公开(公告)号：WO1985002628A1

公开(公告)日：1985-06-20

申请号：PCT/US1984002024

申请日：1984-12-07

Applicant: HRI RESEARCH, INC.

Inventor： HRI RESEARCH, INC. ,  YABUSAKI, Kenichi, Ken ,  ISAACS, Stephen, T. ,  GAMPER, Howard, B., Jr.

IPC: C12Q01/68

CPC classification number: C12Q1/6816 ,  C12Q2563/101 ,  C12Q2523/101

Abstract: Novel methods and reagents for determining the presence of specific nucleic acid base sequences by employing crosslinking reactions of unique molecules capable of forming covalent bonds which are bonded with various labels or ligands for amplication. Single stranded nucleic acid probes are employed which contain complementary base sequences to nucleic acid target molecules. By first hybridizing and then forming covalent bonds between the probe and target the amount of label in the crosslinked hybrid can be measured as an extremely sensitive method for assaying for specific nucleic acid sequences.

公开(公告)号：WO1992008807A1

公开(公告)日：1992-05-29

申请号：PCT/US1991008492

申请日：1991-11-13

Applicant: HRI RESEARCH, INC.

Inventor： HRI RESEARCH, INC. ,  LIN, Lily ,  ISAACS, Stephen, T. ,  HEARST, John, E.

IPC: C12Q01/68

CPC classification number: C12Q1/703 ,  C07K16/44 ,  C12N15/1003 ,  C12Q1/6806 ,  C12Q1/6848 ,  C12Q2531/113 ,  C12Q2527/125 ,  C12Q2521/537

Abstract: Methods and compounds for the preparation of nucleic acid samples. Polymerase inhibitors are treated so as to allow for amplification of nucleic acid from biological sources, including whole blood.