公开(公告)号：WO2023006176A1

公开(公告)日：2023-02-02

申请号：PCT/EP2021/070852

申请日：2021-07-26

申请人： MAX-PLANCK-GESELLSCHAFT ZUR FÖRDERUNG DER WISSENSCHAFTEN E.V.

发明人： LEUTENEGGER, Marcel ,  WEBER, Michael ,  VON DER EMDE, Henrik ,  HELL, Stefan W.

IPC分类号： G01N21/64 ,  G02B21/16 ,  G02B21/00

摘要： For determining a molecule position of a singularized fluorophore molecule, a light beam including fluorescence excitation light and fluorescence influencing light is shaped and focused such as to form a light intensity distribution having a central intensity minimum of the fluorescence influencing light. The central intensity minimum is continuously moved along a track (31) repeatedly extending around an estimated position (34). Individual photons of fluorescence light emitted by the fluorophore molecule due to excitation by the fluorescence excitation light are registered; and an intensity minimum position of the intensity minimum is recorded for the excitation of each individual photon registered. In response, the estimated position (34) around which the track extends is updated on basis of the recorded intensity minimum position, and extensions of the track (31) around the estimated position (34) are reduced and an effectiveness of the fluorescence influencing light is increased.