Inventor： BAYLOR COLLEGE OF MEDICINE ,  GES PHARMACEUTICALS, INC. ,  CHANG, Albert, S. ,  HIBI, Nozomu ,  KAWAMATA, Osamu

IPC: C12N01/00

CPC classification number: G01N33/5008

Abstract: Human neurotumor cells capable of being grown in culture and having a specific high-affinity glutamate/aspartate transport system are provided. Further, is a clonal derivation cell line SH-EP having a flat epithelial morphology and has a specific high-affinity Glutamate/Aspartate transport system. Methods are provided for deriving the SH-EP cells and for using SH-EP cells to detect agonists and antagonists for a glutamate/aspartate transport system. The transport and pharmacological characteristics of this cell line are described.

Abstract translation: 提供能够在培养物中生长并具有特异性高亲和力谷氨酸/天冬氨酸转运系统的人类神经肿瘤细胞。 此外，是具有平坦上皮形态并具有特异性高亲和力谷氨酸/天门冬氨酸转运系统的克隆衍生细胞系SH-EP。 提供了用于获得SH-EP细胞和使用SH-EP细胞检测谷氨酸/天冬氨酸转运系统的激动剂和拮抗剂的方法。 描述了该细胞系的运输和药理学特征。

公开(公告)号：WO1992013941A1

公开(公告)日：1992-08-20

申请号：PCT/US1992000901

申请日：1992-02-05

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  WILCOX, David, R. ,  SMITH, Robert, A. ,  BENSON, Terry, A.

IPC: C12N01/00

CPC classification number: C12R1/075 ,  A01N63/00 ,  C07K14/325

Abstract: Novel bacterial isolates of B. thuringiensis are having enhanced toxicity with respect to previously resistant or insufficiently susceptible insect species, including, but not limited to, Plutella xylostella, Spodoptera frugiperda and Spodoptera exigua, as well as certain secondary pests such as Trichoplusia ni. Such isolates may be characterized by their possession of a particular subset of the genes coding for the various B. thuringiensis delta -endotoxin proteins and by a characteristic plasmid profile, or array, known to be associated therewith. Also disclosed are a method for the efficient identification of such bacterial isolates utilizing generalized or, alternatively, gene-specific DNA probes; cloned or synthesized nucleotide sequences which are useful in the preparation of those probes; compositions containing an insecticidally effective amount of a bacterial isolate of the invention in combination with an acceptable carrier; and a method for the use thereof in the control or eradication of insect pests.

Abstract translation: 苏云金芽孢杆菌的新型细菌分离物对先前抗性或不充分易感的昆虫物种具有增强的毒性，包括但不限于小菜蛾，草地贪夜蛾和草地夜蛾，以及某些次生害虫如毛滴虫（Trichoplusia ni）。 这些分离物的特征在于它们具有编码各种苏云金芽孢杆菌δ-内毒素蛋白质的基因的特定子集，以及已知与之相关的特征性质粒谱或阵列。 还公开了利用广泛的或者基因特异性DNA探针有效鉴定这种细菌分离物的方法; 克隆或合成的可用于制备这些探针的核苷酸序列; 含有杀虫有效量的本发明细菌分离物与可接受载体组合的组合物; 以及用于控制或根除害虫的方法。

公开(公告)号：WO1986006742A1

公开(公告)日：1986-11-20

申请号：PCT/US1986001002

申请日：1986-05-07

Applicant: CALIFORNIA BIOTECHNOLOGY INC.

Inventor： CALIFORNIA BIOTECHNOLOGY INC. ,  SCHENK, Dale, B. ,  SPRATT, Sharon, Kaye

IPC: C12N01/00

CPC classification number: C07K14/005 ,  C07K14/58 ,  C07K14/785 ,  C07K2319/00 ,  C07K2319/40 ,  C07K2319/61 ,  C12N9/2468 ,  C12N2760/16022 ,  G01N33/535

Abstract: A fused protein for use in an enzyme immunoassay system. The protein comprises an enzymatically active beta -galactosidase fused, at its C terminus, to an immunologically active peptide. The protein is produced using a plasmid containing a complete beta -galactosidase gene fused, at its 3' end, with an oligonucleotide coding for the peptide. The fused protein is designed for use in a solid-phase enzyme immunoassay system, based on immunospecific binding of the fused protein to a solid support, or in a homogeneous enzyme immunoassay system, based on enzyme inhibition resulting from immunospecific binding of an antibody to the protein.

Abstract translation: 用于酶免疫测定系统的融合蛋白。 蛋白质包含在其C末端融合到免疫活性肽的酶活性β-半乳糖苷酶。 使用含有完整的β-半乳糖苷酶基因的质粒在其3'末端与编码该肽的寡核苷酸融合来产生该蛋白质。 融合蛋白被设计用于固相酶免疫测定系统，基于融合蛋白与固体支持物的免疫特异性结合，或在均相酶免疫测定系统中，基于由抗体与抗体的免疫特异性结合产生的酶抑制 蛋白。