公开(公告)号：WO2020117713A1

公开(公告)日：2020-06-11

申请号：PCT/US2019/064071

申请日：2019-12-02

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： ASKARY, Amjad ,  ELOWITZ, Michael, B. ,  BUDDE, Mark, W. ,  LOIS, Carlos ,  GUARDADO, Luis, Sanchez ,  CAI, Long ,  LINTON, James

IPC: C12Q1/6813

Abstract: Disclosed herein include systems, methods, compositions, and kits for in situ readout of barcodes, such as DNA barcodes. Barcode constructs containing a promoter (e.g., a phage promoter) that is inactive in live cells can be integrated in the genomes of cells. Cells can be fixed, and phage RNA polymerase can be used for transcription of the barcode to RNA transcripts. The RNA transcripts can be detected using, for example, fluorescent imaging and used to determine barcode sequences.