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    INDUCIBLE DIMERIZATION OF RECOMBINASES
    3.
    发明申请
    INDUCIBLE DIMERIZATION OF RECOMBINASES 审中-公开

    公开(公告)号：WO2018111838A1

    公开(公告)日：2018-06-21

    申请号：PCT/US2017/065754

    申请日：2017-12-12

    申请人： TRUSTEES OF BOSTON UNIVERSITY

    发明人： WONG, Wilson W. ,  WEINBERG, Benjamin Harris

    IPC分类号： C07K14/705 ,  C07K19/00

    CPC分类号： C12N9/96 ,  C12N9/00

    摘要： The technology described herein relates to controlled chemically- or light-induced rejoinder of split-recombinases. In some embodiments, compositions, methods, kits and systems are provided that relate to a split-recombinase system, whereby protein complementation or rejoinder of split-recombinases is mediated by chemical-induced dimerization domains (CIDDs) or light-induced dimerization domains (LIDD) and rejoinder of the split-recombinases occur in the presence of one or more chemical inducers or light inducers, respectively. The split-recombinases systems as disclosed herein can be used in gene therapy, integrated logic and memory in living cells such as mammalian cells. The nucleic acid cassettes, switches, and systems described herein allow for controlled gene expression or gene regulation. The controlled chemically- or light-induced rejoinder of split-recombinases can be used in, for example, adopted T-cell therapy.

    PROCESS TO MANUFACTURE CROSS-LINKED ENZYME AGGREGATES
    9.
    发明申请
    PROCESS TO MANUFACTURE CROSS-LINKED ENZYME AGGREGATES 审中-公开
    制造交联酶的方法

    公开(公告)号：WO2017036916A1

    公开(公告)日：2017-03-09

    申请号：PCT/EP2016/070093

    申请日：2016-08-25

    申请人： UNILEVER N.V. ,  UNILEVER PLC ,  CONOPCO, INC., D/B/A UNILEVER

    发明人： DE ROSE, Simone, Antonio ,  DOWD, Andrew ,  LANG, Dietmar, Andreas ,  LITTLECHILD-BOND, Jennifer, Ann ,  NOVAK, Halina, Rose ,  PARRY, Neil, James ,  SINGH, Sukriti

    IPC分类号： C12N9/96 ,  C11D3/386

    CPC分类号： C11D3/38618 ,  C11D3/38627 ,  C11D3/38663 ,  C11D3/38681 ,  C12N9/96

    摘要： A process for the manufacture of cross-linked enzyme aggregates comprising the steps of: 1) providing a liquid mixture of the enzyme; 2) precipitating the enzyme comprised by the liquid mixture; 3) cross-linking the enzyme by addition of a cross-linking agent, having at least two reactive groups suitable for cross-linking amino acids; 4) stopping the cross-linking by addition of a suitable buffer; wherein step 2) is performed in the presence of a compound selected from a crown ether or a surfactant; and wherein step 4) is preferably performed when the mean diameter of the particles is at most 4.0 µm, as measured using a Turbiscan MA200 at 21 degrees Celsius, using the Stokes-Einstein equation to measure the particle size.

    摘要翻译： 一种制备交联酶聚集体的方法，包括以下步骤：1）提供酶的液体混合物; 2）沉淀由液体混合物组成的酶; 3）通过加入具有至少两个适于交联氨基酸的反应性基团的交联剂来交联酶; 4）加入合适的缓冲液停止交联; 其中步骤2）在选自冠醚或表面活性剂的化合物的存在下进行; 并且其中步骤4）优选在颗粒的平均直径为至多4.0μm时进行，如使用斯托克斯 - 爱因斯坦（Stokes-Einstein）方程测量粒径的方法，使用Turbiscan MA200在21摄氏度下测量。

    TETHERING CYSTEINE RESIDUES USING CYCLIC DISULFIDES
    10.
    发明申请
    TETHERING CYSTEINE RESIDUES USING CYCLIC DISULFIDES 审中-公开
    使用循环消除的CYCETINE残留物

    公开(公告)号：WO2017031226A1

    公开(公告)日：2017-02-23

    申请号：PCT/US2016/047395

    申请日：2016-08-17

    申请人： BRANDEIS UNIVERSITY

    发明人： AGAR, Jeffrey N. ,  SALISBURY, Joseph

    IPC分类号： A61K31/095 ,  A61K31/385 ,  C07K19/00 ,  C12N9/02 ,  C12N9/96

    CPC分类号： C12N9/0089 ,  A61K31/095 ,  A61K31/385 ,  C07K19/00 ,  C07K2319/00 ,  C12N9/96 ,  C12Y115/01001

    摘要： Described herein are compounds and methods for tethering proteins. For example, dimers of Protein X listed in Table 1 are described, where the dimers are formed by the covalent bonding of a cysteine on the first monomer to a cysteine on the second monomer via a cyclic disulfide linker. The covalently attached dimers exhibit increased stabilization and can be used to treat neurodegenerative diseases (such as, for example, Parkinson's Disease, ALS, Alzheimer's Disease, Huntington's Disease, Epilepsy, Frontotemporal Dementia, and/or DMD), cancer, autoimmune disease, and/or Celiac disease.

    摘要翻译： 本文描述的是用于结合蛋白质的化合物和方法。 例如，描述了表1中列出的蛋白X的二聚体，其中通过环二硫键连接体将第一单体上的半胱氨酸与第二单体上的半胱氨酸共价结合形成二聚体。 共价连接的二聚体表现出增加的稳定性，并且可用于治疗神经变性疾病（例如帕金森病，ALS，阿尔茨海默病，亨廷顿病，癫痫，额颞叶痴呆和/或DMD），癌症，自身免疫性疾病和 /或乳糜泻。