公开(公告)号：WO2016067306A1

公开(公告)日：2016-05-06

申请号：PCT/IN2015/000404

申请日：2015-10-30

Applicant: INDIAN COUNCIL OF MEDICAL RESEARCH

Inventor： YERNENI, Lakshmana Kumar ,  Chugh, Man Rishi

IPC: C12N5/071

CPC classification number: C12N5/063 ,  C12N2501/999 ,  C12N2502/1323

Abstract: This invention relates to a culture system for the growth of epidermal keratinocyte stem cells comprising, a. processing the feeder cells economically with Mitomycin C; and b. providing a substratum in the form of such feeder cells; c. achieving better growth stimulation of epidermal keratinocyte stem cell growth than γ-irradiated feeder cells; characterized in that the optimal concentration of mitomycin C is combined further with respect to the dose per cell to derive a range of concentration-dose permutations, where the concentration of mitomycin C is in the range of 3 to 10 μg per ml and the dose is in the range of 15 to 450 pg per cell.

Abstract translation: 本发明涉及用于生长表皮角化细胞干细胞的培养系统，其包括：a。 用丝裂霉素C经济地加工饲养细胞; 和b。 提供这种饲养细胞形式的基质; C。 与γ-照射的饲养细胞相比，实现表皮角质细胞干细胞生长的更好的生长刺激; 其特征在于，丝裂霉素C的最佳浓度进一步与每个细胞的剂量组合以得出浓度 - 剂量排列的范围，其中丝裂霉素C的浓度在3至10μg/ ml范围内，并且剂量为 在每个细胞15至450pg的范围内。