公开(公告)号：WO2020102521A1

公开(公告)日：2020-05-22

申请号：PCT/US2019/061461

申请日：2019-11-14

Applicant: ILLUMINA, INC.

Inventor： CRIVELLI, Paul ,  DELATTRE, Cyril

IPC: B01L3/00 ,  B29C53/06 ,  G01N35/10

Abstract: An apparatus includes a fluid reservoir and a laminate fluidic circuit positioned above the fluid reservoir. The laminate fluidic circuit includes two or more layers laminated together to define a substantially planar substrate and one or more channels defined within the substrate. The laminate fluidic circuit includes a flexible conduit defined by a portion of the substrate encompassing an extent of at least one of the channels that is partially separated or separable from the remainder of the substrate. The flexible conduit is deflectable with respect to the planar substrate toward the fluid reservoir such that the flexible conduit fluidly connects the at least one channel to the fluid reservoir.

公开(公告)号：WO2019147419A1

公开(公告)日：2019-08-01

申请号：PCT/US2019/013002

申请日：2019-01-10

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： COX-MURANAMI, Wesley ,  SEGALE, Darren, Robert ,  MILLER, Oliver, Jon ,  FOLEY, Jennifer, Olivia ,  KHURANA, Tarun Kumar ,  CRIVELLI, Paul ,  WATTS, Gary

IPC: G01N35/08 ,  G01N35/02 ,  G01N37/00

Abstract: An apparatus for directing fluid into and out of a fluidic device includes two or more fluid prime channels connected to a fluid inlet of the fluidic device, a flow control valve for each fluid prime channel to control flow between the fluid prime channel and the fluid inlet, one or more outlet channels connected to a fluid outlet of the fluidic device, and a flow control valve for each outlet channel to control flow between the fluid outlet and the associated outlet channel. An apparatus for delivering fluids to a fluid inlet includes a plate that is rotatable about an axis of rotation and a plurality of fluid compartments disposed on the plate, each compartment having a fluid exit port disposed at a common radial distance from the axis of rotation and positioned to align with the fluid inlet as the plate rotates about the axis of rotation.

公开(公告)号：WO2022178542A1

公开(公告)日：2022-08-25

申请号：PCT/US2022/070738

申请日：2022-02-18

Applicant: ILLUMINA, INC.

Inventor： ZIEBARTH, Jonathan ,  ADAY, Jon ,  CRIVELLI, Paul ,  KRIENDL, Gerald ,  SHARMA, Amit

IPC: B01L3/00 ,  G01N21/64

Abstract: Provided herein include various examples of a method for manufacturing aspects of flow cell. The method may include performing chemical processes on a surface of the patterned wafer to prepare the surface of the patterned, singulating the wafer into individual dies, orienting each die on a temporary substrate, where the orienting creates spaces between each individual die, and molding a material over the spaces to create a hybrid wafer comprised of glass and molded material. The method may also include bonding two of the hybrid wafers together, forming a bonded wafer stack.

公开(公告)号：WO2021262366A1

公开(公告)日：2021-12-30

申请号：PCT/US2021/033879

申请日：2021-05-24

Applicant: ILLUMINA, INC.

Inventor： BECKMAN, Travis ,  CRIVELLI, Paul ,  COX-MURANAMI, Wesley ,  FOLEY, Jennifer ,  SEGALE, Darren ,  DUCHNAK, Allison ,  FRANKIE, Keith ,  MAR, Cameron

IPC: B01L3/00

Abstract: Dry reagent cup assemblies and methods are disclosed. In accordance with an implementation, an apparatus includes a liquid reservoir and dry reagent cup assembly. The liquid reservoir has a base, side wall that extends from the base, and distal opening. The dry reagent cup assembly coupled to the liquid reservoir includes a dry reagent cup and liquid impermeable barrier. The dry reagent cup has a cup base, cup side wall that extends from the cup base, and cup opening. The distal opening of the liquid reservoir faces the cup opening. The liquid impermeable barrier covers the cup opening and separates the liquid reservoir and the dry reagent cup. The dry reagent cup moves between an initial position outside the liquid reservoir and a rehydrating position where the dry reagent cup pierces and passes through an opening in the liquid impermeable barrier and is received within the liquid reservoir.

公开(公告)号：WO2019221913A1

公开(公告)日：2019-11-21

申请号：PCT/US2019/030186

申请日：2019-05-01

Applicant: ILLUMINA, INC.

Inventor： DELATTRE, Cyril ,  RHEE, Minsoung ,  LIU, Jeffrey ,  COX-MURANAMI, Wesley ,  CRIVELLI, Paul ,  FOLEY, Jennifer ,  SEGALE, Darren ,  TAYLOR, Jay ,  HAGE, Matthew ,  PAIK, Philip ,  ALLEGOREN, Erik ,  HERTZOG, David ,  MOROZ-SMIETANA, Alex ,  MA, Xiaoxiao ,  TAKAHASHI, Tsukasa ,  WESTERBERG, Brandon

IPC: G01N35/02 ,  B81B3/00

Abstract: An instrument includes a reagent management system. The reagent management system includes a plurality of reagent wells, each reagent well operable to contain a reagent of a plurality of reagents positioned therein. The reagent management system is operable to select a flow of reagent from one of the plurality of reagents. A flexible connection includes a laminate stack and includes a first flexible channel in fluid communication with the reagent management system. The first flexible channel is operable to route the flow of reagent therethrough. A flow cell includes a flow channel in fluid communication with the first flexible channel. The flow channel is operable to route the flow of reagent over analytes positioned in the flow channel. The flexible connection enables the flow cell to be moved by the instrument relative to a fixed reference point in the instrument.

公开(公告)号：WO2022197916A1

公开(公告)日：2022-09-22

申请号：PCT/US2022/020735

申请日：2022-03-17

Applicant: ILLUMINA, INC.

Inventor： PONKALA, Mikko ,  LIMB, Paul ,  SCHOCH, Reto ,  PHAM, Sonny ,  PATEL, Maulik ,  CRIVELLI, Paul

IPC: G01N35/10 ,  G01N35/00

Abstract: Non-contact dispenser assemblies and related systems and methods are disclosed. An implementation of an apparatus is disclosed that includes a reagent cartridge including a body defining a plurality of reservoirs. Each reservoir has an outlet and a distal end defining an opening, a manifold assembly including an outlet, a common fluidic line fluidly coupled to the outlet, a plurality of reagent fluidic lines coupled to the corresponding outlet of the reservoirs, and a plurality of membrane valves selectively fluidly coupling the common fluidic line and a corresponding one of the plurality of reagent fluidic lines.

公开(公告)号：WO2022192035A1

公开(公告)日：2022-09-15

申请号：PCT/US2022/018352

申请日：2022-03-01

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： CRIVELLI, Paul ,  DAVIDSON, Justin ,  RICOULT, Sebastien

IPC: E21B49/00 ,  E21B47/017 ,  E21B47/113 ,  E21B43/12 ,  E21B34/06

Abstract: Well assemblies enabling optical access therein and related systems and methods are disclosed. In accordance with an implementation, an apparatus includes a body, dry reagent, and a cover. The body defines a well and has an opening, an aperture, and a field of view (FOV) enabling optical access from the aperture to the well. The dry reagent is contained within the well. The cover is coupled to the body and covering the opening.

公开(公告)号：WO2022119744A1

公开(公告)日：2022-06-09

申请号：PCT/US2021/060706

申请日：2021-11-24

Applicant: ILLUMINA, INC.

Inventor： MAR, Cameron ,  TAHERI, Payam ,  DELATTRE, Cyril ,  CRIVELLI, Paul ,  COX-MURANAMI, Wesley ,  CAO, Zhenning ,  BECKMAN, Travis ,  DAVIDSON, Justin

IPC: B01L3/00

Abstract: Well assemblies and related methods are disclosed. In accordance with an implementation, an apparatus includes a liquid reservoir containing a liquid and a well assembly including a body, a hydrophobic venting membrane, and a cover. The body defines a well and has an opening and a port. The port is couplable to the liquid reservoir. The hydrophobic venting membrane is coupled to the body and covers the opening and cover covers the hydrophobic venting membrane. As the liquid is flowed into the well via the port, the hydrophobic venting membrane vents gas contained within the well.

公开(公告)号：WO2020070664A1

公开(公告)日：2020-04-09

申请号：PCT/IB2019/058382

申请日：2019-10-02

Applicant: ILLUMINA, INC

Inventor： COX-MURANAMI, Wesley ,  OSMUS, James ,  CRIVELLI, Paul ,  DREWS, Bradley Kent

IPC: B01L3/00

Abstract: An apparatus includes a fluidic circuit, a bypass fluidic circuit, a first set of fluid wells, a second set of fluid wells, a first valve, and a second valve. The first valve operatively associated with the first set of fluid wells such that the first selectively fluidly connects any one of the first set of fluid wells to a first valve outlet. The second valve operatively associated with the fluidic circuit, the bypass fluidic circuit, the first valve outlet, and the second set of fluid wells such that the second valve selectively fluidly connects any one of the second set of fluid wells and the first valve outlet to the fluidic circuit or the first valve outlet to the bypass fluidic circuit.

公开(公告)号：WO2019152677A1

公开(公告)日：2019-08-08

申请号：PCT/US2019/016097

申请日：2019-01-31

Applicant: ILLUMINA, INC.

Inventor： SEGALE, Darren ,  TRAN, Hai ,  CRIVELLI, Paul

IPC: B01L3/00

Abstract: A circuit with electrical interconnect for external electronic connection and sensor (s) on a die are combined with a laminated manifold to deliver a liquid reagent over an active surface of the sensor (s). The laminated manifold includes fluidic channel (s), an interface between the die and the fluidic channel (s) being sealed. Also disclosed is a method, the method including assembling a laminated manifold including fluidic channel (s), attaching sensor (s) on a die to a circuit, the circuit including an electrical interconnect, and attaching a planarization layer to the circuit, the planarization layer including a cut out for the die. The method further includes placing sealing adhesive at sides of the die, attaching the laminated manifold to the circuit, and sealing an interface between the die and fluidic channel (s).