公开(公告)号：WO2023059952A1

公开(公告)日：2023-04-13

申请号：PCT/US2022/074568

申请日：2022-08-05

申请人： MASSACHUSETTS INSTITUTE OF TECHNOLOGY

发明人： CATANIA, Chelsea ,  FURST, Ariel ,  FAN, Gang ,  WASUWANICH, Pris

IPC分类号： A61K9/54 ,  A61K35/742 ,  C12N1/20 ,  A23L33/135 ,  A61K47/52 ,  A61K47/6903 ,  A61K9/0053 ,  C12N1/00 ,  C12N1/04 ,  C12N3/00

摘要： Prokaryotic cells having a metal-phenolic network coating are disclosed, as are compositions including such cells, methods for their use, and methods for producing such cells.

公开(公告)号：WO2023044345A1

公开(公告)日：2023-03-23

申请号：PCT/US2022/076432

申请日：2022-09-14

申请人： MASSACHUSETTS INSTITUTE OF TECHNOLOGY

发明人： FURST, Ariel ,  AHLMARK, Rachel ,  KARBELKAR, Amruta ,  REYNOLDS, Erin

IPC分类号： A62D3/02 ,  C12N1/20 ,  C12N9/16 ,  C12N15/62

摘要： Provided herein are recombinant microbial cells displaying on their surface a non-native protein capable of degrading an organophosphate, wherein the recombinant microbial cell has inhibited replication, as well as recombinant microbial cells engineered to be capable of expressing a non-native transcription factor that activates a non-native promoter in response to an organophosphate degradation product, wherein the non-native promoter is operatively linked to a nucleic acid encoding a reporter protein, wherein activity of the reporter protein can be detected, and their use for degrading organophosphates and detecting organophosphate degradation products.