公开(公告)号：WO2014144270A1

公开(公告)日：2014-09-18

申请号：PCT/US2014/028604

申请日：2014-03-14

Applicant: HELIAE DEVELOPMENT, LLC

Inventor： TONKOVICH, Anna, Lee ,  LICAMELE, Jason ,  GANUZA, Eneko ,  GALVEZ, Adriano ,  SULLIVAN, Timothy, James ,  ADAME, Thomas ,  ESLER, James

IPC: C12M1/00 ,  C12M1/06 ,  C12M1/02

CPC classification number: C12M21/02 ,  C12M23/18 ,  C12M23/34 ,  C12M23/38 ,  C12M27/06 ,  C12M41/24 ,  C12M41/26

Abstract: Systems for culturing mixotrophic microorganisms on a large scale in thermally stable open systems are disclosed. Embodiments of the system include features such as lit portions, dark portions, organic carbon delivery systems, gas delivery systems, submersible thrusters for mixing, and turning vanes for guiding fluid flow. Multi-functional embodiments of the turning vane provide guidance for fluid flow and other functions such as heat exchange, nutrient delivery, gas delivery, organic carbon delivery, delivery of light, and parameter measurement by sensors.

Abstract translation: 公开了在热稳定的开放系统中大规模培养混合型微生物的系统。 该系统的实施例包括诸如点燃部分，暗部分，有机碳输送系统，气体输送系统，用于混合的潜水推进器和用于引导流体流动的转向叶片的特征。 转向叶片的多功能实施例为流体流动和其他功能提供指导，例如热交换，营养物质输送，气体输送，有机碳输送，光输送和传感器的参数测量。

公开(公告)号：WO2014074790A1

公开(公告)日：2014-05-15

申请号：PCT/US2013/069091

申请日：2013-11-08

Applicant: HELIAE DEVELOPMENT, LLC

Inventor： KALE, Aniket ,  GALVEZ, Adriano ,  KUHLMAN, Scott

IPC: C12N1/12 ,  A01G33/00 ,  B03C3/017 ,  C12M1/12 ,  C12M1/00 ,  C12N1/38 ,  C12N13/00

CPC classification number: C12N1/38 ,  A01G33/00 ,  B03C3/0175 ,  C12M37/00 ,  C12M47/02 ,  C12N1/12 ,  C12N13/00

Abstract: Methods of reducing a concentration of contaminating organisms in a culture of microorganisms using the application of electrical fields are described herein. The culture of microorganisms may also be concentrated simultaneously with the reduction in the concentration of contaminating organisms to form a substantially solids-rich fraction comprising coagulated microorganisms and separated from the substantially liquid fraction comprising contaminating organisms. The microorganisms resulting from the method may be packaged or inoculated in a culture medium for continued growth. The substantially liquid fraction may be further cleaned or purified for further use as a culture medium.

Abstract translation: 本文描述了使用电场施用来减少微生物培养物中污染生物浓度的方法。 微生物的培养物也可以与污染生物体的浓度降低同时浓缩，以形成基本上富含固体的级分，其包含凝固的微生物并从包含污染生物体的基本上液体的馏分中分离出来。 由该方法产生的微生物可以包装或接种在培养基中以继续生长。 可以进一步清洗或纯化基本上液体的部分作为培养基进一步使用。

公开(公告)号：WO2014074772A1

公开(公告)日：2014-05-15

申请号：PCT/US2013/069049

申请日：2013-11-08

Applicant: HELIAE DEVELOPMENT, LLC

Inventor： LICAMELE, Jason, David ,  GANUZA, Eneko ,  TONKOVICH, Anna, Lee ,  WYATT, Cameron, Miles ,  BELLEFEUILLE, Michael, Vincent ,  GALVEZ, Adriano ,  KULAGA, Thomas, John

IPC: C12N1/12 ,  C12N1/34 ,  C12N5/00 ,  C12M1/00

CPC classification number: C12N1/12 ,  C12N1/34 ,  C12Q3/00

Abstract: Methods of culturing microorganisms in combinations of phototrophic, mixotrophic, and heterotrophic culture conditions are disclosed. A culture of microorganisms may be transitioned between culture conditions over the life of a culture in various combinations, utilizing various conditions in a sequential manner to optimize the culture for growth or product accumulation.

Abstract translation: 公开了以光营养，混养营养和异养培养条件组合培养微生物的方法。 微生物培养物可以在培养物的整个生命周期中以各种组合在培养条件之间转变，利用各种条件以连续方式优化用于生长或产物积累的培养物。