公开(公告)号：WO2019060629A1

公开(公告)日：2019-03-28

申请号：PCT/US2018/052074

申请日：2018-09-20

Applicant: CODIAK BIOSCIENCES, INC.

Inventor： VILLIGER, Agata A. ,  GRUBE, Andrew F. ,  CHAN, Tik Yan ,  ESTES, Scott D. ,  GOLDEN, Kathryn E. ,  KONSTANTINOV, Konstantin ,  WILLIAMS, Douglas E.

IPC: A61K35/12 ,  A61K35/14 ,  A61K35/28 ,  A61K35/33 ,  A61K35/35 ,  C12N5/07

CPC classification number: C12N5/0043 ,  C12N2500/92 ,  C12P1/00

Abstract: Described herein are methods for the production of extracellular vesicles comprising culturing a population of producer cells in single-cell suspension, wherein the cells are cultured in chemically-defined culture medium, wherein the culture medium lacks animal- derived serum and animal-derived components; and obtaining from the cell culture an extracellular vesicle preparation comprising extracellular vesicles. In certain embodiments, the methods comprise perfusion culturing methods, including single-cell perfusion culturing methods and batch-refeed culturing methods. The methods described herein are a significant improvement over the state of the art and fulfills an unmet need in the field of extracellular vesicle manufacturing and quality control.