公开(公告)号：WO2021140129A1

公开(公告)日：2021-07-15

申请号：PCT/EP2021/050143

申请日：2021-01-06

Applicant: CARROUCELL

Inventor： FATHALLAH, Tarek

IPC: C12M1/12 ,  C12N5/00 ,  C12M25/16 ,  C12N5/0075

Abstract: Procédé de formation de microporteurs (1), comportant: a) formation de gouttes liquides (12) à partir d'une solution sol-gel (2); b) dépôts des gouttes, sous forme de liquide, sur un premier support (10), de préférence hydrophobe; c) déformation des gouttes déposées sur le premier support (10); d) solidification des gouttes par gélification et séchage, de façon à former des microporteurs solides; e) extraction des microporteurs solidifiés du premier support (10).

公开(公告)号：WO2018050660A1

公开(公告)日：2018-03-22

申请号：PCT/EP2017/072940

申请日：2017-09-12

Applicant: CARROUCELL

Inventor： FATHALLAH, Tarek ,  FERGUSON, Michael

IPC: C12M1/12 ,  B01J20/10 ,  C03C1/00 ,  C12N5/00 ,  C12M3/00

Abstract: The invention relates to a process for producing a glass macroporous matrix, comprising the steps of : 1 ) filing a mold with glass microparticles, 2) filing the mold obtained in step 1 ) with a sol-gel solution comprising a compound selected among silicon dioxide, bore oxide, aluminium oxide and titanium oxide, thereby covering the glass microparticles, 3) gelling and drying at room temperature the sol-gel solution, thereby obtaining a glass material containing the glass microparticles, and 4) eventually removing the porous glass material containing the glass microparticles from the mold, thereby obtaining the glass macroporous matrix. The invention also relates to a process of cell culturing comprising the steps of: 1 ) preparing a glass macroporous matrix by the process as defined above, 2) applying cells at the surface of the matrix, 3) culturing the cells under culture conditions allowing the cells to colonize the matrix.

Abstract translation: 本发明涉及一种制备玻璃大孔基质的方法，其包括以下步骤：1）用玻璃微粒填充模具，2）将步骤1）中获得的模具用溶胶 - 凝胶 溶液，其包含选自二氧化硅，氧化钡，氧化铝和氧化钛的化合物，从而覆盖玻璃微粒; 3）在室温下凝胶化并干燥溶胶 - 凝胶溶液，从而获得包含玻璃微粒的玻璃材料;以及4 ）最终从模具中取出包含玻璃微粒的多孔玻璃材料，由此获得玻璃大孔基质。 本发明还涉及细胞培养方法，其包括以下步骤：1）通过如上定义的方法制备玻璃大孔基质，2）在细胞表面施用细胞，3）在培养条件下培养细胞，使得 细胞殖民矩阵。