公开(公告)号：WO2016061592A1

公开(公告)日：2016-04-21

申请号：PCT/US2015/056288

申请日：2015-10-19

Applicant: LABCYTE INC.

Inventor： ELLSON, Richard N. ,  STEARNS, Richard G. ,  OLECHNO, Joseph D. ,  SINCLAIR, Ian ,  WINGFIELD, Jonathan

IPC: G01N33/543 ,  C12Q1/68 ,  G01N21/64

CPC classification number: G01N1/28 ,  C12Q1/68 ,  G01N33/6848 ,  G01N33/6851 ,  H01J49/04 ,  H01J49/0422 ,  H01J49/0431 ,  Y10T436/24 ,  Y10T436/25875

Abstract: A method and system are provided for detecting the concentration of an anaiyte in a fluid sample. The method and system involve analysis of a volatilized, ionized fluid sample using a mass spectrometer or other ionic anaiyte detection device that provides a signal proportional in intensity to the quantity of ionized anaiyte detected. The improvement involves replacement of a necessary non-analyte component in the fluid sample with a substitute component that serves the same purpose as the original component but is either more volatile than the original component and/or the anaiyte or undergoes a reaction to provide lower molecular weight reaction products, and results in an increased intensity in signal and signal-to-noise ratio. Acoustic fluid ejection is a preferred method of generating nanoliter-sized droplets of fluid sample that are then volatilized, ionized, and analyzed.

Abstract translation: 提供了一种用于检测流体样品中厌氧剂浓度的方法和系统。 该方法和系统涉及使用质谱仪或其它离子电解质检测装置对挥发的离子化流体样品进行分析，其提供了与所检测的电离量的强度成正比的信号。 该改进涉及用具有与原始组分相同目的的替代组分替换流体样品中必需的非分析物组分，但是比原始组分和/或非离子组分更易挥发或者经历反应以提供较低分子量 重量反应产物，并导致信号和信噪比的强度增加。 声流体喷射是产生流体样品的纳升级大小的液滴的优选方法，然后将其挥发，电离和分析。