公开(公告)号：WO2022066934A2

公开(公告)日：2022-03-31

申请号：PCT/US2021/051783

申请日：2021-09-23

Applicant: CODIAK BIOSCIENCES, INC.

Inventor： BOURDEAU, Raymond ,  DOHERTY, Michael F. ,  NOYES, Aaron ,  DESANTY, Kayla

IPC: B01D15/16 ,  B01D15/20 ,  C12N15/10 ,  A61K35/12 ,  C12N5/00 ,  B01D15/166 ,  B01D15/203 ,  C12N15/101

Abstract: The present disclosure relates to multistep chromatographic methods for preparing extracellular vesicles (EVs). The methods were demonstrated to be effective in preparing highquality EVs in a large scale. The methods enable preparation of EVs for therapeutic and diagnostic applications, and isolation and/or sub-fractionation of EVs with desired properties for specific use.

公开(公告)号：WO2022066898A2

公开(公告)日：2022-03-31

申请号：PCT/US2021/051742

申请日：2021-09-23

Applicant: CODIAK BIOSCIENCES, INC.

Inventor： SOOS, Tim ,  NOYES, Aaron ,  DOOLEY, Kevin P. ,  ZHANG, Eric

IPC: A61K39/12 ,  A61K39/215 ,  A61P11/00 ,  A61P31/14 ,  A61K2039/60 ,  A61K2039/62 ,  A61K2039/625 ,  A61K2039/64 ,  C12N2770/20011 ,  C12N2770/20034

Abstract: The present disclosure relates to extracellular vesicles (EVs), e.g., exosomes, which can be rapidly modified to comprise an antigen of interest, and the uses of such EVs as vaccines for the treatment of wide range of diseases or disorders. Also provided herein are methods for preparing and manufacturing such EVs for use as vaccines.

公开(公告)号：WO2021184021A1

公开(公告)日：2021-09-16

申请号：PCT/US2021/022433

申请日：2021-03-15

Applicant: CODIAK BIOSCIENCES, INC.

Inventor： BROOM, Wendy ,  XU, Ke ,  ZHOU, Jing ,  PATEL, Shailendra ,  ZHANG, Wei ,  SATHYANARAYAN, Sriram

IPC: C12N15/113 ,  A61K31/7125 ,  A61K9/127 ,  A61P25/00

Abstract: The present disclosure relates to extracellular vesicles, e.g., exosomes, comprising an antisense oligonucleotide (ASO), wherein the ASO comprises a contiguous nucleotide sequence of 10 to 30 nucleotides in length that is complementary to a nucleic acid sequence within a peripheral myelin protein 22 (PMP22) transcript. Also provided herein are methods for producing the exosomes and methods for using the exosomes to treat and/or prevent diseases or disorders.

公开(公告)号：WO2021062290A1

公开(公告)日：2021-04-01

申请号：PCT/US2020/052901

申请日：2020-09-25

Applicant: CODIAK BIOSCIENCES, INC.

Inventor： BOURDEAU, Raymond ,  JANG, Su Chul ,  HARRISON, Rane ,  O'NEIL, Conlin ,  NOYES, Aaron

IPC: B01D15/38 ,  B01D15/36

Abstract: Provided herein are methods of preparing EVs, e.g., exosomes, associated with or encapsulated various cyclic dinucleotides, including STING agonists. Also provided herein are methods of loading EVs, e.g., exosomes, with various cyclic dinucleotides, including STING agonists.

公开(公告)号：WO2021062057A1

公开(公告)日：2021-04-01

申请号：PCT/US2020/052583

申请日：2020-09-24

Applicant: CODIAK BIOSCIENCES, INC.

Inventor： GOLDEN, Kathryn E. ,  DOHERTY, Michael F.

IPC: A61K9/127 ,  A61K9/19 ,  A61K9/50

Abstract: Methods of loading vesicles (e.g., exosomes) with exogenous cargo, compositions, reaction mixtures, and methods of treating a disease are disclosed herein.

公开(公告)号：WO2020191361A2

公开(公告)日：2020-09-24

申请号：PCT/US2020/024023

申请日：2020-03-20

Applicant: CODIAK BIOSCIENCES, INC.

Inventor： MONIZ, Raymond J. ,  MCCONELL, Russell E. ,  ROSS, Nikki ,  MCCOY, Christine ,  SOOS, Timothy J. ,  XU, Ke

IPC: C07K14/705 ,  C12N15/62 ,  A61K39/39 ,  A61K9/127 ,  A61K31/7084 ,  A61K39/00 ,  A61K47/69 ,  A61K45/00 ,  A61P31/00 ,  A61P33/02 ,  A61P35/00 ,  A61P31/12

Abstract: The present disclosure relates to extracellular vesicles (EVs), e.g., exosomes, comprising a payload (e.g., an antigen, adjuvant, and/or immune modulator) and/or a targeting moiety. Also provided herein are methods for producing the EVs (e.g., exosomes) and methods for using the EVs (e.g., exosomes) to treat and/or prevent diseases or disorders, e.g., cancer, graft-versus-host disease (GvHD), autoimmune disease, infectious diseases, or fibrotic diseases.

公开(公告)号：WO2019157535A1

公开(公告)日：2019-08-15

申请号：PCT/US2019/017731

申请日：2019-02-12

Applicant: CODIAK BIOSCIENCES, INC.

Inventor： SATHYANARAYANAN, Sriram ,  WILLIAMS, Douglas, E. ,  BURZYN, Dalia ,  BOUTIN, Adam, Thomas ,  KAMERKAR, Sushrut

IPC: C12N15/113 ,  A61K31/7088 ,  A61P35/00

Abstract: Disclosed herein are compositions and methods comprising extracellular vesicles comprising nucleic acid that target genes, leading to macrophage polarization of tumor associated macrophages. In certain embodiments, disclosed herein are methods and compositions for increasing macrophage polarization for the treatment of cancer.

公开(公告)号：WO2019060629A1

公开(公告)日：2019-03-28

申请号：PCT/US2018/052074

申请日：2018-09-20

Applicant: CODIAK BIOSCIENCES, INC.

Inventor： VILLIGER, Agata A. ,  GRUBE, Andrew F. ,  CHAN, Tik Yan ,  ESTES, Scott D. ,  GOLDEN, Kathryn E. ,  KONSTANTINOV, Konstantin ,  WILLIAMS, Douglas E.

IPC: A61K35/12 ,  A61K35/14 ,  A61K35/28 ,  A61K35/33 ,  A61K35/35 ,  C12N5/07

CPC classification number: C12N5/0043 ,  C12N2500/92 ,  C12P1/00

Abstract: Described herein are methods for the production of extracellular vesicles comprising culturing a population of producer cells in single-cell suspension, wherein the cells are cultured in chemically-defined culture medium, wherein the culture medium lacks animal- derived serum and animal-derived components; and obtaining from the cell culture an extracellular vesicle preparation comprising extracellular vesicles. In certain embodiments, the methods comprise perfusion culturing methods, including single-cell perfusion culturing methods and batch-refeed culturing methods. The methods described herein are a significant improvement over the state of the art and fulfills an unmet need in the field of extracellular vesicle manufacturing and quality control.

公开(公告)号：WO2021184020A1

公开(公告)日：2021-09-16

申请号：PCT/US2021/022428

申请日：2021-03-15

Applicant: CODIAK BIOSCIENCES, INC.

Inventor： LIM, Joanne ,  KIRWIN, Katherine ,  BROOM, Wendy ,  SATHYANARAYANAN, Sriram ,  VERMA, Ajay ,  ROSS, Nikki ,  JANG, Su Chul ,  ECONOMIDES, Kyriakos

IPC: A61P25/00 ,  A61P25/28 ,  A61K35/12 ,  A61K31/7088 ,  A61K38/16 ,  A61K38/10 ,  A61K38/08 ,  A61K31/712 ,  A61K31/711 ,  A61K47/30

Abstract: The present disclosure relates to extracellular vesicles, e.g., exosomes, comprising an M2 polarization agent. In some aspects, the M2 polarization agent comprises an antisense oligonucleotide (ASO). Also provided herein are methods for producing the exosomes and methods for using the exosomes to treat and/or prevent diseases or disorders.

公开(公告)号：WO2021184017A1

公开(公告)日：2021-09-16

申请号：PCT/US2021/022420

申请日：2021-03-15

Applicant: CODIAK BIOSCIENCES, INC.

Inventor： VERMA, Ajay ,  SOOS, Tim ,  ROSS, Nikki ,  MCCOY, Christine ,  FINN, Jonathan ,  SATHYANARAYANAN, Sriram ,  PATEL, Shailendra ,  XU, Ke ,  MCCONNELL, Russell E. ,  DOOLEY, Kevin P.

IPC: A61K39/00 ,  A61P25/28 ,  C07K17/02

Abstract: The present disclosure relates to extracellular vesicles (EVs) that are capable of targeting a cell in the CNS of a subject. Also provided herein are methods for producing the EVs and methods for using the EVs to treat and/or prevent diseases or disorders of the CNS (e.g., neurological disorders).