公开(公告)号：WO2016075207A1

公开(公告)日：2016-05-19

申请号：PCT/EP2015/076357

申请日：2015-11-11

Applicant: GE HEALTHCARE BIO-SCIENCES AB

Inventor： NYHOLM, Lena ,  ARTHURSSON, Johan ,  OLSSON, Eva, Cecilia ,  OHMAN, Ulrika

IPC: G01N30/86 ,  G01N30/26 ,  G01N30/50

CPC classification number: G01N30/8658 ,  G01N30/26 ,  G01N30/50 ,  G01N30/86

Abstract: Method for determining the influence of least a first and a second experimental parameter on a liquid chromatography protocol for purifying one or more target molecules from a sample, comprising the steps: - performing chromatography purifications of the sample at a plurality of different experimental conditions where at least the first and the second experimental parameter each are varied over a predetermined range, each purification being registered as a chromatogram by monitoring an output parameter indicative of the purification result during the purification; and - displaying in a graphical user interface at least a subset of the registered chromatograms as chromatogram-miniatures in an evaluation diagram wherein the position of each displayed chromatogram-miniature is determined by the experimental parameters for the corresponding purification, thereby allowing a user to visually determine trends and the influence of the experimental parameters on the liquid chromatography protocol.

Abstract translation: 用于确定用于从样品中纯化一种或多种靶分子的液体色谱方案的至少第一和第二实验参数的影响的方法，包括以下步骤： - 在多个不同实验条件下进行所述样品的色谱纯化，其中在 至少第一和第二实验参数各自在预定范围内变化，通过监测在净化过程中指示纯化结果的输出参数，将每个纯化作为色谱图注册; 并且在图形用户界面中将所注册的色谱图的至少一个子集显示为评估图中的色谱图缩略图，其中每个显示的色谱图微型的位置由相应纯化的实验参数确定，从而允许用户视觉上 确定趋势和实验参数对液相色谱方案的影响。

公开(公告)号：WO2017174742A1

公开(公告)日：2017-10-12

申请号：PCT/EP2017/058287

申请日：2017-04-06

Applicant: GE HEALTHCARE BIO-SCIENCES AB

Inventor： BANGTSSON, Petra ,  NYHOLM, Lena ,  GORANSSON, Bengt

IPC: G01N30/82 ,  G01N30/86

CPC classification number: G01N30/82 ,  B01D15/247 ,  G01N30/8658 ,  G01N30/8662 ,  G01N30/8696

Abstract: The present invention relates to a method for providing information from a fraction collector in a liquid chromatography system, the system comprising a column, at least one reservoir for containing a fluid, at least one pump connected to said reservoir, a column arranged to receive an input from the reservoir, a measuring device for performing measurements on a fluid output from the column, and a fraction collector for receiving said fluid output in a plurality of receiving vessels, wherein the method comprises - determining at least one parameter of the fraction collector, and - providing information of said parameter on a display in the form of a graphical representation.

Abstract translation: 本发明涉及一种用于从液相色谱系统中的馏分收集器提供信息的方法，所述系统包括塔，至少一个用于容纳流体的储存器，至少一个泵，所述泵连接到所述 储存器，布置成接收来自储存器的输入的塔，用于对从柱输出的流体进行测量的测量装置，以及用于在多个接收容器中接收所述流体输出的馏分收集器，其中该方法包括： - 在 分数收集器的至少一个参数，以及 - 以图形表示的形式在显示器上提供所述参数的信息。

公开(公告)号：WO2016079061A1

公开(公告)日：2016-05-26

申请号：PCT/EP2015/076704

申请日：2015-11-16

Applicant: GE HEALTHCARE BIO-SCIENCES AB

Inventor： NYHOLM, Lena ,  HEDKVIST, Lotta ,  ARTHURSSON, Johan ,  OLSSON, Eva, Cecilia ,  LARSSON, Henrik

IPC: G01N30/82 ,  B01D15/24 ,  G01N30/86

CPC classification number: G01N30/8631 ,  B01D15/247 ,  G01N30/82 ,  G01N30/8644

Abstract: The present invention relates to a method for performing liquid chromatography purification of one or more target molecules from a sample comprising: providing an eluent flow having one or more target molecules, measuring an output parameter indicative of the content of the one or more target molecules in the eluent flow, storing output parameter data, and dividing the eluent flow into consecutive eluent fractions, dividing the output parameter data into corresponding data fractions, in each data fraction obtaining a value indicative of characteristic behavior of the measured output parameter,identifying trends in the measured output parameter based upon the obtained value in consecutive data fractions, and identifying peak(s)in the measured output parameter correlated to eluent fractions based upon the identified trends, whereby information of identified peak(s) and correlated eluent fraction(s) can be presented and purified sample(s) from the eluent may be collected.

Abstract translation: 本发明涉及一种用于从样品中进行液相色谱纯化一种或多种靶分子的方法，包括：提供具有一种或多种靶分子的洗脱液流，测量指示一种或多种靶分子含量的输出参数 洗脱液流动，存储输出参数数据，并将洗脱液流分为连续的洗脱液分数，将输出参数数据除以相应的数据分数，在每个数据馏分中获得表示测量输出参数的特征行为的值， 基于连续数据分数中获得的值测量的输出参数，以及基于所识别的趋势，确定与洗脱液分数相关的测量输出参数中的峰，由此鉴定的峰和相关的洗脱分数的信息可以 可以收集来自洗脱液的纯化样品。