公开(公告)号：WO2011127167A1

公开(公告)日：2011-10-13

申请号：PCT/US2011/031414

申请日：2011-04-06

Applicant: HELIAE DEVELOPMENT, LLC ,  KALE, Aniket

Inventor： KALE, Aniket

IPC: C12N1/02 ,  A23J1/00

CPC classification number: C11B1/10 ,  B01D11/04 ,  C11B1/108 ,  Y02E50/13

Abstract: A method of dewatering algae and recycling water therefrom is presented. A method of dewatering a wet algal cell culture includes removing liquid from an algal cell culture to obtain a wet algal biomass having a lower liquid content than the algal cell culture. At least a portion of the liquid removed from the algal cell culture is recycled for use in a different algal cell culture. The method includes adding a water miscible solvent set to the wet algal biomass and waiting an amount of time to permit algal cells of the algal biomass to gather and isolating at least a portion of the gathered algal cells from at least a portion of the solvent set and liquid of the wet algal biomass so that a dewatered algal biomass is generated. The dewatered algal biomass can be used to generated algal products such as biofuels and nutraceuticals.

Abstract translation: 提出了一种从藻类中脱水和循环利用水的方法。 对湿藻细胞培养物进行脱水的方法包括从藻类细胞培养物中除去液体以获得具有比藻细胞培养物低的液体含量的湿藻类生物质。 将从藻细胞培养物中除去的至少一部分液体再循环用于不同的藻类细胞培养物中。 该方法包括向湿藻类生物质中加入水混溶性溶剂组，并等待一段时间以允许藻类生物质的藻类细胞从溶剂组的至少一部分收集并分离聚集的藻类细胞的至少一部分 和湿藻类生物质的液体，从而产生脱水的藻类生物质。 脱水藻类生物质可用于生成藻类产品，如生物燃料和营养品。

公开(公告)号：WO2013142694A1

公开(公告)日：2013-09-26

申请号：PCT/US2013/033311

申请日：2013-03-21

Applicant: HELIAE DEVELOPMENT, LLC

Inventor： KALE, Aniket

IPC: B01D11/02 ,  C11B7/00

CPC classification number: B01D11/0234 ,  B01D11/0203 ,  B01D11/028 ,  B01D11/0288 ,  C11B1/10 ,  C11B1/108 ,  C11C3/003

Abstract: A method for separating neutral lipids from plant material, in particular, intact algal cells, using an amphipathic solvent set and a hydrophobic solvent set. Some embodiments include dewatering intact algal cells and then extracting neutral lipids from the algal cells. The methods provide for single and multistep extraction processes which allow for efficient separation of algal neutral lipids from a wet algal biomass while avoiding emulsification of extraction mixtures. These neutral lipids are high value products which can be used to generate renewable fuels.

Abstract translation: 使用两亲性溶剂组和疏水性溶剂组从植物材料，特别是完整的藻类细胞中分离中性脂质的方法。 一些实施方案包括将完整的藻类细胞脱水，然后从藻类细胞中提取中性脂质。 该方法提供单步和多步提取方法，其允许藻类中性脂质与湿藻类生物质的有效分离，同时避免提取混合物的乳化。 这些中性脂质是可用于生产可再生燃料的高价值产品。

公开(公告)号：WO2011127171A1

公开(公告)日：2011-10-13

申请号：PCT/US2011/031419

申请日：2011-04-06

Applicant: HELIAE DEVELOPMENT, LLC ,  KALE, Aniket

Inventor： KALE, Aniket

IPC: C11B1/10 ,  C12P7/64

CPC classification number: C11B1/10 ,  B01D11/04 ,  C11B1/108 ,  Y02E50/13

Abstract: A method for producing biofuels is provided. A method of making biofuels includes dewatering substantially intact algal cells to make an algal biomass, extracting neutral lipids from the algal biomass, and esterifying the neutral lipids with a catalyst in the presence of an alcohol. The method also includes separating a water soluble fraction comprising glycerin from a water insoluble fraction comprising fuel esters and distilling the fuel esters under vacuum to obtain a C 16 or shorter fuel esters fraction, a C16 or longer fuel ester fraction, and a residue comprising carotenoids and omega-3 fatty acids. The method further includes hydrogenating and deoxygenating at least one of (i) the C16 or shorter fuel esters to obtain a jet fuel blend stock and (ii) the C 16 or longer fuel esters to obtain a diesel blend stock.

Abstract translation: 提供生产生物燃料的方法。 制造生物燃料的方法包括使基本上完整的藻细胞脱水以制备藻类生物质，从藻类生物质中提取中性脂质，并在醇存在下用催化剂酯化中性脂质。 该方法还包括从包含燃料酯的水不溶性部分中分离包含甘油的水溶性馏分，并在真空下蒸馏燃料酯以获得C 16或更短的燃料酯馏分，C16或更长的燃料酯馏分，以及包含类胡萝卜素 和ω-3脂肪酸。 该方法还包括将（i）C16或更短的燃料酯中的至少一种氢化和脱氧以获得喷气燃料共混料和（ii）C 16或更长的燃料酯以获得柴油混合原料。

公开(公告)号：WO2011127169A1

公开(公告)日：2011-10-13

申请号：PCT/US2011/031417

申请日：2011-04-06

Applicant: HELIAE DEVELOPMENT, LLC ,  KALE, Aniket

Inventor： KALE, Aniket

IPC: C11B1/10 ,  C12P7/64

CPC classification number: C11B1/10 ,  B01D11/04 ,  C11B1/108 ,  Y02E50/13

Abstract: A method of isolating nutraceuticals products from algae is provided. A method of isolating carotenoids and omega-3 rich oil from algae includes dewatering substantially intact algal cells to make an algal biomass and adding a first ethanol fraction to the algal biomass. The method also includes separating a first substantially solid biomass fraction from a first substantially liquid fraction comprising proteins and combining the first substantially solid biomass fraction with a second ethanol fraction. The method further includes separating a second substantially solid biomass fraction from a second substantially liquid fraction comprising polar lipids and combining the second substantially solid biomass fraction with a third ethanol solvent fraction. The method also includes separating a third substantially solid biomass fraction from a third substantially liquid fraction comprising neutral lipids, wherein the third substantially solid biomass fraction comprises carbohydrates and separating the neutral lipids into carotenoids and omega-3 rich oil.

Abstract translation: 提供了一种从藻类中分离营养品的方法。 从藻类中分离类胡萝卜素和富含ω-3的油的方法包括将基本上完整的藻类细胞脱水以制备藻类生物质并向藻类生物质中加入第一乙醇级分。 该方法还包括从包含蛋白质的第一基本上液体的馏分中分离第一基本上固体的生物质馏分并将第一基本上固体的生物质馏分与第二乙醇馏分组合。 该方法还包括从包含极性脂质的第二基本上液体的部分中分离第二基本上固体的生物质馏分，并将第二基本上固体的生物质馏分与第三乙醇溶剂馏分组合。 该方法还包括从包含中性脂质的第三基本上液体的级分分离第三基本上固体的生物质馏分，其中第三基本上固体的生物质级分包含碳水化合物并将中性脂质分离成类胡萝卜素和富含ω3的油。

公开(公告)号：WO2012138382A1

公开(公告)日：2012-10-11

申请号：PCT/US2011/059152

申请日：2011-11-03

Applicant: HELIAE DEVELOPMENT, LLC ,  KALE, Aniket

Inventor： KALE, Aniket

IPC: C12R1/89 ,  C11B1/10 ,  C12P7/64

CPC classification number: C11B1/10 ,  C11B1/108 ,  C12P7/6463 ,  C12P7/649 ,  Y02E50/13

Abstract: A method for separating polar lipids from plant material, in particular, intact algal cells, using an amphipathic solvent set and a hydrophobic solvent set. Some embodiments include dewatering intact algal cells and then extracting polar lipids from the algal cells. The methods provide for single and multistep extraction processes which allow for efficient separation of algal polar lipids from a wet algal biomass while avoiding emulsification of extraction mixtures. These polar lipids are high value products which can be used as surfactants, detergents, and food additives. Neutral lipids remaining in the algal biomass after extraction of polar lipids can be used to generate renewable fuels.

Abstract translation: 使用两亲性溶剂组和疏水性溶剂组从植物材料，特别是完整的藻类细胞中分离极性脂质的方法。 一些实施方案包括使完整的藻细胞脱水，然后从藻类细胞中提取极性脂质。 该方法提供单步和多步提取方法，其允许藻类极性脂质与湿藻类生物质的有效分离，同时避免提取混合物的乳化。 这些极性脂质是可用作表面活性剂，洗涤剂和食品添加剂的高价值产品。 提取极性脂质后残留在藻类生物质中的中性脂质可用于产生可再生燃料。

公开(公告)号：WO2012138381A1

公开(公告)日：2012-10-11

申请号：PCT/US2011/059148

申请日：2011-11-03

Applicant: HELIAE DEVELOPMENT, LLC ,  KALE, Aniket

Inventor： KALE, Aniket

IPC: C12R1/89 ,  C11B1/10 ,  C12P7/64 ,  C07K1/14

CPC classification number: C11B1/10 ,  C07K1/145 ,  C11B1/108 ,  C11C3/003 ,  C12M21/02 ,  C12M47/10 ,  C12N1/06

Abstract: A method for separating proteins from plant material, in particular, intact algal cells, using an amphipathic solvent set and a hydrophobic solvent set. Some embodiments include dewatering intact algal cells and then extracting proteins from the algal cells. The methods provide for single and multistep extraction processes which allow for efficient separation of algal proteins from a wet algal biomass. These proteins are high value products which can be used as renewable sources of food and food additives. Neutral lipids remaining in the algal biomass after extraction of proteins can be used to generate renewable fuels.

Abstract translation: 使用两亲性溶剂组和疏水性溶剂组从植物材料，特别是完整藻细胞中分离蛋白质的方法。 一些实施方案包括将完整的藻细胞脱水，然后从藻细胞中提取蛋白质。 该方法提供了单步和多步提取方法，其允许藻类蛋白质与湿藻类生物质的有效分离。 这些蛋白质是可以用作食品和食品添加剂的可再生资源的高价值产品。 提取蛋白质后残留在藻类生物质中的中性脂质可用于产生可再生燃料。

公开(公告)号：WO2011127161A1

公开(公告)日：2011-10-13

申请号：PCT/US2011/031408

申请日：2011-04-06

Applicant: HELIAE DEVELOPMENT, LLC ,  KALE, Aniket

Inventor： KALE, Aniket

IPC: A23J1/00

CPC classification number: C11B1/10 ,  B01D11/04 ,  C11B1/108 ,  Y02E50/13

Abstract: Methods for selective extraction and fractionation of algal proteins from an algal biomass or algal culture are disclosed. A method of selective removal of products from an algal biomass provides for single and multistep extraction processes which allow for efficient separation of algal proteins. These proteins can be used as renewable sources of proteins for animal feedstocks and human food. Further, lipids remaining in the algal biomass after extraction of proteins can be used to generate renewable fuels.

Abstract translation: 公开了从藻类生物质或藻类培养物中选择性提取和分离藻类蛋白的方法。 从藻类生物质中选择性地除去产物的方法提供允许有效分离藻类蛋白质的单步骤和多步萃取方法。 这些蛋白质可用作动物原料和人类食物的可再生来源蛋白质。 此外，在提取蛋白质之后残留在藻类生物质中的脂质可用于产生可再生燃料。

公开(公告)号：WO2011127160A1

公开(公告)日：2011-10-13

申请号：PCT/US2011/031407

申请日：2011-04-06

Applicant: HELIAE DEVELOPMENT, LLC ,  KALE, Aniket

Inventor： KALE, Aniket

IPC: A23J1/00

CPC classification number: C11B1/10 ,  B01D11/04 ,  C11B1/108 ,  Y02E50/13

Abstract: Methods for selective extraction and fractionation of algal proteins from an algal biomass or algal culture are disclosed. A method of selective removal of products from an algal biomass provides for single and multistep extraction processes which allow for efficient separation of algal proteins. These proteins can be used as renewable sources of proteins for animal feedstocks and human food. Further, lipids remaining in the algal biomass after extraction of proteins can be used to generate renewable fuels.

Abstract translation: 公开了从藻类生物质或藻类培养物中选择性提取和分离藻类蛋白的方法。 从藻类生物质中选择性地除去产物的方法提供允许有效分离藻类蛋白质的单步骤和多步萃取方法。 这些蛋白质可用作动物原料和人类食物的可再生来源蛋白质。 此外，在提取蛋白质之后残留在藻类生物质中的脂质可用于产生可再生燃料。

公开(公告)号：WO2014011869A3

公开(公告)日：2014-01-16

申请号：PCT/US2013/050064

申请日：2013-07-11

Applicant: HELIAE DEVELOPMENT, LLC

Inventor： KNIEP, Justin, S. ,  KALE, Aniket

IPC: C12M1/00 ,  B01D21/00

Abstract: Described herein are systems, methods, and apparatuses for aggregating microorganism in an aqueous suspension. In particular, are systems, methods, and apparatuses that apply an electrical field and/or acoustic energy to an aqueous suspension comprising microorganisms as the aqueous suspension follows a flow path to cause aggregation of the microorganisms. The electrical field may be continuous or pulsed. In some embodiments, the flow path for the aqueous suspension may vary.

公开(公告)号：WO2013075116A3

公开(公告)日：2013-05-23

申请号：PCT/US2012/065889

申请日：2012-11-19

Applicant: HELIAE DEVELOPMENT, LLC

Inventor： SHINDE, Sandip ,  KALE, Aniket ,  KULAGA, Tom ,  LICAMELE, Jason, D. ,  TONKOVICH, Anna, Lee

IPC: A23D9/00 ,  A23L1/30 ,  A23L2/52 ,  A23D9/02 ,  A23K1/16 ,  A23L1/03 ,  A61K8/92 ,  C11B3/12 ,  C11B7/00 ,  C11C3/02 ,  C11B1/10

Abstract: Disclosed here are compositions rich in omega-7 fatty acids, including palmitoleic acid, and products rich in omega-7 fatty acids derived from algal biomass. The algae and/or compositions rich in omega-7 fatty acids may be used in products or as ingredients of products. Methods and systems for increasing the production or concentration of omega-7 fatty acids, and isolating omega-7 fatty acids from algal biomass are also disclosed herein.