公开(公告)号：WO2005089040A2

公开(公告)日：2005-09-29

申请号：PCT/DK2005000205

申请日：2005-03-23

Applicant: HANSENS LAB ,  HARBOE MARIANNE KIRSTEN ,  MAARTEN VAN DEN BRINK JOHANNES ,  JOHANSEN ERIC ,  RAHBEK-NIELSEN HENRIK ,  LARSEN RAUL ,  RAMPILLI MARCO

Inventor： HARBOE MARIANNE KIRSTEN ,  MAARTEN VAN DEN BRINK JOHANNES ,  JOHANSEN ERIC ,  RAHBEK-NIELSEN HENRIK ,  LARSEN RAUL ,  RAMPILLI MARCO

IPC: C12N9/64

CPC classification number: C12N9/6483 ,  C12Y304/23004

Abstract: The invention relates to a preparative method of obtaining a highly purified preparation of an aspartic protease, chymosin C. The invention further relates to the use of chymosin C in milk coagulating compositions in cheese manufacturing based on cow's milk and milk from any animal species, which are used in cheese.

Abstract translation: 本发明涉及一种获得天冬氨酸蛋白酶，凝乳酶C的高度纯化制剂的制备方法。本发明还涉及基于来自任何动物物种的牛奶和奶的奶酪制造中乳凝素组合物中乳糖凝胶C的用途，其中 用于奶酪。

公开(公告)号：WO9529999A3

公开(公告)日：1996-02-08

申请号：PCT/DK9500171

申请日：1995-04-25

Applicant: HANSENS LAB ,  LARSEN ROBERT ,  HARBOE MARIANNE KIRSTEN ,  LADSTEN PAAL

Inventor： LARSEN ROBERT ,  HARBOE MARIANNE KIRSTEN ,  LADSTEN PAAL

IPC: C12N9/64 ,  C12N9/96

CPC classification number: C12N9/6481 ,  C12N9/96

Abstract: A process of separating milk clotting enzymes in extracts of animal stomach tissue which comprises contacting a partially purified extract with an ion exchange resin under conditions where chymosin is bound to the resin and recovering the chymosin, the process optionally comprising the further step of recovering pepsin, from a pepsin-rich extract fraction using an ion exchange resin, and a liquid or powdered rennet composition containing at least 90 % of its milk clotting activity as chymosin activity, the composition comprising a chymosin stabilizing agent which is typically selected from a protein, a peptide, an amino acid or ascorbic acid.

公开(公告)号：WO02064759A8

公开(公告)日：2004-04-29

申请号：PCT/DK0200083

申请日：2002-02-06

Applicant: HANSENS LAB ,  HARBOE MARIANNE KIRSTEN

Inventor： HARBOE MARIANNE KIRSTEN

IPC: C12N9/64 ,  C12N9/99

CPC classification number: C12N9/6478 ,  C12N9/99

Abstract: A method of providing polypeptide preparations having a reduced content of undesired enzymatic side activities, the method comprising subjecting a medium containing a desired polypeptide such as an enzyme, a pharmaceutically active or immunologically active polypeptide to a pH of less than 2 for a period of time that is required to inactivate the side activities whilst retaining the activity of the desired polypeptide. The method is useful for providing milk clotting enzyme products including rennets or coagulants based on chymosin or pepsin or microbial aspartic proteases e.g. derived from bacterial species and species of filamentous fungi.

Abstract translation: 一种提供多肽制剂的方法，所述多肽制剂具有减少的不期望的酶促侧活性的含量，所述方法包括使含有所需多肽（例如酶，药学活性或免疫活性多肽）的培养基在小于2的pH下经历一段时间 需要在保留所需多肽的活性的同时灭活侧活性。 该方法可用于提供乳凝固酶产品，包括基于凝乳酶或胃蛋白酶或微生物天冬氨酸蛋白酶的乳杆菌或凝血剂。 衍生自细菌种类和丝状真菌的种类。

公开(公告)号：WO02064759A2

公开(公告)日：2002-08-22

申请号：PCT/DK0200083

申请日：2002-02-06

Applicant: HANSENS LAB ,  HARBOE MARIANNE KIRSTEN

Inventor： HARBOE MARIANNE KIRSTEN

IPC: C12N9/64 ,  C12N9/99 ,  C12N9/00

CPC classification number: C12N9/6478 ,  C12N9/99

Abstract: A method of providing polypeptide preparations having a reduced content of undesired enzymatic side activities, the method comprising subjecting a medium containing a desired polypeptide such as an enzyme, a pharmaceutically active or immunologically active polypeptide to a pH of less than 2 for a period of time that is required to inactivate the side activities whilst retaining the activity of the desired polypeptide. The method is useful for providing milk clotting enzyme products including rennets or coagulants based on chymosin or pepsin or microbial aspartic proteases e.g. derived from bacterial species and species of filamentous fungi.

Abstract translation: 提供多肽制剂的方法，所述多肽制剂具有减少的不期望的酶促侧活性的含量，所述方法包括使含有所需多肽（例如酶，药学活性或免疫活性多肽）的培养基在小于2的pH下经历一段时间 需要在保留所需多肽的活性的同时灭活侧活性。 该方法可用于提供乳凝固酶产物，包括基于凝乳酶或胃蛋白酶或微生物天冬氨酸蛋白酶的乳杆菌或凝血剂。 衍生自细菌种类和丝状真菌的种类。