公开(公告)号：WO2016073690A1

公开(公告)日：2016-05-12

申请号：PCT/US2015/059194

申请日：2015-11-05

Applicant: ILLUMINA, INC.

Inventor： GLOECKNER, Christian ,  KIA, Amirali ,  HE, Molly ,  OSOTHPRAROP, Trina Faye ,  STEEMERS, Frank, J. ,  GUNDERSON, Kevin, L. ,  AMINI, Sasan ,  JENDRISAK, Jerome

IPC: C12N15/10 ,  C12N9/00 ,  C12N9/22

CPC classification number: C12N15/1065 ,  C12N9/1241 ,  C12N15/1093 ,  C12Q2563/185 ,  C12Y207/07

Abstract: Presented herein are methods and compositions for tagmentation of nucleic acids. The methods are useful for generating tagged DNA fragments that are qualitatively and quantitatively representative of the target nucleic acids in the sample from which they are generated.

Abstract translation: 本文提出的是用于标记核酸的方法和组合物。 该方法可用于产生定性和定量代表其产生的样品中的靶核酸的标记DNA片段。

公开(公告)号：WO2016073690A9

公开(公告)日：2016-05-12

申请号：PCT/US2015/059194

申请日：2015-11-05

Applicant: ILLUMINA, INC.

Inventor： GLOECKNER, Christian ,  KIA, Amirali ,  HE, Molly ,  OSOTHPRAROP, Trina Faye ,  STEEMERS, Frank, J. ,  GUNDERSON, Kevin, L. ,  AMINI, Sasan ,  JENDRISAK, Jerome

IPC: C12N15/10 ,  C12N9/00 ,  C12N9/22

Abstract: Presented herein are methods and compositions for tagmentation of nucleic acids. The methods are useful for generating tagged DNA fragments that are qualitatively and quantitatively representative of the target nucleic acids in the sample from which they are generated.

公开(公告)号：WO2015160895A2

公开(公告)日：2015-10-22

申请号：PCT/US2015/025889

申请日：2015-04-15

Applicant: ILLUMINA, INC.

Inventor： GLOECKNER, Christian ,  KIA, Amirali ,  BOMATI, Erin ,  HE, Molly ,  GRUNENWALD, Haiying Li ,  KUERSTEN, Scott ,  OSOTHPRAROP, Trina Faye ,  HASKINS, Darin ,  BURGESS, Joshua ,  KHANNA, Anupama ,  SCHLINGMAN, Daniel ,  VAIDYANATHAN, Ramesh

IPC: C12N9/22

CPC classification number: C12N9/1241 ,  C12N9/22 ,  C12N15/1068 ,  C12Q1/6869 ,  C12Q2521/507 ,  C12Q2565/514

Abstract: Presented herein are transposase enzymes and reaction conditions for improved fragmentation and tagging of nucleic acid samples, in particular altered transposases and reaction conditions which exhibit improved insertion sequence bias, as well as methods and kits using the same.

Abstract translation: 本文提出的是用于改进核酸样品的片段化和标记的转座酶和反应条件，特别是显示出改进的插入序列偏差的改变的转座酶和反应条件，以及使用其的方法和试剂盒。