公开(公告)号：WO1998028325A1

公开(公告)日：1998-07-02

申请号：PCT/US1997023428

申请日：1997-12-19

Applicant: EMORY UNIVERSITY

Inventor： EMORY UNIVERSITY ,  HAGEDORN, Curt, H. ,  SPIVAK-KROIZMAN, Taly ,  XIE, Yiping

IPC: C07K01/22

CPC classification number: C07K14/4705

Abstract: The invention provides a method of purifying human eIF-4E protein and amino acid sequence variants thereof having altered binding affinity for capped RNA. Using the described purification, amino acid sequence variants can readily be expressed, purified and tested. Both lowered and enhanced binding affinity variants are useful for modifying protein expression levels in vivo and in vitro.

Abstract translation: 本发明提供了一种纯化人eIF-4E蛋白质的方法，其具有对加帽RNA具有改变的结合亲和力的氨基酸序列变体。 使用所述纯化，氨基酸序列变体可以容易地进行表达，纯化和测试。 降低和增强的结合亲和力变体都可用于体内和体外修饰蛋白质表达水平。