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公开(公告)号：WO2013180813A1
公开(公告)日：2013-12-05
申请号：PCT/US2013/031637
申请日：2013-03-14
发明人： GEE, Kyle , SINGH, Upinder , RUKAVISHNIKOV, Aleksey , BEACHAM, Daniel , HUANG, Shih-Jung , JANES, Michael , ZHOU, Wenjun
CPC分类号： C09B11/24 , A61K49/0041 , C07D311/90 , G01N33/5005 , G01N33/582 , G01N33/84
摘要： Disclosed herein are compounds, compositions, methods and kits for detecting pH in samples using pH-sensitive fluorescent dyes. The compounds disclosed herein are novel xanthene-derivative dyes comprising an aniline moiety with one or more electron donating groups, which dyes are for detecting pH in samples either in vitro or in vivo. Also described herein are processes for preparing said dyes for use in the disclosed compositions, methods and kits.
摘要翻译： 本文公开了使用pH敏感荧光染料检测样品中的pH的化合物，组合物，方法和试剂盒。 本文公开的化合物是包含具有一个或多个给电子基团的苯胺部分的新型呫吨衍生物染料，该染料用于在体外或体内检测样品中的pH。 本文还描述了用于制备用于所公开的组合物，方法和试剂盒的所述染料的方法。
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2.发明申请
公开(公告)号：WO2018035230A1
公开(公告)日：2018-02-22
申请号：PCT/US2017/047152
申请日：2017-08-16
IPC分类号： G01N33/68 , C07D493/10
摘要： Compositions and methods for detecting the activity of an ion channel in a cell are described. The methods include providing a loading buffer solution to the cell, where the loading buffer includes a thallium ion indicator and optionally chloride ions, and providing a stimulus buffer that includes thallium ions to the cell. Providing the stimulus buffer can cause thallium ion influx into or efflux out of the cell through the ion channel. After providing the stimulus buffer, a change in at least one optical property of the thallium ion indicator is detected in response to thallium influx or efflux, thereby detecting the activity of the ion channel.
摘要翻译： 描述了用于检测细胞中离子通道活性的组合物和方法。 该方法包括向细胞提供上样缓冲溶液，其中上样缓冲液包含铊离子指示剂和任选的氯离子，并向细胞提供包含铊离子的刺激缓冲液。 提供刺激缓冲液可引起铊离子通过离子通道流入或流出细胞。 在提供刺激缓冲液后，铊离子指示剂的至少一种光学性质的变化响应于铊流入或流出而被检测到，从而检测到离子通道的活性。
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公开(公告)号：WO2013180811A1
公开(公告)日：2013-12-05
申请号：PCT/US2013/031535
申请日：2013-03-14
发明人： GEE, Kyle , SINGH, Upinder , RUKAVISHNIKOV, Aleksey , BEACHAM, Daniel , HUANG, Shih-Jung , JANES, Michael , ZHOU, Wenjun , BERLIER, Judith
CPC分类号： C09B11/24 , A61K49/0041 , C07D311/90 , G01N33/5005 , G01N33/582 , G01N33/84
摘要： Disclosed herein are compounds, compositions, methods and kits for detecting pH in samples using pH-sensitive fluorescent dyes. The compounds disclosed herein are novel xanthene-derivative dyes comprising an aniline moiety with one or more electron donating groups, which dyes are for detecting pH in samples either in vitro or in vivo. Also described herein are processes for preparing said dyes for use in the disclosed compositions, methods and kits.
摘要翻译： 本文公开了使用pH敏感荧光染料检测样品中的pH的化合物，组合物，方法和试剂盒。 本文公开的化合物是包含具有一个或多个给电子基团的苯胺部分的新型呫吨衍生物染料，该染料用于在体外或体内检测样品中的pH。 本文还描述了用于制备用于所公开的组合物，方法和试剂盒的所述染料的方法。
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公开(公告)号：WO2020033681A3
公开(公告)日：2020-02-13
申请号：PCT/US2019/045697
申请日：2019-08-08
发明人： YANG, Jongtae , WANG, Xin , HU, Yi-Zhen , GEE, Kyle , CHEN, Aimei , BENSON, Scott , EVANS, Brian , RUKAVISHNIKOV, Aleksey
IPC分类号： C09B69/00 , C09B11/14 , G01N33/58 , C12Q1/6869
摘要： Silicon-substituted rhodamine compounds are disclosed herein. Also described herein are Si R dyes comprising at least one vinyl groupattached to the Si atom (10 position) of the SiR dye. Derivatives, functionalized versions, conjugates, kits, related synthetic methods and uses of Si R compounds also are provided. Silicon-rhodamine (SiR) dyes can provide bright fluorescence at far red wavelengths and exhibit good photostability. The compounds described herein can be useful for fluorescent labeling and detection of biological samples. The compounds have the general structure (I) below.
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公开(公告)号：WO2020033681A2
公开(公告)日：2020-02-13
申请号：PCT/US2019/045697
申请日：2019-08-08
发明人： YANG, Jongtae , WANG, Xin , HU, Yi-Zhen , GEE, Kyle , CHEN, Aimei , BENSON, Scott , EVANS, Brian , RUKAVISHNIKOV, Aleksey
IPC分类号： C09B69/00 , C09B11/14 , G01N33/58 , C12Q1/6869
摘要： Silicon-substituted rhodamine compounds are disclosed herein. Also described herein are SiR dyes comprising at least one vinyl group attached to the Si atom (10 position) of the SiR dye. Derivatives, functionalized versions, conjugates, kits, related synthetic methods and uses of SiR compounds also are provided. Silicon-rhodamine (SiR) dyes can provide bright fluorescence at far red wavelengths and exhibit good photostability. The compounds described herein can be useful for fluorescent labeling and detection of biological samples.
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公开(公告)号：WO2018045278A1
公开(公告)日：2018-03-08
申请号：PCT/US2017/049838
申请日：2017-09-01
发明人： DESAI, Surbhi , NLEND, Marie , GEE, Kyle , BAKER, Matthew , AGGELER, Robert , SWEENEY, Scott , RUKAVISHNIKOV, Aleksey , HUANG, Shih-Jung
IPC分类号： G01N33/58 , G01N33/533
摘要： This disclosure relates to the field of fluorescent dyes, and in particular, compositions and methods for increasing fluorescent signals and the reduction of fluorescent quenching.
摘要翻译： 本公开涉及荧光染料领域，并且具体地涉及用于增加荧光信号和减少荧光猝灭的组合物和方法。
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