公开(公告)号：WO2011136602A9

公开(公告)日：2012-03-01

申请号：PCT/KR2011003189

申请日：2011-04-29

Applicant: KOREA RES INST OF BIOSCIENCE ,  LEE EUN GYO ,  LEE HONG WEON ,  KIM YEON GU ,  JUNG JOON KI ,  AHN JUNG OH ,  KIM CHUN SUG ,  LEE HYEOK WON ,  LEE JOO HWAN ,  LEE HYE LYM

Inventor： LEE EUN GYO ,  LEE HONG WEON ,  KIM YEON GU ,  JUNG JOON KI ,  AHN JUNG OH ,  KIM CHUN SUG ,  LEE HYEOK WON ,  LEE JOO HWAN ,  LEE HYE LYM

IPC: C12N15/64 ,  C12N15/13 ,  C12N15/65 ,  C12Q1/02 ,  G01N33/52

CPC classification number: G01N33/536 ,  G01N33/542

Abstract: The present invention relates to a method for selecting an antibody-producing cell line by using a split fluorescent protein, and a kit for selecting an antibody-producing cell line. The use of a split fluorescent protein in selection of an antibody-producing cell line according to the present invention enables easy detection of an antibody-producing cell line just with observing the expression of a single fluorescent color by recombination of a split fluorescent protein, thereby remarkably reducing the time and cost for selecting an antibody-producing cell line with high productivity.

Abstract translation: 本发明涉及通过使用分裂荧光蛋白质选择产生抗体的细胞系的方法和用于选择抗体产生细胞系的试剂盒。 根据本发明，使用分裂的荧光蛋白选择产生抗体的细胞系，只要通过分裂荧光蛋白的重组观察单一荧光颜色的表达，就可以容易地检测抗体产生细胞系，由此 显着降低了以高生产率选择产生抗体的细胞系的时间和成本。

公开(公告)号：WO2011136602A3

公开(公告)日：2012-04-19

申请号：PCT/KR2011003189

申请日：2011-04-29

Applicant: KOREA RES INST OF BIOSCIENCE ,  LEE EUN GYO ,  LEE HONG WEON ,  KIM YEON GU ,  JUNG JOON KI ,  AHN JUNG OH ,  KIM CHUN SUG ,  LEE HYEOK WON ,  LEE JOO HWAN ,  LEE HYE LYM

Inventor： LEE EUN GYO ,  LEE HONG WEON ,  KIM YEON GU ,  JUNG JOON KI ,  AHN JUNG OH ,  KIM CHUN SUG ,  LEE HYEOK WON ,  LEE JOO HWAN ,  LEE HYE LYM

IPC: C12N15/64 ,  C12N15/13 ,  C12N15/65 ,  C12Q1/02 ,  G01N33/52

CPC classification number: G01N33/536 ,  G01N33/542

Abstract: The present invention relates to a method for selecting an antibody-producing cell line by using a split fluorescent protein, and a kit for selecting an antibody-producing cell line. The use of a split fluorescent protein in selection of an antibody-producing cell line according to the present invention enables easy detection of an antibody-producing cell line just with observing the expression of a single fluorescent color by recombination of a split fluorescent protein, thereby remarkably reducing the time and cost for selecting an antibody-producing cell line with high productivity.

Abstract translation: 本发明涉及通过使用裂解荧光蛋白来选择产生抗体的细胞系的方法，以及用于选择抗体产生细胞系的试剂盒。 根据本发明，在选择产生抗体的细胞系中使用裂解荧光蛋白，只需通过重组分裂荧光蛋白观察单一荧光颜色的表达，就能够容易地检测产生抗体的细胞系，由此 显着降低了选择生产抗体的细胞系的时间和成本。

公开(公告)号：WO2014182016A9

公开(公告)日：2015-09-24

申请号：PCT/KR2014003933

申请日：2014-05-02

Applicant: KOREA RES INST OF BIOSCIENCE

Inventor： LEE HONG-WEON ,  AHN JUNGOH ,  JUNG JOON KI ,  KO HEE-JU ,  PARK SUN-JOO ,  KIM CHUN SUG ,  LEE HYEOK WON ,  LEE EUN GYO ,  LEE JOO HWAN

IPC: C12N1/21 ,  C12N15/52 ,  C12P7/44

CPC classification number: C12N15/52 ,  C12P13/005 ,  C12P17/10

Abstract: The present invention relates to a method for preparing a recombinant microorganism simultaneously comprising genes encoding enzymes used for a biosynthesis pathway of 6-aminocaproic acid which is a precursor of caprolactam, biosynthesizing 6-aminocaproic acid from the microorganism, and producing same so as to synthesize caprolactam.

Abstract translation: 本发明涉及同时制备重组微生物的方法，该方法同时包含编码用作己内酰胺前体的6-氨基己酸的生物合成途径的酶的基因，从微生物中合成6-氨基己酸，并生成合成 己内酰胺。