公开(公告)号：WO2017124100A1

公开(公告)日：2017-07-20

申请号：PCT/US2017/013782

申请日：2017-01-17

Applicant: MEMPHIS MEATS, INC.

Inventor： GENOVESE, Nicholas ,  DESMET, Danielle Nicole ,  SCHULZE, Eric

IPC: A23L1/31 ,  C12N5/077 ,  C12N15/85

CPC classification number: C12N9/1241 ,  C12N5/0018 ,  C12N2510/00

Abstract: A product and process for extending the replicative capacity of metazoan somatic cells using targeted genetic amendments to abrogate inhibition of cell-cycle progression during replicative senescence and derive clonal cell lines for scalable applications and industrial production of metazoan cell biomass. An insertion or deletion mutation using guide RNAs targeting the first exon of the transcript encoding each protein is created using CRISPR/Cas9. Targeted amendments result in inactivation of p15 and p16 proteins which increases the proliferative capacity of the modified cell populations relative to their unaltered parental populations. Combining these amendments with ancillary telomerase activity from a genetic construct directing expression of a telomerase protein homolog from a TERT gene, increases the replicative capacity of the modified cell populations indefinitely. One application is to manufacture skeletal muscle for dietary consumption using cells from the poultry species Gallus gallus ; another is from the livestock species Bos taurus.

Abstract translation: 使用靶向遗传修饰来延长复制性衰老期间细胞周期进程的抑制并衍生克隆细胞系以用于可扩展应用和工业生产后生动物细胞的用于延长后生动物体细胞的复制能力的产品和方法 生物质。 使用靶向编码每种蛋白质的转录物的第一外显子的引导RNA的插入或缺失突变使用CRISPR / Cas9产生。 靶向修饰导致p15和p16蛋白失活，这增加了修饰的细胞群相对于其未改变的亲本群体的增殖能力。 将这些修饰与来自指导来自TERT基因的端粒酶蛋白质同源物表达的基因构建物的辅助端粒末端转移酶活性组合，可无限增加修饰的细胞群的复制能力。 一种应用是使用来自家禽物种Gallus gallus的细胞来制造用于饮食消耗的骨骼肌肉; 另一个来自家畜物种金牛座。