公开(公告)号：WO2003012428A1

公开(公告)日：2003-02-13

申请号：PCT/US2002/022416

申请日：2002-07-15

Applicant: COULTER INTERNATIONAL CORP.

Inventor： HUO, Ziling ,  PARK, Jaesang ,  YAO, Wei ,  ZHANG, Shuliang ,  ZHENG, Min

IPC: G01N31/00

CPC classification number: G01N15/12 ,  G01N15/1456 ,  G01N21/47 ,  G01N33/491 ,  G01N2015/1037 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497

Abstract: A method of analyzing nucleated red blood cells (NRBCs) in a blood sample and further enumerating NRBCs is disclosed. The method includes lysing a first aliquot and a second aliquot of a blood sample separately with a first lysing reagent system and a second lysing reagent system; measuring the first sample mixture in a flow cell by DC, RF, and light scatter measurements; measuring cell distributions and counting remaining blood cells in the second sample mixture by DC impedance measurements in a non-focused flow aperture; analyzing blood cell distribution patterns obtained from measuring the first sample mixture and from measuring the second sample mixture respectively; and further performing a combined analysis to differentiate NRBCs from other cell types and determine numbers of NRBCs in the blood sample.

Abstract translation: 公开了一种分析血液样品中有核红细胞（NRBC）并进一步列举NRBCs的方法。 该方法包括用第一裂解试剂系统和第二裂解试剂系统分别裂解血液样品的第一等分试样和第二等分试样; 通过DC，RF和光散射测量来测量流动池中的第一个样品混合物; 通过在非聚焦流动孔径中的DC阻抗测量来测量细胞分布并计数第二样品混合物中的剩余血细胞; 分析从测量第一样品混合物获得的血细胞分布模式和分别测量第二样品混合物; 并进一步进行组合分析以区分NRBC与其他细胞类型并确定血液样品中NRBC的数目。