公开(公告)号：WO2018069756A1

公开(公告)日：2018-04-19

申请号：PCT/IB2017/000465

申请日：2017-04-04

Applicant: FERTILESAFE LTD.

Inventor： ARAV, Amir ,  NATAN, Yehudit ,  PATRIZIO, Pasquale

IPC: A01N1/02

CPC classification number: A01N1/0242 ,  A01N1/0257 ,  A01N1/0268 ,  A01N1/0284 ,  B01L7/02 ,  B01L7/5255

Abstract: A system (1000) for use in warming, thawing, cooling, and reconstituting a cryopreserved biological sample (102) is disclosed, which includes a heating system (1100) including a container with an insulating wall defining a chamber to retain a cryogenic fluid (1104), and a receiving space to removably receive a receptacle configured and dimensioned to retain a warming solution (1106). The sample is movable from the cryogenic fluid into the warming solution in an interval of time that results in reduced air exposure and an increased warming rate. A cooling system (1200) is also disclosed for cooling the warmed sample to e.g. room temperature, which includes a repository (1202) that accommodates a series of containers (1204A-1204D) including respective solutions, which achieves simultaneous purging of cryoprotectant from the biological sample, and cooling of the biological sample. A retaining device comprising a tubular member (straw) and a perforated member is also disclosed.

Abstract translation: 公开了一种用于加温，融化，冷却和重构冷冻保存的生物样品（102）的系统（1000），其包括加热系统（1100），该加热系统包括具有绝缘壁的容器 用于保持低温流体（1104）的腔室，以及用于可拆卸地接收配置和定尺寸为保持加温溶液（1106）的容器的接收空间。 样品可以在一段时间内从低温流体移动到加温溶液中，导致空气暴露减少和升温速率增加。 还公开了一种冷却系统（1200），用于将加热的样品冷却至例如 室温，其包括容纳包含各自溶液的一系列容器（1204A-1204D）的储存库（1202），其实现从生物样品同时清除冷冻保护剂并冷却生物样品。 还公开了包括管状构件（吸管）和穿孔构件的保持装置。