公开(公告)号：WO2019238589A1

公开(公告)日：2019-12-19

申请号：PCT/EP2019/065044

申请日：2019-06-07

Applicant: DUBLIN CITY UNIVERSITY

Inventor： KEYES, Tia ,  SAKALYS, Rokas ,  KHO, Kiang, Wei ,  STEPLEWSKA, Agata ,  GIMENEZ, Aurelien ,  SARANGI, Nirod, Kumar

IPC: G01N33/543 ,  B01L3/00 ,  B01L9/00 ,  G01N27/02 ,  G01N33/487

Abstract: A method of assaying the interaction of a molecule and a lipid bilayer is described. The method employs a microfluidic device comprising a substrate having at least one concave microcavity with a metallic surface defining an aperture and a liquid disposed within the microcavity, a lipid bilayer suspended across the aperture, and a microfluidic channel containing a liquid disposed on top of the substrate in fluid communication with the lipid bilayer. The method comprises the steps of passing a liquid containing a test molecule across the microfluidic channel, and monitoring lipid bilayer molecule interactions by plasmonically enhanced Raman or fluorescence spectroscopy configured for plasmonic enhancement of a detection signal evolving from the test molecule or lipid bilayer. Also described in a microfluidic device configured to perform the method of the invention.