公开(公告)号：WO2018041828A1

公开(公告)日：2018-03-08

申请号：PCT/EP2017/071658

申请日：2017-08-29

Applicant: MIACOM DIAGNOSTICS GMBH

Inventor： VON STEIN, Walter ,  STANGE, Mirko

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q1/6841 ,  C12Q2565/107 ,  C12Q2525/301 ,  C12Q2565/1015

Abstract: A method for detecting at least one specific microorganism in a sample is disclosed,wherein a probe nucleic acid sequence comprising at least one detectable label which is capable of emitting at least one detectable signal is provided, and wherein the detectable signal takes a first value when the probe sequence is not bound to the target sequence and a second value when the probe sequence is bound to the target sequence. At least one value of the detectable signal for the probe nucleic acid sequence is measured and analyzed, wherein it is indicated that the sample contains the microorganism if the measured value corresponds to the second value of the detectable signal. A significant difference between a probe sequence that hybridizes with a target sequence (signal (2)) and a control sequence which does not bind to any target (signal (1)) can be detected. Throughout all phases (heating, stationary and cooling)of a hybridization process, the value of the detectable signal (2) is higher than the value of the control signal (1), wherein the most significant difference can be observed at the end of the cooling phase (Δ3) so that measuring and analyzing the detectable signal (2) during this period provides the most reliable result. Alternatively or optionally, additional measuring points in other phases (Δ1 and/or Δ2) can be set in order to enhance reliability of the result.

Abstract translation: 公开了用于检测样品中至少一种特定微生物的方法，其中提供包含至少一种能够发出至少一种可检测信号的可检测标记的探针核酸序列，并且其中 当探针序列未与目标序列结合时，可检测信号取第一个值，当探针序列与目标序列结合时，第二个值取第二个值。 测量和分析探针核酸序列的可检测信号的至少一个值，其中如果测量值对应于可检测信号的第二值，则表明样品含有微生物。 可以检测与靶序列（信号（2））杂交的探针序列与不与任何靶标结合的控制序列（信号（1））之间的显着差异。 在杂交过程的所有阶段（加热，静止和冷却）中，可检测信号（2）的值高于控制信号（1）的值，其中最显着的差异可以在 冷却阶段（Δ3），以便在此期间测量和分析可检测信号（2）提供最可靠的结果。 替代地或可选地，可以设置其他阶段的附加测量点（Δ1和/或Δ2）以提高结果的可靠性。