公开(公告)号：WO2020011847A1

公开(公告)日：2020-01-16

申请号：PCT/EP2019/068519

申请日：2019-07-10

Applicant: CONSEJO SUPERIOR DE INVESTIGACIONES CIENTÍFICAS

Inventor： FERNÁNDEZ FUEYO, Elena ,  ARANDA OLIDEN, Carmen ,  GUTIÉRREZ SUÁREZ, Ana ,  MARTÍNEZ FERRER, Angel T.

IPC: C12N9/08 ,  C07K14/375 ,  C12N15/52 ,  C12N15/63 ,  C12N15/70 ,  C12N15/72 ,  C12N15/74 ,  C12P7/00

Abstract: The present invention relates to a method for heterologous expression of active fungal unspecific peroxygenase and/or variants thereof in bacterial host cells, preferably in Escherichia coli . The invention also relates to the recombinant active fungal unspecific peroxygenase and/or variants thereof thus obtained and to the use thereof. The invention further relates to a method for epoxidation of unsaturated fatty acids, or oxyfunctionalization of other organic compounds, comprising the recombinant active fungal unspecific peroxygenase and/or variants thereof obtainable by the method disclosed herein.