公开(公告)号：WO2019142047A3

公开(公告)日：2019-07-25

申请号：PCT/IB2019/000037

申请日：2019-01-11

Applicant: FERTILESAFE LTD.

Inventor： ARAV, Amir

IPC: A01N1/02

Abstract: Methods and devices for freeze-drying a biological sample of mammalian cells or tissue are disclosed. A method may include placing a biological sample on or in a structure such as a pre-cooled metal surface to decrease a temperature of the biological sample and with the biological sample in a closed chamber applying a vacuum to the chamber to lower a pressure within the chamber, cooling the chamber to lower a temperature within the chamber and applying heat to the biological sample within the chamber. The biological sample can include one or more of stem cells, hematopoietic stem cells, mesenchymal stem cells, embryonic stem cells, induced pluripotent stem cells, sperm, oocytes, embryos, ovarian tissue, uterine tissue or testicular tissue. A method for rehydrating samples is also disclosed.

公开(公告)号：WO2016001933A1

公开(公告)日：2016-01-07

申请号：PCT/IL2015/050691

申请日：2015-07-03

Applicant: FERTILESAFE LTD

Inventor： ARAV, Amir

IPC: A01N1/02

CPC classification number: A01N1/0268 ,  A01N1/0236

Abstract: The present invention discloses a device for preparing a biological sample for vitrification, and a method for utilization thereof. The device comprises a chamber, an inlet, and a first drain. The chamber is configured to contain a first liquid and to accommodate a carrier for holding the sample immersed in the first liquid. The inlet is configured to add a second liquid into the chamber for modifying a composition of the first liquid. The first drain is configured to drain the first liquid from the chamber. The chamber has a liquid capacity that is sufficiently large to enable a gradual modification of the composition in accordance with a modification course adequate for preparing the sample for vitrification.

Abstract translation: 本发明公开了一种用于制备用于玻璃化的生物样品的装置及其使用方法。 该装置包括腔室，入口和第一排水管。 该室被构造成容纳第一液体并容纳用于保持浸没在第一液体中的样品的载体。 入口构造成将第二液体添加到室中用于改变第一液体的组成。 第一排水口构造成从腔室排出第一液体。 该室具有足够大的液体容量，以使根据适于制备用于玻璃化的样品的修饰过程逐渐改变组合物。

公开(公告)号：WO2018069756A1

公开(公告)日：2018-04-19

申请号：PCT/IB2017/000465

申请日：2017-04-04

Applicant: FERTILESAFE LTD.

Inventor： ARAV, Amir ,  NATAN, Yehudit ,  PATRIZIO, Pasquale

IPC: A01N1/02

CPC classification number: A01N1/0242 ,  A01N1/0257 ,  A01N1/0268 ,  A01N1/0284 ,  B01L7/02 ,  B01L7/5255

Abstract: A system (1000) for use in warming, thawing, cooling, and reconstituting a cryopreserved biological sample (102) is disclosed, which includes a heating system (1100) including a container with an insulating wall defining a chamber to retain a cryogenic fluid (1104), and a receiving space to removably receive a receptacle configured and dimensioned to retain a warming solution (1106). The sample is movable from the cryogenic fluid into the warming solution in an interval of time that results in reduced air exposure and an increased warming rate. A cooling system (1200) is also disclosed for cooling the warmed sample to e.g. room temperature, which includes a repository (1202) that accommodates a series of containers (1204A-1204D) including respective solutions, which achieves simultaneous purging of cryoprotectant from the biological sample, and cooling of the biological sample. A retaining device comprising a tubular member (straw) and a perforated member is also disclosed.

Abstract translation: 公开了一种用于加温，融化，冷却和重构冷冻保存的生物样品（102）的系统（1000），其包括加热系统（1100），该加热系统包括具有绝缘壁的容器 用于保持低温流体（1104）的腔室，以及用于可拆卸地接收配置和定尺寸为保持加温溶液（1106）的容器的接收空间。 样品可以在一段时间内从低温流体移动到加温溶液中，导致空气暴露减少和升温速率增加。 还公开了一种冷却系统（1200），用于将加热的样品冷却至例如 室温，其包括容纳包含各自溶液的一系列容器（1204A-1204D）的储存库（1202），其实现从生物样品同时清除冷冻保护剂并冷却生物样品。 还公开了包括管状构件（吸管）和穿孔构件的保持装置。

公开(公告)号：WO2016005971A1

公开(公告)日：2016-01-14

申请号：PCT/IL2015/050698

申请日：2015-07-06

Applicant: FERTILESAFE LTD

Inventor： ARAV, Amir

IPC: C12M3/00

CPC classification number: C12M29/10 ,  C12M21/08 ,  C12M29/18 ,  C12M41/16

Abstract: The present invention discloses a device for culturing a biological sample in a culturing fluid. The device comprises a culturing module. The culturing module is configured to hold a first portion of the culturing fluid in continuous flow and to allow culturing the biological sample in the first portion, wherein the culturing fluid in the first portion is substantially in a physiological temperature of the biological sample. The device may also comprise a chamber, configured to hold a second portion of the culturing fluid in a hollow space of the chamber wherein the culturing fluid in the second portion is colder than the temperature of the fluid in the first portion.

Abstract translation: 本发明公开了一种在培养液中培养生物样品的装置。 该装置包括培养模块。 培养模块被配置为将培养液的第一部分保持在连续流中并允许在第一部分中培养生物样品，其中第一部分中的培养液基本上处于生物样品的生理温度。 该装置还可以包括腔室，其构造成将培养流体的第二部分保持在腔室的中空空间中，其中第二部分中的培养流体比第一部分中的流体的温度更冷。

公开(公告)号：WO2019142047A2

公开(公告)日：2019-07-25

申请号：PCT/IB2019/000037

申请日：2019-01-11

Applicant: FERTILESAFE LTD.

Inventor： ARAV, Amir

IPC: A01N1/02

Abstract: A method for freeze-drying a biological sample of mammalian cells or tissue including placing a biological sample on or in a structure to increase a temperature of the biological sample and with the biological sample in a closed chamber applying a vacuum to the chamber to lower a pressure within the chamber, cooling the chamber to lower a temperature within the chamber and applying heat to the biological sample within the chamber. The biological sample can include one or more of stem cells, hematopoietic stem cells, mesenchymal stem cells, embryonic stem cells, induced pluripotent stem cells, sperm, oocytes, embryos, ovarian tissue, uterine tissue or testicular tissue.

公开(公告)号：WO2017122210A1

公开(公告)日：2017-07-20

申请号：PCT/IL2017/050044

申请日：2017-01-13

Applicant: FERTILESAFE LTD.

Inventor： ARAV, Amir

IPC: A01N1/02

CPC classification number: A01N1/0242 ,  A01N1/0268

Abstract: The present Invention discloses automatic devices configured to perform a cryoprocedure on at least one biological sample carried by one or more carriers. The device comprising a carrier holder, a container holder, a carrier driver and a container driver. The carrier holder is configured to receive and hold the one or more carriers in an upright orientation while holding the at least one biological sample. The container holder is configured to hold two or more containers each in a predetermined location on the container holder. The carrier driver is configured to translate the carrier holder. And the container driver is configured to translate the predetermined locations so as to position one of them in a position accessible to the carrier holder, so as to enable the carrier driver to submerge an active portion of each one or more carriers held by the carrier holder in a predetermined vertical depth in the position accessible to the carrier holder.

Abstract translation: 本发明公开了配置为对由一个或多个载体携带的至少一个生物样本执行低温过程的自动装置。 该装置包括载体保持器，容器保持器，载体驱动器和容器驱动器。 载体保持器构造成在保持至少一个生物样本的同时以竖直定向接收并保持一个或多个载体。 容器保持器构造成将两个或更多个容器各自保持在容器保持器上的预定位置。 载体驱动器被配置为平移载体保持器。 并且容器驱动器被构造成平移预定位置以将它们中的一个定位在载体保持器可接近的位置中，以使得载体驱动器能够浸没由载体保持器保持的每个一个或多个载体的活动部分 在载体支架可接近的位置预定的垂直深度中。

公开(公告)号：WO2017064715A1

公开(公告)日：2017-04-20

申请号：PCT/IL2016/051115

申请日：2016-10-13

Applicant: FERTILESAFE LTD.

Inventor： ARAV, Amir

IPC: A01N1/02 ,  C12N1/04 ,  C12M3/00

CPC classification number: A01N1/0268

Abstract: The present Invention discloses devices configured to perform a cryoprocedure on at least one biological sample and methods for using the devices. The disclosed devices comprise a straw and a pod. The straw comprises a straw space, configured to draw liquid from a distal end of the straw space towards a proximal end of the straw space. The pod is coupled to a distal end of the straw and comprises a perforated member comprises at least one orifice whose diameter is smaller than the diameter of the at least one biological sample, wherein the perforated element is configured to allow inflow of liquids into the preparation space and outflow of liquids from the preparation space. The holding space is configured to form, together with the straw space, a preparation space wherein the at least one biological sample can undergo the cryoprocedure.

Abstract translation: 本发明公开了被配置为对至少一种生物样品进行低温处理的装置以及使用该装置的方法。 所公开的装置包括吸管和吊舱。 该吸管包括吸管空间，该吸管空间构造成从吸管空间的远端朝向吸管空间的近端吸取液体。 所述荚与所述吸管的远端连接并且包括穿孔构件，所述穿孔构件包括直径小于所述至少一个生物样本的直径的至少一个孔，其中所述穿孔元件构造成允许液体流入所述制剂 空间和液体从准备空间流出。 容纳空间构造成与吸管空间一起形成准备空间，其中至少一个生物样本可以经历低温过程。

公开(公告)号：WO2016016886A1

公开(公告)日：2016-02-04

申请号：PCT/IL2015/050771

申请日：2015-07-27

Applicant: FERTILESAFE LTD

Inventor： ARAV, Amir

IPC: A01N1/02 ,  B65D53/02 ,  B65D81/18 ,  B65D51/16 ,  B65D41/32

CPC classification number: A01N1/0268 ,  A01N1/0257

Abstract: Devices and methods for cryopreserving a biological sample by liquid nitrogen, the device comprising: a first container comprising an internal space, the first container is configured to be submerged in liquid nitrogen stored in a cryopreservation space inside a liquid nitrogen container and to hold the biological sample in the internal space while preventing contact of the biological sample with the liquid nitrogen; and an airway comprising a first end and a second end, the airway is coupled to the first container at its first end and configured to couple the internal space to an outer environment, external to the liquid nitrogen container, so as to balance pressure changes inside the internal space.

Abstract translation: 用于通过液氮冷冻生物样品的装置和方法，所述装置包括：包含内部空间的第一容器，所述第一容器被配置为浸没在储存在液氮容器内的冷冻保存空间内的液氮中并保持所述生物样品 在内部空间中取样，同时防止生物样品与液氮接触; 以及包括第一端和第二端的气道，所述气道在其第一端处联接到所述第一容器并且被配置为将所述内部空间耦合到所述液氮容器外部的外部环境，以平衡所述液氮容器内部的压力变化 内部空间。

公开(公告)号：WO2014178058A1

公开(公告)日：2014-11-06

申请号：PCT/IL2014/050399

申请日：2014-05-01

Applicant: FERTILESAFE LTD

Inventor： ARAV, Amir

IPC: B01D53/00 ,  B01D5/00 ,  F25D3/10

CPC classification number: F25J1/0012 ,  B01D5/0003 ,  B01D53/002 ,  B01D53/261 ,  B01D2253/102 ,  B01D2253/20 ,  B01D2257/80 ,  B01D2257/91 ,  B01D2258/06 ,  B01D2259/804 ,  F25J1/0221 ,  F25J1/0251 ,  F25J1/0276 ,  F25J2210/42

Abstract: The present invention discloses methods and devices for continuously producing liquid air. The present invention comprises bringing a first container confining an unpressurised first volume coupled to outer air in contact with a first cryogenic medium, and maintaining a temperature of the first cryogenic medium substantially higher than the boiling temperature of the first cryogenic medium for affecting continues production of liquid air.

Abstract translation: 本发明公开了连续生产液体空气的方法和装置。 本发明包括使第一容器限制与第一低温介质接触的外部空气耦合的未加压的第一容积，并且保持第一低温介质的温度显着高于第一低温介质的沸腾温度，以影响继续生产 液体空气。