公开(公告)号：WO2006019388A3

公开(公告)日：2006-02-23

申请号：PCT/US2004/039762

申请日：2004-12-13

Applicant: LUMIGEN, INC. ,  AKHAVAN-TAFTI, Hashem ,  DESILVA, Renuka ,  EICKHOLT, Robert, A. ,  GUNDLACH, William, C. ,  HANDLEY, Richard, S. ,  LAUWERS, Kenneth, S. ,  SANDISON, Mark, D. ,  XIE, Wenhua

Inventor： AKHAVAN-TAFTI, Hashem ,  DESILVA, Renuka ,  EICKHOLT, Robert, A. ,  GUNDLACH, William, C. ,  HANDLEY, Richard, S. ,  LAUWERS, Kenneth, S. ,  SANDISON, Mark, D. ,  XIE, Wenhua

IPC: C12Q1/68 ,  C07H21/00 ,  C07H21/04

Abstract: Solid phase materials for binding nucleic acids and methods of their use are disclosed. The materials feature a cleavable linker portion which can be cleaved to release bound nucleic acids. The solid phase materials comprise a solid support portion comprising a matrix selected from silica, glass, insoluble synthetic polymers, and insoluble polysaccharides to which is attached a nucleic acid binding portion for attracting and binding nucleic acids, the nucleic acid binding portion (NAB) being linked by a cleavable linker portion to the solid support portion. Preferred nucleic acid binding portions comprise a ternary or quaternary onium group. The materials can be in the form of microparticles, fibers, beads, membranes, test tubes or micowells and can further comprise a magnetic core portion. Methods of binding nucleic acids using the cleavable solid supports are disclosed as are their use in methods of isolating or purifying nucleic acids.

公开(公告)号：WO2003104447A1

公开(公告)日：2003-12-18

申请号：PCT/US2003/013410

申请日：2003-05-15

Applicant: LUMIGEN, INC.

Inventor： AKHAVAN-TAFTI, Hashem ,  DE SILVA, Renuka ,  SANDISON, Mark, D. ,  HANDLEY, Richard, S.

IPC: C12N9/96

CPC classification number: C12Q1/28 ,  G01N33/533 ,  Y10S435/968

Abstract: Methods of producing fluorescence from fluorogenic substrates reactive with a peroxidase enzyme are disclosed. Use of the methods in assays for peroxidase enzymes, peroxidase-labeled analytes are provided. Fluorogenic compounds, compositions and kits for reaction with peroxidase enzymes are described. Two modes of producing fluorescent compounds are described.

Abstract translation: 公开了从与过氧化物酶反应的荧光底物产生荧光的方法。 提供了在过氧化物酶，过氧化物酶标记的分析物的测定中使用这些方法。 描述了与过氧化物酶反应的荧光化合物，组合物和试剂盒。 描述了产生荧光化合物的两种模式。

公开(公告)号：WO2003027007A1

公开(公告)日：2003-04-03

申请号：PCT/US2002/021859

申请日：2002-08-23

Applicant: LUMIGEN, INC.

Inventor： AKHAVAN-TAFTI, Hashem ,  HANDLEY, Richard, S. ,  SANDISON, Mark, D. ,  LARKIN, Randall, K.

IPC: C01B13/00

CPC classification number: C01B13/02 ,  C08F8/18 ,  C08F8/40 ,  C08F8/26 ,  C08F212/08 ,  C08F212/14 ,  C08F212/36

Abstract: Improved polymer-immobilized photosensitizer are disclosed as well as methods of preparing and using them. The polymer-immobilized photosensitizers comprise a cross-linked polymer backbone, a plurality of cationic ammonium or phosphonium groups covalently bound to the polymer backbone and an immobilized photosensitizer. The average total number of carbon atoms in the ammonium or phosphonium group is at least four and preferably at least 12. The photosensitizer can be either covalently or ionically bound to the polymer. Polymer-supported photosensitizers of the invention are unexpectedly superior in catalyzing the photosensitized oxidation of compounds containing carbon-carbon double bonds.

Abstract translation: 公开了改进的聚合物固定化光敏剂以及制备和使用它们的方法。 聚合物固定的光敏剂包括交联的聚合物主链，共价结合到聚合物主链上的多个阳离子铵或鏻基团和固定的光敏剂。 铵或鏻基团中的平均总碳原子数为至少四个，优选至少12.光敏剂可以共价或离子地结合到聚合物上。 本发明的聚合物负载的光敏剂在催化含有碳 - 碳双键的化合物的光敏化氧化方面出人意料地优异。

公开(公告)号：WO2003027004A2

公开(公告)日：2003-04-03

申请号：PCT/US2002/025946

申请日：2002-08-27

Applicant: LUMIGEN, INC.

Inventor： AKHAVAN-TAFTI, Hashem ,  HANDLEY, Richard, S. ,  SANDISON, Mark, D. ,  LARKIN, Randall, K.

IPC: C01B

CPC classification number: C08F8/44 ,  B01J31/0234 ,  B01J31/06 ,  B01J35/004 ,  C08F8/18 ,  C08F8/40 ,  C08F212/08

Abstract: Improved polymer-immobilized photosensitizer are disclosed as well as methods of preparing and using them. The polymer-immobilized photosensitizers comprise a cross-linked polymer backbone, a plurality of cationic ammonium or phosphonium groups covalently bound to the polymer backbone and an immobilized photosensitizer. The average total number of carbon atoms in the ammonium or phosphonium group is at least four and preferably at least 12. The photosensitizer can be either covalently or ionically bound to the polymer. Polymer-supported photosensitizers of the invention are unexpectedly superior in catalyzing the photosensitized oxidation of compounds containing carbon-carbon double bonds.

Abstract translation: 公开了改进的聚合物固定化光敏剂以及制备和使用它们的方法。 聚合物固定的光敏剂包括交联的聚合物主链，共价结合到聚合物主链上的多个阳离子铵或鏻基团和固定的光敏剂。 铵或鏻基团中的平均总碳原子数为至少四个，优选至少12.光敏剂可以共价或离子地结合到聚合物上。 本发明的聚合物负载的光敏剂在催化含有碳 - 碳双键的化合物的光敏化氧化方面出人意料地优异。