公开(公告)号：WO2022208550A1

公开(公告)日：2022-10-06

申请号：PCT/IN2022/050322

申请日：2022-03-31

Applicant: DR. REDDY'S LABORATORIES LIMITED

Inventor： KANDULA, Rama Bhupal Reddy ,  BANDYOPADHYAY, Suman ,  CHANDRAWANSHI, Vikas ,  VEERAMALLY, Sampath kumar ,  POLISHETTY, Raghavendra ,  BS, Megha

IPC: C07K16/00 ,  C12N5/071

Abstract: The present invention discloses a cell culture method for producing a CTLA-4 fusion protein composition comprising a target % of monomer species of the fusion protein, wherein the cell culture method comprises addition of cysteine in the cell culture medium. The invention further discloses a cell culture process to produce CTLA-4 fusion protein composition with target % of monomer species involving a dual temperature shift with addition of cysteine. Also, the present invention provides a CTLA-4 fusion protein composition comprised of target % of monomer species and/or homodimer species of the CTLA-4 fusion protein.

公开(公告)号：WO2022162700A1

公开(公告)日：2022-08-04

申请号：PCT/IN2022/050078

申请日：2022-01-30

Applicant: DR. REDDY'S LABORATORIES LIMITED

Inventor： KANDULA, Rama Bhupal Reddy ,  BANDYOPADHYAY, Suman ,  CHANDRAWANSHI, Vikas ,  VEERAMALLY, Sampath kumar ,  POLISHETTY, Raghavendra ,  BS, Megha

IPC: C12N5/00 ,  C12P21/00 ,  C07K16/00

Abstract: The present invention discloses a method of cell culture for producing a fusion glycoprotein composition comprising a target glycosylation profile. More particularly, the invention provides a process to produce a glycoprotein composition from mammalian cell culture, wherein the composition comprises a target total sialylated and or di- and tri-sialylated N-glycan variant.