公开(公告)号：WO2003012426A1

公开(公告)日：2003-02-13

申请号：PCT/US2002/022414

申请日：2002-07-15

Applicant: COULTER INTERNATIONAL CORP.

Inventor： LI, Yi ,  LI, Jing

IPC: G01N31/00

CPC classification number: G01N33/80 ,  G01N15/12 ,  G01N15/1459 ,  G01N33/5002 ,  G01N33/721 ,  G01N2015/1006 ,  G01N2015/1493 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/108331

Abstract: A lytic reagent composition for measuring nucleated blood cells in a blood sample is described. The lytic reagent composition comprises a quaternary ammonium surfactant, an ethoxlyated phenol, and an ethoxylated alcohol. When mixed with a blood sample, the lytic reagent composition lyses red blood cells and enables a differentiation of nucleated red blood cells from other cell types by DC impedance measurement. The lytic reagent composition can further comprise an organic ligand for determining total hemoglobin concentration of a blood sample photometrically. Further disclosed is a lytic reagent system including the lytic reagent composition and a diluent. In addition, a single reagent composition containing salts is also disclosed, which can be used without a separate diluent. The lytic reagent compositions can be used for concurrent measurement of nucleated red blood cells, WBC, and hemoglobin of a blood sample.

Abstract translation: 描述了用于测量血液样品中的有核血细胞的溶解试剂组合物。 裂解试剂组合物包含季铵表面活性剂，乙氧基化苯酚和乙氧基化醇。 当与血液样品混合时，溶解试剂组合物溶解红细胞，并且通过DC阻抗测量使得有核红细胞与其它细胞类型分化。 溶解试剂组合物可进一步包含用于测定血样的总血红蛋白浓度的有机配体。 还公开了包括溶解试剂组合物和稀释剂的裂解试剂系统。 此外，还公开了含有盐的单一试剂组合物，其可在没有单独的稀释剂的情况下使用。 裂解试剂组合物可用于同时测量血液样品的有核红细胞，WBC和血红蛋白。

公开(公告)号：WO2002085506A1

公开(公告)日：2002-10-31

申请号：PCT/US2002/011193

申请日：2002-04-03

Applicant: COULTER INTERNATIONAL CORP.

Inventor： HUSHER, Frederick, K.

IPC: B01F15/02

CPC classification number: G01N15/1404 ,  B01F3/088 ,  B01F15/00019 ,  B01F15/0022 ,  B01F15/0408 ,  B01F15/0437 ,  B01F2003/0896 ,  B01F2215/0034 ,  G01F23/74 ,  G01N1/38 ,  G01N2015/1006 ,  G01N2015/1409 ,  G05D11/138

Abstract: A physically compact (saline) solution preparation apparatus includes a solution mixing tank (10) coupled to sources of concentrate and diluent. A concentrate transport path (16) is configured to both homogenize and deliver concentrate to the mixing tank, while the diluent transport path (12) is controllably purged of potential contaminates prior to supplying the diluent to the mixing tank. A mixed solution transport path (16) recirculates and thereby homogenizes the contents of the mixing container, and actively pumps out solution that has been mixed to a prescribed conductivity-based concentration, to one or more solution on demand storage reservoirs (24) for respective instruments. The operation of the apparatus is based upon measured conductivity of the contents of the solution mixing tank, so that there is no need to conduct precursor volumetric measurements of either diluent or concentrate.

Abstract translation: 物理紧凑（盐水）溶液制备装置包括与浓缩物和稀释剂源连接的溶液混合罐（10）。 浓缩物输送路径（16）构造成均匀化并将浓缩物输送到混合罐，同时稀释剂输送路径（12）在将稀释剂供应到混合罐之前被可控地清除潜在的污染物。 混合溶液输送路径（16）再循环，从而使混合容器的内容物均化，并且主动将已经混合到规定的基于导电率的浓度的溶液泵送到一个或多个溶液按需储存容器（24），用于各自的 仪器。 该装置的操作基于溶液混合罐的内容物的测量电导率，使得不需要进行稀释剂或浓缩物的前体体积测量。

公开(公告)号：WO1998029726A2

公开(公告)日：1998-07-09

申请号：PCT/US1997023529

申请日：1997-12-18

Applicant: COULTER INTERNATIONAL CORP.

Inventor： COULTER INTERNATIONAL CORP. ,  THOMPSON, Stephan, G.

IPC: G01N00/00

CPC classification number: G01N33/6887 ,  G01N2333/4712 ,  G01N2800/324

Abstract: The present invention provides a method for detecting myocardial necrosis by incubating a sample with a natural binding partner (troponin C) for troponin I and antibodies to troponin C and troponin I; and determining the resultant immune complex formed in proportion to the total concentration of troponin I in the sample.

Abstract translation: 本发明提供一种通过将样品与肌钙蛋白I的天然结合配偶体（肌钙蛋白C）和肌钙蛋白C和肌钙蛋白I的抗体孵育来检测心肌坏死的方法; 并确定与样品中肌钙蛋白I的总浓度成比例地形成的所得免疫复合物。

公开(公告)号：WO1997022878A2

公开(公告)日：1997-06-26

申请号：PCT/US1996019560

申请日：1996-12-12

Applicant: COULTER INTERNATIONAL CORP.

Inventor： COULTER INTERNATIONAL CORP. ,  LI, Yi ,  YOUNG, Carole ,  VEULENS, Sergio, C.

IPC: G01N33/50

CPC classification number: G01N33/5094 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/25125

Abstract: This invention relates to a lytic reagent and a method of using the lytic reagent for automatically determining leukocyte subpopulations in blood. More specifically, the new lytic reagent lyses red blood cells and affects the eosinophils which enables the differentiation of at least one subpopulation of leukocytes. The lytic reagent is an acidic, hypertonic aqueous solution comprising an alkali metal salt of alkyl sulfate, an eosinolytic agent, a nonionic surfactant and a physiological salt. When used in combination with a second lytic reagent system, one is able to obtain at least a five part differential of leukocytes using DC and RF measurements.

Abstract translation: 本发明涉及溶解试剂和使用溶解剂自动测定血液中白细胞亚群的方法。 更具体地，新的溶解试剂溶解红细胞并影响嗜酸性粒细胞，其能够分化白细胞的至少一个亚群。 裂解试剂是含有烷基硫酸盐的碱金属盐，溶解剂，非离子表面活性剂和生理盐的酸性高渗水溶液。 当与第二溶解试剂系统组合使用时，可以使用DC和RF测量获得白细胞的至少五分之一的差异。

公开(公告)号：WO1997001757A1

公开(公告)日：1997-01-16

申请号：PCT/US1996011127

申请日：1996-06-28

Applicant: COULTER INTERNATIONAL CORP.

Inventor： COULTER INTERNATIONAL CORP. ,  LI, Yi ,  YOUNG, Carole ,  FISCHER, Timothy, J.

IPC: G01N33/50

CPC classification number: G01N33/5094 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/25125 ,  Y10T436/2525

Abstract: A lytic reagent composition is provided which selectively stromatolyses red blood cells in a blood sample. In addition, a lytic reagent system is provided which enables the differentiation of at least three subpopulations of leukocytes. A method for using the lytic reagent system is also provided. Still further, the lytic reagent system finds use in the determination of the hemoglobin in the blood. The lytic reagent system utilizes an alkyl sulfate, polyoxyethylene-based surfactant and acid with a hypertonic, alkaline, stabilizing reagent. The system and analysis method maintains the cellular morphology of the leukocytes and can be used to analyze normal and abnormal blood samples, fresh and aged blood, human and non-human animal blood samples.

Abstract translation: 提供溶解试剂组合物，其选择性地在血液样品中进行红细胞分解。 此外，提供了一种裂解试剂系统，其能够分化至少三个亚群的白细胞。 还提供了使用溶解试剂系统的方法。 此外，溶解试剂系统用于测定血液中的血红蛋白。 溶解试剂系统使用烷基硫酸盐，聚氧乙烯基表面活性剂和酸与高渗，碱性，稳定剂。 系统和分析方法维持白细胞的细胞形态，可用于分析正常和异常血液样本，新鲜和老化血液，人和非人动物血液样品。

公开(公告)号：WO1996030751A1

公开(公告)日：1996-10-03

申请号：PCT/US1996004113

申请日：1996-03-26

Applicant: COULTER INTERNATIONAL CORP.

Inventor： COULTER INTERNATIONAL CORP. ,  AZARNIA, Roobik ,  FUENTES, Susan ,  RUSSEL, Thomas

IPC: G01N27/28

CPC classification number: C12Q1/006 ,  C12Q1/00 ,  G01N27/3271 ,  Y10S435/817 ,  Y10T436/117497

Abstract: An apparatus (10) for measuring an analyte in a fluid sample (12) comprises a housing (H) defining a pair of chambers (26, 30) separated by a semipermeable membrane (28). The membrane allows the analyte of interest to pass between the chambers. The second chamber (30) contains a detector adapted to either (a) directly detect the analyte of interest or (b) to detect a reaction by product of the analyte upon reacting with a substance (14) such as an enzyme, introduced into the second chamber. Means are provided to flush the second chamber after each analyte measurement.

Abstract translation: 用于测量流体样品（12）中的分析物的装置（10）包括限定由半透膜（28）分离的一对腔室（26,30）的壳体（H）。 膜允许感兴趣的分析物在腔室之间通过。 第二室（30）包含检测器，其适于（a）直接检测感兴趣的分析物，或（b）通过与分析物的产物（例如酶）反应来检测反应物，所述物质（14）被引入到 第二室。 提供了在每次分析物测量之后冲洗第二室的装置。

公开(公告)号：WO2004019031A1

公开(公告)日：2004-03-04

申请号：PCT/US2003/024429

申请日：2003-08-06

Applicant: COULTER INTERNATIONAL CORP.

Inventor： KRAMER, Donald, L.

IPC: G01N31/00

CPC classification number: G01N15/1459 ,  G01N15/1031 ,  G01N2015/008 ,  G01N2015/0084 ,  G01N2015/1006 ,  G01N2015/1486 ,  G01N2015/1493 ,  Y10T436/10 ,  Y10T436/101666

Abstract: Blood cells of interest are readily distinguishable from other blood cells and look-a-like particles found in a blood sample by their back-scatter signature. A preferred method for differentiating platelets in a blood sample is to irradiate the cells and particles, one at a time, with a beam of radiation (B'), and to detect back-scattered (reflected) radiation (D') using a plurality of optical fibers (54) to transmit the back-scattered radiation (D') to a highgain photodetector (56), e.g. a photomultiplier tube. Preferably, the back scatter signal so obtained is combined with a second signal representing, for example, either the level of forward-scatter (FS) within a prescribed, relatively narrow angular range, or the level of side-scattered radiation (SS), or the level of attenuation of the cell-irradiating beam caused by the presence of the irradiated cell or particle in the beam, or the electrical impedance of the irradiated cell or particle, to differentiate the cells of interest. The method and apparatus of the invention are particularly useful in differentiating platelets and basophils in a blood sample.

Abstract translation: 感兴趣的血细胞通过其背散射特征容易地与血液样品中发现的其它血细胞和样样颗粒区分开。 用于区分血液样本中血小板的优选方法是用辐射束（B'）一次一个地照射细胞和颗粒，并使用多个（B'）检测反向散射（反射）辐射（D'） 的光纤（54）将反向散射辐射（D'）传输到高光电检测器（56），例如， 光电倍增管。 优选地，如此获得的背散射信号与表示例如在规定的相对窄的角度范围内的前向散射（FS）的水平或侧向散射辐射（SS）的水平的第二信号组合， 或由射束中照射的细胞或颗粒的存在或照射的细胞或颗粒的电阻抗引起的细胞照射束的衰减程度，以区分感兴趣的细胞。 本发明的方法和装置特别可用于区分血液样品中的血小板和嗜碱性粒细胞。

公开(公告)号：WO2004017895A2

公开(公告)日：2004-03-04

申请号：PCT/US2003/024426

申请日：2003-08-06

Applicant: COULTER INTERNATIONAL CORP.

Inventor： MAPLES, John A.

IPC: A61K

CPC classification number: A01N1/02 ,  A01N1/0226 ,  G01N33/5002 ,  G01N33/5094 ,  Y10T436/10 ,  Y10T436/108331 ,  Y10T436/173845 ,  Y10T436/200833 ,  Y10T436/202499 ,  Y10T436/2525

Abstract: A stabilization reagent composition, particularly desirable for stabilizing blood samples containing platelets, comprises reactants that generate multiple species of formaldehyde-ammonium complexes; at least one inhibitor of phosphatase enzymatic activity; and at least one inhibitor of protease enzymatic activity. Blood samples and other tissues are stabilized by this composition. Such stabilized samples are produced by methods for stabilizing the tissue by contacting the sample with the composition. Methods for assessing the efficacy of such stabilizing reagents are also included in this invention.

Abstract translation: 稳定化试剂组合物，特别适用于稳定含血小板的血液样品，其包含产生多种甲醛 - 铵复合物的反应物; 至少一种磷酸酶酶活性抑制剂; 和至少一种蛋白酶酶活性抑制剂。 这种组合物可以使血液样本和其他组织稳定。 通过使样品与组合物接触来稳定组织的方法生产这种稳定的样品。 评估这种稳定剂效力的方法也包括在本发明中。

公开(公告)号：WO2003012428A1

公开(公告)日：2003-02-13

申请号：PCT/US2002/022416

申请日：2002-07-15

Applicant: COULTER INTERNATIONAL CORP.

Inventor： HUO, Ziling ,  PARK, Jaesang ,  YAO, Wei ,  ZHANG, Shuliang ,  ZHENG, Min

IPC: G01N31/00

CPC classification number: G01N15/12 ,  G01N15/1456 ,  G01N21/47 ,  G01N33/491 ,  G01N2015/1037 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497

Abstract: A method of analyzing nucleated red blood cells (NRBCs) in a blood sample and further enumerating NRBCs is disclosed. The method includes lysing a first aliquot and a second aliquot of a blood sample separately with a first lysing reagent system and a second lysing reagent system; measuring the first sample mixture in a flow cell by DC, RF, and light scatter measurements; measuring cell distributions and counting remaining blood cells in the second sample mixture by DC impedance measurements in a non-focused flow aperture; analyzing blood cell distribution patterns obtained from measuring the first sample mixture and from measuring the second sample mixture respectively; and further performing a combined analysis to differentiate NRBCs from other cell types and determine numbers of NRBCs in the blood sample.

Abstract translation: 公开了一种分析血液样品中有核红细胞（NRBC）并进一步列举NRBCs的方法。 该方法包括用第一裂解试剂系统和第二裂解试剂系统分别裂解血液样品的第一等分试样和第二等分试样; 通过DC，RF和光散射测量来测量流动池中的第一个样品混合物; 通过在非聚焦流动孔径中的DC阻抗测量来测量细胞分布并计数第二样品混合物中的剩余血细胞; 分析从测量第一样品混合物获得的血细胞分布模式和分别测量第二样品混合物; 并进一步进行组合分析以区分NRBC与其他细胞类型并确定血液样品中NRBC的数目。

公开(公告)号：WO2003003134A1

公开(公告)日：2003-01-09

申请号：PCT/US2002/018415

申请日：2002-06-11

Applicant: COULTER INTERNATIONAL CORP.

Inventor： LI, William, Weigong ,  MINNICH, Helen, A.

IPC: G05D7/00

CPC classification number: G01N35/1097 ,  F16K11/0655 ,  G01F11/16

Abstract: A shear valve assembly comprises a pair of valve pads (10, 20) having opposing planar surfaces (21, 22) supported for relative sliding engagement. Each valve pad defines a plurality of liquid pathways by which a liquid can be passed from one pad to the other. The planar surface of one of the valve pads has an open channel formed therein, such channel having dimensions to accommodate a prescribed volume of liquid. Liquid is introduced into such channel via a first pair of pathways formed in the other valve pad, and liquid is dispensed from the open channel by a second pair of pathways that are brought into alignment with the channel during sliding movement between the valve pads. The valve assembly is particularly useful in blood analyzing instruments for isolating and dispensing relatively minute volumes (of the order of microliters) of whole blood for analysis.

Abstract translation: 剪切阀组件包括一对具有支撑用于相对滑动接合的相对的平坦表面（21,22）的阀垫（10,20）。 每个阀垫限定了多个液体通道，通过该通道液体可以从一个垫传递到另一个垫。 一个阀垫的平坦表面具有形成在其中的开放通道，该通道具有适应规定体积的液体的尺寸。 液体通过形成在另一个阀垫中的第一对通道引入到这种通道中，并且液体通过在阀垫之间的滑动运动期间与通道对准的第二对通道从开放通道分配。 阀组件特别适用于血液分析仪器，用于分离和分配全血的相对微小体积（微升数量级），用于分析。