公开(公告)号：WO2015001570A1

公开(公告)日：2015-01-08

申请号：PCT/IN2014/000441

申请日：2014-07-02

Applicant: INDIAN COUNCIL OF MEDICAL RESEARCH

Inventor： PARASHAR, DEEPTI ,  ARANKALLE, A. , VIDYA ,  PAINGANKAR, S., MANDAR ,  KUMAR, SATYENDRA ,  GOKHALE, D., MANGESH ,  SUDEEP, B., A. ,  SHINDE, S., SAPNA

IPC: C12N15/113 ,  A61P31/14 ,  A61K31/713

CPC classification number: C12N15/1131 ,  A61K31/713 ,  C12N2310/14 ,  C12N2320/30

Abstract: The present invention relates to RNAi agents for inhibition of Chikungunya virus are described herein. The present invention provides RNAi agents for inhibition of Chikungunya virus, particularly by targeting the E2 gene and nsPl gene or both of the Chikungunya virus; the RNAi agents comprising of the entire nucleotide sequence set forth is SEQ ID 1 or SEQ ID 5 or combination thereof; or comprising of 15 or more contiguous nucleotides as set forth is SEQ ID 1 or SEQ ID 5 or combination thereof along with the addition nucleotides from the contiguous region of the E2 and nsPl target gene. The invention further provides a RNAi composition for reducing the E2 protein and nsPl protein level of Chikungunya virus and inhibition of Chikungunya virus replication. The combination of RNAi agents provides an excellent therapeutic composition for treatment of Chikungunya virus infection.

Abstract translation: 本发明涉及用于抑制基孔肯雅病毒的RNAi剂。 本发明提供用于抑制基孔肯雅病毒的RNAi试剂，特别是通过靶向E2基因和nsP1基因或两者基孔肯雅病毒; 包含所述全部核苷酸序列的RNAi试剂是SEQ ID 1或SEQ ID 5或其组合; 或包含15个或更多个连续核苷酸，如SEQ ID 1或SEQ ID 5或其组合以及来自E2和nsP1靶基因的连续区域的加成核苷酸。 本发明还提供了用于降低基孔肯雅病毒的E2蛋白和nsP1蛋白水平的RNAi组合物，并抑制基孔肯雅病毒复制。 RNAi剂的组合提供了治疗基孔肯雅病毒感染的极好的治疗组合物。