公开(公告)号：WO2016075207A1

公开(公告)日：2016-05-19

申请号：PCT/EP2015/076357

申请日：2015-11-11

Applicant: GE HEALTHCARE BIO-SCIENCES AB

Inventor： NYHOLM, Lena ,  ARTHURSSON, Johan ,  OLSSON, Eva, Cecilia ,  OHMAN, Ulrika

IPC: G01N30/86 ,  G01N30/26 ,  G01N30/50

CPC classification number: G01N30/8658 ,  G01N30/26 ,  G01N30/50 ,  G01N30/86

Abstract: Method for determining the influence of least a first and a second experimental parameter on a liquid chromatography protocol for purifying one or more target molecules from a sample, comprising the steps: - performing chromatography purifications of the sample at a plurality of different experimental conditions where at least the first and the second experimental parameter each are varied over a predetermined range, each purification being registered as a chromatogram by monitoring an output parameter indicative of the purification result during the purification; and - displaying in a graphical user interface at least a subset of the registered chromatograms as chromatogram-miniatures in an evaluation diagram wherein the position of each displayed chromatogram-miniature is determined by the experimental parameters for the corresponding purification, thereby allowing a user to visually determine trends and the influence of the experimental parameters on the liquid chromatography protocol.

Abstract translation: 用于确定用于从样品中纯化一种或多种靶分子的液体色谱方案的至少第一和第二实验参数的影响的方法，包括以下步骤： - 在多个不同实验条件下进行所述样品的色谱纯化，其中在 至少第一和第二实验参数各自在预定范围内变化，通过监测在净化过程中指示纯化结果的输出参数，将每个纯化作为色谱图注册; 并且在图形用户界面中将所注册的色谱图的至少一个子集显示为评估图中的色谱图缩略图，其中每个显示的色谱图微型的位置由相应纯化的实验参数确定，从而允许用户视觉上 确定趋势和实验参数对液相色谱方案的影响。

公开(公告)号：WO2019228858A1

公开(公告)日：2019-12-05

申请号：PCT/EP2019/063075

申请日：2019-05-21

Applicant: GE HEALTHCARE BIO-SCIENCES AB

Inventor： BANGTSSON, Petra ,  ARTHURSSON, Johan ,  HYCKENBERG, Key ,  HEDKVIST, Lotta

IPC: G06T7/00

Abstract: The present invention relates to a method of automated configuration in a bioprocess system (10)and to verify a process defined by a flow path representation. The method comprises the steps of: -capturing one image of the bioprocess system (10) comprising tubing (50) for the fluid communication between units of the bioprocess system (10); -analyzing the captured image to identify the tubing (50) connecting units of the bioprocess system (10); -producing a processed representation from the captured image wherein at least a part of the tubing (50) is identified;and -comparing the processed representation with the flow path representation to verify their functional concordance.

公开(公告)号：WO2016079061A1

公开(公告)日：2016-05-26

申请号：PCT/EP2015/076704

申请日：2015-11-16

Applicant: GE HEALTHCARE BIO-SCIENCES AB

Inventor： NYHOLM, Lena ,  HEDKVIST, Lotta ,  ARTHURSSON, Johan ,  OLSSON, Eva, Cecilia ,  LARSSON, Henrik

IPC: G01N30/82 ,  B01D15/24 ,  G01N30/86

CPC classification number: G01N30/8631 ,  B01D15/247 ,  G01N30/82 ,  G01N30/8644

Abstract: The present invention relates to a method for performing liquid chromatography purification of one or more target molecules from a sample comprising: providing an eluent flow having one or more target molecules, measuring an output parameter indicative of the content of the one or more target molecules in the eluent flow, storing output parameter data, and dividing the eluent flow into consecutive eluent fractions, dividing the output parameter data into corresponding data fractions, in each data fraction obtaining a value indicative of characteristic behavior of the measured output parameter,identifying trends in the measured output parameter based upon the obtained value in consecutive data fractions, and identifying peak(s)in the measured output parameter correlated to eluent fractions based upon the identified trends, whereby information of identified peak(s) and correlated eluent fraction(s) can be presented and purified sample(s) from the eluent may be collected.

Abstract translation: 本发明涉及一种用于从样品中进行液相色谱纯化一种或多种靶分子的方法，包括：提供具有一种或多种靶分子的洗脱液流，测量指示一种或多种靶分子含量的输出参数 洗脱液流动，存储输出参数数据，并将洗脱液流分为连续的洗脱液分数，将输出参数数据除以相应的数据分数，在每个数据馏分中获得表示测量输出参数的特征行为的值， 基于连续数据分数中获得的值测量的输出参数，以及基于所识别的趋势，确定与洗脱液分数相关的测量输出参数中的峰，由此鉴定的峰和相关的洗脱分数的信息可以 可以收集来自洗脱液的纯化样品。