公开(公告)号：WO2018034982A1

公开(公告)日：2018-02-22

申请号：PCT/US2017/046500

申请日：2017-08-11

Applicant: CBRITE INC.

Inventor： GUNNING, Kerry ,  ACKLEY, Donald ,  SHIEH, Chan-long

IPC: C12Q1/68 ,  C12M1/34

CPC classification number: G01N21/6428 ,  C12Q1/6869 ,  G01N27/4145 ,  G01N2021/6439 ,  G01N2201/062 ,  C12Q2563/107 ,  C12Q2565/607

Abstract: Apparatus for fluorescent and ion sensing of DNA nucleotide incorporation events including DNA nucleotide incorporation structure designed to have sequencing primers bonded to a surface for the incorporation of DNA nucleotides thereon. At least some of the DNA nucleotides having a fluorescent label. A photodiode positioned adjacent the incorporation structure and an illumination device positioned adjacent the DNA nucleotide incorporation structure to illuminate DNA nucleotides incorporated onto the sequencing primers. The illumination device exciting the fluorescent labels when incorporation occurs and the photodiode positioned to sense the excited fluorescent labels. Ion sensing apparatus positioned adjacent the DNA nucleotide incorporation structure including a metal oxide thin film transistor with a gate electrically coupled to receive an electrical signal indicative of ion emissions produced by the DNA nucleotide incorporated onto DNA target fragments or sequencing primers.

Abstract translation: 用于DNA核苷酸掺入事件的荧光和离子感应的装置，包括DNA核苷酸掺入结构，其被设计为具有结合到表面以将DNA核苷酸掺入其上的测序引物。 至少一些具有荧光标记的DNA核苷酸。 位于掺入结构附近的光电二极管和位于DNA核苷酸掺入结构附近的照明装置，以照射掺入到测序引物中的DNA核苷酸。 照明装置在结合时激发荧光标签并且光电二极管定位为感测激发的荧光标签。 离子感测装置位于DNA核苷酸掺入结构附近，包括金属氧化物薄膜晶体管，其栅极电耦合以接收指示由掺入DNA靶片段或测序引物的DNA核苷酸产生的离子发射的电信号。