公开(公告)号：WO1993021207A1

公开(公告)日：1993-10-28

申请号：PCT/US1993003531

申请日：1993-04-14

Applicant: ABBOTT LABORATORIES

Inventor： ABBOTT LABORATORIES ,  CHU, Alexander, H.

IPC: C07K01/14

CPC classification number: C07K9/008

Abstract: A process for the manufacture of vancomycin which does not require preparation of a phosphate intermediate. The process consists of passing a vancomycin broth through a suitable adsorbent, followed by passing the vancomycin through a second adsorbent, producing a purified vancomycin. Purified vancomycin is then crystallized from the solution by adding a base solution that imparts a pH of above about 9.0 to about 9.5 to the purified vancomycin. The crystallized vancomycin is separated from the solution, dissolved in solution and recrystallized by adding a base solution which imparts a pH of above about 9.0 to about 9.5 to the dissolved solution. The recrystallized vancomycin is dissolved and titrated with hydrochloric acid. Vancomycin .HCl is then precipitated from the solution using an organic solvent.

Abstract translation: 一种不需要制备磷酸盐中间体的万古霉素的制造方法。 该方法包括将万古霉素肉汤通过合适的吸附剂，然后使万古霉素通过第二吸附剂，产生纯化的万古霉素。 纯化的万古霉素然后通过向纯化的万古霉素中加入提供高于约9.0-约9.5的pH的碱溶液从溶液中结晶。 将结晶的万古霉素与溶液分离，溶解在溶液中并通过加入向溶解溶液中赋予pH大于约9.0至约9.5的碱溶液重结晶。 将重结晶的万古霉素溶解并用盐酸滴定。 然后使用有机溶剂从溶液中沉淀万古霉素.HCl。

公开(公告)号：WO1990000177A1

公开(公告)日：1990-01-11

申请号：PCT/BR1989000004

申请日：1989-03-02

Applicant: BIOBRAS BIOQUIMICA DO BRASIL S.A. ,  MARES GUIA, Marcos, Luiz Dos ,  VILELA, Luciano ,  SANTOS PEREIRA XAVIER, Mariza, Suzana ,  RIBEIRO TUNES DE SOUZA, Heloisa ,  ZUCULIN JUNIOR, Marcelo ,  BAETA LAGE DE OLIVEIRA, Simone

Inventor： BIOBRAS BIOQUIMICA DO BRASIL S.A.

IPC: C07K01/14

CPC classification number: C07K1/18

Abstract: It is described an improved process for the large scale production of highly purified insulin crystals using at the ion exchange chromatography of the insulin gels of a high chemical and physical stability and thus achieving an increase of flow rate and of the productivity, defined as the amount of insulin processed per gel volume per day.

Abstract translation: 描述了使用在高化学和物理稳定性的胰岛素凝胶的离子交换层析下大规模生产高纯度胰岛素晶体的改进方法，从而实现了流速和生产率的增加，定义为量 的每天凝胶体积加工的胰岛素。

公开(公告)号：WO1998015570A1

公开(公告)日：1998-04-16

申请号：PCT/US1997018222

申请日：1997-10-09

Applicant: SYNAPTIC PHARMACEUTICAL CORPORATION ,  BARD, Jonathan, A. ,  BOROWSKY, Beth ,  SMITH, Kelli, E. ,  BRANCHEK, Theresa, A. ,  GERALD, Christophe, P., G. ,  JONES, Kenneth, A.

Inventor： SYNAPTIC PHARMACEUTICAL CORPORATION

IPC: C07K01/14

CPC classification number: C07K16/2869 ,  A01K2217/05 ,  A61K38/00 ,  C07K14/72 ,  G01N33/5088 ,  G01N33/74 ,  G01N2333/72 ,  G01N2500/10

Abstract: This invention provides an isolated nucleic acid encoding a mammalian galanin receptor, an isolated galanin receptor protein, vectors comprising isolated nucleic acid encoding a mammalian galanin receptor, cells comprising such vectors, antibodies directed to a mammalian galanin receptor, nucleic acid probes useful for detecting nucleic acid encoding a mammalian galanin receptor, antisense oligonucleotides complementary to unique sequences of nucleic acid encoding a mammalian galanin receptor, nonhuman transgenic animals which express DNA encoding a normal or a mutant mammalian galanin receptor, as well as methods of determining binding of compounds to mammalian galanin receptors.

Abstract translation: 本发明提供编码哺乳动物甘丙肽受体的分离的核酸，分离的甘丙肽受体蛋白，包含编码哺乳动物甘丙肽受体的分离的核酸的载体，包含该载体的细胞，针对哺乳动物甘丙肽受体的抗体，用于检测核酸的核酸探针 编码哺乳动物甘丙肽受体的酸，与编码哺乳动物甘丙肽受体的核酸的独特序列互补的反义寡核苷酸，表达编码正常或突变型哺乳动物甘丙肽受体的DNA的非人转基因动物，以及确定化合物与哺乳动物甘丙肽的结合的方法 受体。

公开(公告)号：WO1995024418A1

公开(公告)日：1995-09-14

申请号：PCT/US1995001593

申请日：1995-02-02

Applicant: MINNESOTA MINING AND MANUFACTURING COMPANY

Inventor： MINNESOTA MINING AND MANUFACTURING COMPANY ,  HEILMANN, Steven, M. ,  DRTINA, Gary, J. ,  EITZMAN, Philip, D. ,  HADDAD, Louis, C. ,  HYDE, Frederick, W. ,  JOHNSON, Todd, W. ,  RASMUSSEN, Jerald, K. ,  WILLIAMS, Michael, G.

IPC: C07K01/14

CPC classification number: B01D39/083 ,  B01D15/08 ,  B01D15/361 ,  B01D15/3804 ,  B01D39/086 ,  B01D39/1623 ,  B01D39/18 ,  B01D39/2068 ,  B01D2239/0407 ,  B01D2239/0428 ,  B01D2239/1291 ,  C07K1/34

Abstract: The invention provides a method of separating a biomacromolecule which comprises the steps of providing a separation system including a filter element which comprises a composite filtration medium, said composite filtration medium comprising a filtration layer on the upstream surface of which are located insoluble stationary phase particulates, the particulates being capable of binding to a biomacromolecule or class of biomacromolecules, a reservoir containing a solution mixture comprising at least one biomacromolecule as solute, and a pump and associated tubing to form a closed loop assembly, and recirculation pumping the solution mixture through the filter cartridge so as to bind the at least one biomacromolecule to the stationary phase particulate so as to form a biomacromolecule:stationary phase particulate product. An eluting solution can be pumped through the closed loop assembly which is capable of reversing the biomacromolecule:stationary phase particulate product binding interaction so as to liberate the biomacromolecule.

Abstract translation: 本发明提供了分离生物大分子的方法，其包括提供包括复合过滤介质的过滤元件的分离系统的步骤，所述复合过滤介质在其上游表面上设置有不溶性固定相颗粒的过滤层， 该微粒能够结合生物大分子或一类生物大分子，含有包含至少一种生物大分子作为溶质的溶液混合物的储存器，以及泵和相关管道以形成闭环组件，以及再循环泵送溶液混合物通过过滤器 以便将至少一种生物大分子结合至固定相颗粒，以便形成生物大分子：固定相颗粒产物。 洗脱溶液可以泵送通过闭环组件，其能够逆转生物大分子：固定相颗粒产物结合相互作用以释放生物大分子。

公开(公告)号：WO1995022555A1

公开(公告)日：1995-08-24

申请号：PCT/US1995001966

申请日：1995-02-16

Applicant: CRAMER, Steven, M. ,  MOORE, James, A.

Inventor： KUNDU, Amitava ,  LI, Yufei ,  JAYARAMAN, Guhan

IPC: C07K01/14

CPC classification number: B01D15/422 ,  C07C215/40 ,  C07K1/18 ,  G01N30/02

Abstract: A method for the purification of proteins by displacement chromatography on ion exchange media using molecular weight displacers is disclosed. Several classes of low molecular weight, charged species are exemplified, including aminoacids, peptides, antibiotics and dendrimeric polymers. Novel compounds useful as displacers are dendrimers of formula (I), wherein R is lower alkyl, n is 2 to 6, and X is a common counter anion and similar dendritic polymers based thereon.

Abstract translation: 公开了一种使用分子量置换器在离子交换介质上通过置换色谱法纯化蛋白质的方法。 例举了几类低分子量带电物质，包括氨基酸，肽，抗生素和树枝状聚合物。 用作置换剂的新型化合物是式（I）的树枝状大分子，其中R 1是低级烷基，n是2-6，X是常见的抗衡阴离子和基于其的类似树枝状聚合物。

公开(公告)号：WO1995008562A1

公开(公告)日：1995-03-30

申请号：PCT/AU1994000562

申请日：1994-09-20

Applicant: ANADIS LTD. ,  GRAHAM, Conor, John

Inventor： ANADIS LTD.

IPC: C07K01/14

CPC classification number: C07K16/1232 ,  A61K9/2004 ,  A61K9/2846 ,  C07K16/04 ,  Y02A50/472 ,  Y10S424/809

Abstract: A method of obtaining a high purity immunoglobulin preparation from an antibody rich colostrum which includes: (i) removing milk fat from the colostrum to obtain a low-fat colostrum; (ii) pasteurising the low-fat colostrum; (iii) coagulating the pasteurised, low-fat colostrum and removing milk curd containing casein; (iv) centrifuging remaining liquid to remove percipitates; (v) removing lactose, minerals and water to obtain an antibody containing fraction; (vi) dissolving the antibody containing fraction in THRESH buffer and idolizing against the same buffer; and (vii) concentrating the antibody containing solution to obtain a 10 % by weight antibody solution. A pharmaceutical composition including a core element which includes an active antibody component derived by the above method, wherein the core element is in the form of a tablet, and wherein the compression forces used to prepare the tablet are such that they do not injure or denature the active antibodies.

Abstract translation: 从富含抗体的初乳获得高纯度免疫球蛋白制剂的方法，其包括：（i）从初乳中除去乳脂以获得低脂肪初乳; （ii）巴氏杀菌低脂初乳; （iii）使巴氏灭菌的低脂初乳凝结并除去含有酪蛋白的牛奶凝乳; （iv）离心剩余液体以除去percipitates; （v）除去乳糖，矿物质和水，得到含抗体的级分; （vi）将含抗体的级分溶解于THRESH缓冲液中，并与同样的缓冲液偶像; 和（vii）浓缩含抗体的溶液以获得10重量％的抗体溶液。 一种药物组合物，其包含核心元件，其包含通过上述方法衍生的活性抗体成分，其中所述核心元件为片剂形式，并且其中用于制备片剂的压缩力使得它们不会损伤或变性 活性抗体。

公开(公告)号：WO1997018234A2

公开(公告)日：1997-05-22

申请号：PCT/US1996018168

申请日：1996-11-13

Applicant: GENENCOR INTERNATIONAL, INC.

Inventor： GENENCOR INTERNATIONAL, INC. ,  BECKMAN, Eric, J. ,  GHENCIU, Eliador, G. ,  BECKER, Nathaniel, T. ,  STEELE, Landon, M.

IPC: C07K01/14

CPC classification number: C12N9/54 ,  C07K1/145 ,  C07K14/465 ,  Y02P20/544 ,  Y10S435/814

Abstract: There is provided a process for the extraction of water soluble biomaterials such as enzymes or proteins into carbon dioxide utilizing certain carbon dioxide-soluble surfactants. Also provided are certain carbon dioxide-soluble surfactants useful in the extraction of proteins.

Abstract translation: 提供了利用某些二氧化碳可溶性表面活性剂将水溶性生物材料如酶或蛋白质提取到二氧化碳中的方法。 还提供了可用于提取蛋白质的某些二氧化碳可溶性表面活性剂。

公开(公告)号：WO1996040736A1

公开(公告)日：1996-12-19

申请号：PCT/US1996008113

申请日：1996-05-30

Applicant: CELTRIX PHARMACEUTICALS, INC.

Inventor： CELTRIX PHARMACEUTICALS, INC. ,  SOMMER, Andreas ,  OGAWA, Yasushi ,  TAO, Peggy

IPC: C07K01/14

CPC classification number: C07K1/1133 ,  C07K14/4743 ,  C07K14/65

Abstract: The present invention provides a novel method for refolding insulin-like growth factor I (IGF-I) and insulin-like growth factor binding protein 3 (IGFBP-3). The method involves mixing of IGF-I and IGFBP-3 together in a cofolding reaction. The inventive cofolding method results in substantially higher yields of correctly folded protein for both molecules and alters the kinetics of refolding. The method includes the production of correctly folded IGF-I, IGFBP-3, and/or IGF-I/IGFBP-3 complex.

Abstract translation: 本发明提供了一种用于重折叠胰岛素样生长因子I（IGF-I）和胰岛素样生长因子结合蛋白3（IGFBP-3）的新方法。 该方法包括在共转化反应中将IGF-1和IGFBP-3混合在一起。 本发明的共轭方法导致两分子的正确折叠的蛋白质的产量显着更高，并且改变重折叠的动力学。 该方法包括产生正确折叠的IGF-I，IGFBP-3和/或IGF-I / IGFBP-3复合物。

公开(公告)号：WO1996032406A1

公开(公告)日：1996-10-17

申请号：PCT/US1996005186

申请日：1996-04-15

Applicant: BIOELASTICS RESEARCH LTD. ,  UAB RESEARCH FOUNDATION

Inventor： BIOELASTICS RESEARCH LTD. ,  UAB RESEARCH FOUNDATION ,  URRY, Dan, W ,  McPHERSON, David, T. ,  XU, Jie

IPC: C07K01/14

CPC classification number: C07K14/78 ,  C07K14/001

Abstract: A method for purifying an artificial polymer that exhibits a reversible inverse temperature transition is provided. The method involves (a) dissolving the polymer in an aqueous medium so that the temperature of the medium is below the effective transition temperature; (b) adjusting the temperature of the aqueous medium relative to the effective transition temperature of the polymer; (c) removing any particulate material from the medium; (d) adjusting the temperature of the aqueous medium relative to the effective transition temperature of the polymer so that the temperature of the medium is above the effective transition temperature; (e) collecting the polymer from the medium as a more dense phase; and (f) optionally repeating any of steps (a)-(e) until a desired level of purity is reached; with the proviso the order of steps can be (a)-(d)-(e)-(a)-(b)-(c).

Abstract translation: 提供了一种纯化呈现出可逆逆温转变的人造聚合物的方法。 该方法包括（a）将聚合物溶解在水性介质中，使得介质的温度低于有效转变温度; （b）相对于聚合物的有效转变温度调节水性介质的温度; （c）从介质中除去任何颗粒物质; （d）相对于聚合物的有效转变温度调节水性介质的温度，使得介质的温度高于有效转变温度; （e）从介质中收集聚合物作为更致密的相; 和（f）任选地重复步骤（a） - （e）中的任何步骤，直到达到所需的纯度水平; 条件是步骤的顺序可以是（a） - （d） - （e） - （a） - （b） - （c）。

公开(公告)号：WO1994025480A1

公开(公告)日：1994-11-10

申请号：PCT/JP1994000731

申请日：1994-04-28

Applicant: TOKYO TANABE COMPANY LIMITED ,  TAKEI, Tsunetomo ,  AIBA, Toshimitsu ,  SAKAI, Kaoru ,  FUJIWARA, Tetsuro

Inventor： TOKYO TANABE COMPANY LIMITED

IPC: C07K01/14

CPC classification number: C07K14/785

Abstract: A method of purifying a hydrophobic polypeptide by high-performance liquid chromatography using a mobile phase comprising a solvent mixture wherein the content of trifluoroacetic acid is 3-10 % (v/v) and a packing material comprising polyvinyl alcohol. This method is useful because a lung surfactant prepared from the hydrophobic polypeptide purified by this method has a surface activity superior to that of the hydrophobic polypeptide purified by the conventional methods.

Abstract translation: 一种通过高效液相色谱法使用包含三氟乙酸含量为3-10％（v / v）的溶剂混合物的流动相和包含聚乙烯醇的包装材料的方法纯化疏水多肽的方法。 该方法是有用的，因为通过该方法纯化的疏水性多肽制备的肺表面活性比通过常规方法纯化的疏水多肽的表面活性更好。