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    Small RNA Capture, Detection and Quantification
    2.
    发明申请
    Small RNA Capture, Detection and Quantification 审中-公开
    小RNA捕获，检测和定量

    公开(公告)号：WO2014071322A1

    公开(公告)日：2014-05-08

    申请号：PCT/US2013/068350

    申请日：2013-11-04

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： WONG, Linda ,  CHEN, Caifu ,  WU, Yalei ,  DONG, Shoulian ,  LIU, Chunmei

    IPC: C12Q1/68

    CPC classification number: C12Q1/6855 ,  C12P19/34 ,  C12Q1/6832 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q2525/191 ,  C12Q2525/301

    Abstract: Methods, compositions and kits for capturing, detecting and quantifying mature small RNAs are provided herein. Embodiments of the methods comprise ligating 5' and 3' ligation adaptors to the 5' and 3' ends of the mature small RNAs, respectively, in the presence of 5' and 3' semi-degenerate ligation splints to generate a ligation product. Other embodiments comprise reverse transcribing polyadenylated mature small RNA with a universal reverse transcription primer and ligating an adaptor to the 3' end of the cDNA in the presence of a semi-degenerate ligation splint to generate a cDNA lligation product.

    Abstract translation: 本文提供了用于捕获，检测和定量成熟小RNA的方法，组合物和试剂盒。 方法的实施方案包括在5'和3'半简并连接夹板的存在下分别将5'和3'连接衔接子连接到成熟小RNA的5'和3'末端以产生连接产物。 其他实施方案包括具有通用逆转录引物的逆转录多聚腺苷酸成熟小RNA，并且在半退化连接夹板存在下将衔接子连接到cDNA的3'末端以产生cDNA连接产物。

    METHODS, COMPOSITIONS AND KITS FOR SMALL RNA CAPTURE, DETECTION AND QUANTIFICATION
    3.
    发明申请
    METHODS, COMPOSITIONS AND KITS FOR SMALL RNA CAPTURE, DETECTION AND QUANTIFICATION 审中-公开
    方法，小RNA捕获，检测和定量的组合物和试剂盒

    公开(公告)号：WO2016149021A1

    公开(公告)日：2016-09-22

    申请号：PCT/US2016/021679

    申请日：2016-03-10

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： LIU, Chunmei ,  DONG, Shoulian ,  WONG, Linda

    IPC: C12Q1/68

    CPC classification number: C12Q1/686 ,  C12Q1/6806 ,  C12Q1/6851 ,  C12Q2521/501 ,  C12Q2525/155 ,  C12Q2525/173 ,  C12Q2525/191 ,  C12Q2525/207

    Abstract: Methods, compositions and kits for capturing, detecting and quantifying mature small RNAs are provided herein. Embodiments of the methods comprise tailing both the 5' and 3' ends of mature small RNA by ligating a 5' ligation adaptor to the 5' end and polyadenylating the 3' end. Other embodiments comprise reverse transcribing the adaptor ligated, polyadenylated mature small RNA with a universal reverse transcription primer and amplifying the cDNA with universal primers.

    Abstract translation: 本文提供了用于捕获，检测和定量成熟小RNA的方法，组合物和试剂盒。 方法的实施方案包括通过将5'连接衔接子连接到5'端并将3'末端聚腺苷酸来拖尾成熟小RNA的5'和3'末端。 其他实施方案包括用通用逆转录引物逆转录连接的多聚腺苷酸成熟小RNA，并用通用引物扩增cDNA。

    METHODS, KITS & COMPOSITIONS FOR DETERMINING GENE COPY NUMBERS
    4.
    发明申请
    METHODS, KITS & COMPOSITIONS FOR DETERMINING GENE COPY NUMBERS 审中-公开
    方法，用于确定基因拷贝数的组合物

    公开(公告)号：WO2016061111A1

    公开(公告)日：2016-04-21

    申请号：PCT/US2015/055344

    申请日：2015-10-13

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： LIU, Chunmei ,  DONG, Shoulian ,  STEVENS, Junko

    IPC: C12Q1/68

    CPC classification number: C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6883 ,  C12Q2537/16 ,  C12Q2537/161 ,  C12Q2545/107 ,  C12Q2545/113 ,  C12Q2600/16

    Abstract: The present specification relates to methods, compositions, and kits for determining the presence of the number of copies of a gene. The present disclosure provides methods and compositions to determine zygosity in transgenic plants, number of transgenes in a plant or animal, karyotyping, determine CNV in human and animal samples, diagnose and detect diseases and conditions that are characterized by having variations in the number of copies of one or more genes.

    Abstract translation: 本说明书涉及用于确定基因拷贝数的存在的方法，组合物和试剂盒。 本公开提供了确定转基因植物接合度的方法和组合物，植物或动物中的转基因数量，核型分析，确定人和动物样品中的CNV，诊断和检测以拷贝数变化为特征的疾病和病症 的一个或多个基因。

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