公开(公告)号：WO1996040736A1

公开(公告)日：1996-12-19

申请号：PCT/US1996008113

申请日：1996-05-30

Applicant: CELTRIX PHARMACEUTICALS, INC.

Inventor： CELTRIX PHARMACEUTICALS, INC. ,  SOMMER, Andreas ,  OGAWA, Yasushi ,  TAO, Peggy

IPC: C07K01/14

CPC classification number: C07K1/1133 ,  C07K14/4743 ,  C07K14/65

Abstract: The present invention provides a novel method for refolding insulin-like growth factor I (IGF-I) and insulin-like growth factor binding protein 3 (IGFBP-3). The method involves mixing of IGF-I and IGFBP-3 together in a cofolding reaction. The inventive cofolding method results in substantially higher yields of correctly folded protein for both molecules and alters the kinetics of refolding. The method includes the production of correctly folded IGF-I, IGFBP-3, and/or IGF-I/IGFBP-3 complex.

Abstract translation: 本发明提供了一种用于重折叠胰岛素样生长因子I（IGF-I）和胰岛素样生长因子结合蛋白3（IGFBP-3）的新方法。 该方法包括在共转化反应中将IGF-1和IGFBP-3混合在一起。 本发明的共轭方法导致两分子的正确折叠的蛋白质的产量显着更高，并且改变重折叠的动力学。 该方法包括产生正确折叠的IGF-I，IGFBP-3和/或IGF-I / IGFBP-3复合物。