公开(公告)号：WO1997022878A2

公开(公告)日：1997-06-26

申请号：PCT/US1996019560

申请日：1996-12-12

Applicant: COULTER INTERNATIONAL CORP.

Inventor： COULTER INTERNATIONAL CORP. ,  LI, Yi ,  YOUNG, Carole ,  VEULENS, Sergio, C.

IPC: G01N33/50

CPC classification number: G01N33/5094 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/25125

Abstract: This invention relates to a lytic reagent and a method of using the lytic reagent for automatically determining leukocyte subpopulations in blood. More specifically, the new lytic reagent lyses red blood cells and affects the eosinophils which enables the differentiation of at least one subpopulation of leukocytes. The lytic reagent is an acidic, hypertonic aqueous solution comprising an alkali metal salt of alkyl sulfate, an eosinolytic agent, a nonionic surfactant and a physiological salt. When used in combination with a second lytic reagent system, one is able to obtain at least a five part differential of leukocytes using DC and RF measurements.

Abstract translation: 本发明涉及溶解试剂和使用溶解剂自动测定血液中白细胞亚群的方法。 更具体地，新的溶解试剂溶解红细胞并影响嗜酸性粒细胞，其能够分化白细胞的至少一个亚群。 裂解试剂是含有烷基硫酸盐的碱金属盐，溶解剂，非离子表面活性剂和生理盐的酸性高渗水溶液。 当与第二溶解试剂系统组合使用时，可以使用DC和RF测量获得白细胞的至少五分之一的差异。