公开(公告)号：WO2015160895A2

公开(公告)日：2015-10-22

申请号：PCT/US2015/025889

申请日：2015-04-15

Applicant: ILLUMINA, INC.

Inventor： GLOECKNER, Christian ,  KIA, Amirali ,  BOMATI, Erin ,  HE, Molly ,  GRUNENWALD, Haiying Li ,  KUERSTEN, Scott ,  OSOTHPRAROP, Trina Faye ,  HASKINS, Darin ,  BURGESS, Joshua ,  KHANNA, Anupama ,  SCHLINGMAN, Daniel ,  VAIDYANATHAN, Ramesh

IPC: C12N9/22

CPC classification number: C12N9/1241 ,  C12N9/22 ,  C12N15/1068 ,  C12Q1/6869 ,  C12Q2521/507 ,  C12Q2565/514

Abstract: Presented herein are transposase enzymes and reaction conditions for improved fragmentation and tagging of nucleic acid samples, in particular altered transposases and reaction conditions which exhibit improved insertion sequence bias, as well as methods and kits using the same.

Abstract translation: 本文提出的是用于改进核酸样品的片段化和标记的转座酶和反应条件，特别是显示出改进的插入序列偏差的改变的转座酶和反应条件，以及使用其的方法和试剂盒。