公开(公告)号：WO1996041183A1

公开(公告)日：1996-12-19

申请号：PCT/US1996008758

申请日：1996-06-05

Applicant: CEDARS-SINAI MEDICAL CENTER

Inventor： CEDARS-SINAI MEDICAL CENTER ,  TARGAN, Stephan ,  VIDRICH, Alda

IPC: G01N33/563

CPC classification number: G01N33/56972 ,  C07K16/18 ,  G01N33/564

Abstract: The invention is directed to methods and kits for detecting and measuring the presence or absence of perinuclear anti-neutrophil cytoplasmic antibody of ulcerative colitis, primary sclerosing cholangitis or type 1 autoimmune hepatitis. The methods and kits of the present invention provide safe and reliable means for diagnosing ulcerative colitis, primary sclerosing cholangitis, and type 1 autoimmune hepatitis. The antigens reactive with perinuclear anti-neutrophil cytoplasmic autoantibody of ulcerative colitis and primary sclerosing cholangitis are also provided.

Abstract translation: 本发明涉及用于检测和测量溃疡性结肠炎，原发性硬化性胆管炎或1型自身免疫性肝炎的核周抗中性粒细胞胞质抗体的存在或不存在的方法和试剂盒。 本发明的方法和试剂盒提供用于诊断溃疡性结肠炎，原发性硬化性胆管炎和1型自身免疫性肝炎的安全可靠的手段。 还提供了与溃疡性结肠炎和原发性硬化性胆管炎的核周抗中性粒细胞胞质自身抗体反应的抗原。

公开(公告)号：WO1997000888A1

公开(公告)日：1997-01-09

申请号：PCT/US1996009691

申请日：1996-06-07

Applicant: CEDARS-SINAI MEDICAL CENTER

Inventor： CEDARS-SINAI MEDICAL CENTER ,  CUSHING, Susan ,  VIDRICH, Alda

IPC: C07K01/22

CPC classification number: C07K16/40 ,  C07K1/22 ,  C07K16/065 ,  Y10S435/962 ,  Y10S436/825

Abstract: In accordance with the present invention, there are provided novel methods and reagents for reducing background binding in antibody preparations having an unwanted affinity for intracellular protein(s). The invention method comprises treating an antibody preparation with permeabilized cells, then separating the antibody preparation from the permeabilized cells. The reagents are useful for reducing the level of background binding in antibody preparations. Also provided are antibody preparations that are substantially free of background binding.

Abstract translation: 根据本发明，提供了用于减少对细胞内蛋白质具有不希望的亲和力的抗体制剂中背景结合的新方法和试剂。 本发明方法包括用透化细胞处理抗体制剂，然后将抗体制剂与透化细胞分离。 这些试剂可用于降低抗体制剂中背景结合的水平。 还提供了基本上不含背景结合的抗体制剂。