公开(公告)号：WO1997026602A1

公开(公告)日：1997-07-24

申请号：PCT/US1997000520

申请日：1997-01-14

Applicant: ENVIRONMENTAL RESEARCH INSTITUTE OF MICHIGAN

Inventor： ENVIRONMENTAL RESEARCH INSTITUTE OF MICHIGAN ,  VOGT, Robert, C.

IPC: G06F15/00

CPC classification number: G06K9/00127

Abstract: The extraction and pre-cueing of axon fibers and clusters is performed on transmission electron micrograph (TEM) image mosaics to reduce neuroanatomist workload. Given a multiplicity of myeling-sheathed axon fibers in a cross-sectional image containing many such fibers, the inventive method uses these grey-level operators to effectively identify co-occurrences of the axon fibers and their myelin sheaths and output a result representative of these co-occurences. The co-occurrences may then be sorted in terms of their geometry to identify groups of fibers indicative of axon clusters. Specifically, a grey-level opening and conditional dilation is performed on the image to obtain a first residue representative of potential axons. A grey-level closing and conditional erosion of the first opening image is then performed to obtain a second residue representative of potential axon fibers, including their respective myelin sheaths. The first residue may then be used as a marker to conditionally dilate over the second residue to identify the co-occurrences. The method of the invention significantly reduces the workload of the reviewers by identifying rougly 95 percent of the axons, with only a one to two percent false alarm rate, based on a reasonable computation time of about one hour per sample on a fast workstation.

Abstract translation: 在透射电子显微镜（TEM）图像马赛克中进行轴突纤维和簇的提取和预提示，以减少神经解剖学家的工作量。 给定在包含许多这样的纤维的横截面图像中的多个髓鞘护套的轴突纤维，本发明的方法使用这些灰度级操作符来有效地识别轴突纤维及其髓鞘的共同出现，并输出代表这些纤维的结果 共同出现次数。 然后可以根据它们的几何结构对共同出现进行分类，以鉴定指示轴突簇的纤维组。 具体地，对图像进行灰度开放和条件扩张以获得表示潜在轴突的第一残基。 然后执行第一开放图像的灰度闭合和有条件的侵蚀，以获得代表潜在轴突纤维的第二残基，包括它们各自的髓鞘。 然后可以将第一残余物用作标记物以在第二残基上有条件地扩张以鉴定共同发生。 基于在快速工作站上每个样本的合理计算时间约1小时，本发明的方法通过以95％的轴突识别出只有一到两个百分点的误报率，从而显着降低了审稿人的工作量。