公开(公告)号：WO2013040552A3

公开(公告)日：2013-05-10

申请号：PCT/US2012055750

申请日：2012-09-17

申请人： GEORGIA HEALTH SCIENCES UNIVERSITY

发明人： MELLOR ANDREW L ,  MUNN DAVID H ,  HUANG LEI ,  SHARMA MADHAV

IPC分类号： C12N15/117 ,  A61K31/713 ,  C12N15/11

CPC分类号： A61K47/34 ,  A61K39/02 ,  A61K2039/52 ,  A61K2039/55511 ,  C12N15/111 ,  C12N15/117 ,  C12N2310/17 ,  C12N2320/53

摘要： Compositions including a polynucleotide combined with a vehicle and methods of their use to induce a suppressive immune response are provided. In some embodiments the compositions induce an increase in expression of indoleamine 2,3 dioxygenase (IDO) enzyme activity in cells. The methods and compositions can be used to inhibit or reduce immune- mediated tissue destruction, to treat autoimmune diseases and inflammatory responses, to promote immune tolerance, to enhance tolerizing vaccines, to treat allergies, to treat asthma, or to enhance mucosal tolerance in subject. Methods and compositions for inducing a suppressive immune response for while minimizing undesirable side effects in the subject are also provided. An exemplary undesirable side effect is systemic release of INFgamma. Exemplary compositions that can be used to induce an immune response in a subject without inducing systemic release of INFgamma include compositions containing a polynucleotide lacking an immunostimulatory nucleic acid sequence complexed with a carrier.

摘要翻译： 提供了包含与载体组合的多核苷酸的组合物及其用于诱导抑制性免疫应答的方法。 在一些实施方案中，组合物诱导细胞中吲哚胺2,3双加氧酶（IDO）酶活性表达的增加。 所述方法和组合物可用于抑制或减少免疫介导的组织破坏，治疗自身免疫疾病和炎症反应，促进免疫耐受，增强耐受疫苗，治疗变态反应，治疗哮喘或增强对象的粘膜耐受性 。 还提供了用于诱导抑制性免疫应答同时使受试者中不希望的副作用最小化的方法和组合物。 示例性的不良副作用是INFγ的全身释放。 可用于在受试者中诱导免疫应答而不诱导INFγ全身释放的示例性组合物包括含有缺乏与载体复合的免疫刺激性核酸序列的多核苷酸的组合物。