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    STABLE CELL LINES FOR RETROVIRAL PRODUCTION
    2.
    发明申请
    STABLE CELL LINES FOR RETROVIRAL PRODUCTION 审中-公开
    稳定的细胞系用于逆转录病毒生产

    公开(公告)号：WO2017089308A1

    公开(公告)日：2017-06-01

    申请号：PCT/EP2016/078336

    申请日：2016-11-21

    申请人： GLAXOSMITHKLINE INTELLECTUAL PROPERTY DEVELOPMENT LIMITED

    发明人： JOHNSON, Sabine ,  PALLANT, Celeste ,  VAMVA, Eirini ,  VINK, Conrad

    IPC分类号： C12N5/10 ,  C12N7/02 ,  C12N7/04 ,  C12N15/85

    CPC分类号： C12N7/00 ,  C12N5/10 ,  C12N7/025 ,  C12N7/045 ,  C12N15/85 ,  C12N15/86 ,  C12N2740/15043 ,  C12N2740/16043 ,  C12N2740/16052 ,  C12N2830/006 ,  C12N2830/40 ,  C12N2830/60

    摘要： The invention relates to retroviral producer cell comprising nucleic acid sequences encoding: gag and pol proteins; envelope protein or a functional substitute thereof; and the RNA genome of the retroviral vector particle, wherein said nucleic acid sequences are all located at a single locus within the retroviral producer cell genome. The invention also relates to nucleic acid vectors comprising a non-mammalian origin of replication and the ability to hold at least 25 kilobases (kb) of DNA, characterized in that said nucleic acid vector comprises retroviral nucleic acid sequences encoding: gag and pol proteins, and an env protein or a functional substitute thereof. The invention also relates to uses and methods using said nucleic acid vector in order to produce stable retroviral packaging and producer cell lines.

    摘要翻译： 本发明涉及逆转录病毒生产细胞，其包含编码gag和pol蛋白的核酸序列; 包膜蛋白或其功能性替代物; 和逆转录病毒载体颗粒的RNA基因组，其中所述核酸序列全部位于逆转录病毒生产细胞基因组内的单个基因座。 本发明还涉及包含非哺乳动物复制起点和保持至少25千碱基（kb）DNA的能力的核酸载体，其特征在于所述核酸载体包含编码gag和pol蛋白的逆转录病毒核酸序列， 和env蛋白或其功能性替代物。 本发明还涉及使用所述核酸载体以产生稳定的逆转录病毒包装和生产者细胞系的用途和方法。