公开(公告)号：WO2012126882A1

公开(公告)日：2012-09-27

申请号：PCT/EP2012/054803

申请日：2012-03-19

Applicant: MIACOM DIAGNOSTICS GMBH ,  STEIN, FREIHERR VON, Walter ,  STANGE, Mirko

Inventor： STEIN, FREIHERR VON, Walter ,  STANGE, Mirko

IPC: C12Q1/18 ,  G01N33/569 ,  C12Q1/68 ,  C12N15/82

CPC classification number: C12Q1/689 ,  C07K16/40 ,  C12Q2600/106 ,  C12Q2600/136 ,  G01N33/569 ,  G01N2333/31 ,  G01N2800/44

Abstract: The invention relates to a method and a kit for the detection of an antibiotic resistance in a predetermined micro-organism in a biological sample. The method according to the invention comprises the following steps: (a) contacting the biological sample with a first nucleic acid labelled with a first label and capable of selectively hybridizing with a nucleic acid in the micro-organism, (b) identifying the micro-organism by the detection of the presence of the first label in an individual cell of the micro-organism, (c) contacting the sample with at least one probe for detection of an antibiotic resistance in a micro-organism, wherein said at least one probe is labelled with at least a second label, and (d) determining the antibiotic resistance of the micro-organism by the detection of the presence of at least the second label, wherein steps (b) and (d) are performed simultaneously so as to allow quick identification of a pathogen directly from a sample without culturing and without prior amplification.

Abstract translation: 本发明涉及用于检测生物样品中预定微生物中的抗生素抗性的方法和试剂盒。 根据本发明的方法包括以下步骤：（a）使生物样品与标记有第一标记并能够与微生物中的核酸选择性杂交的第一核酸接触，（b）鉴定微生物， 通过检测微生物的单个细胞中第一标记的存在，（c）使样品与至少一个用于检测微生物中的抗生素抗性的探针接触，其中所述至少一个探针 标记有至少第二标记，和（d）通过检测至少第二标记的存在来确定微生物的抗生素抗性，其中步骤（b）和（d）同时进行，以便 允许直接从样品中快速鉴定病原体而不进行培养，无需先前的扩增。

公开(公告)号：WO2012035076A1

公开(公告)日：2012-03-22

申请号：PCT/EP2011/065963

申请日：2011-09-14

Applicant: MIACOM DIAGNOSTICS GMBH ,  THRIPPLETON, Ian

Inventor： THRIPPLETON, Ian

IPC: C12Q1/68

CPC classification number: C12Q1/6841 ,  C12Q1/6806 ,  C12Q1/6832 ,  C12Q2527/125 ,  C12Q2527/153

Abstract: The invention concerns a composition comprising TCEP, biotin and dextran suitable for liquefying a viscous biological sample. The composition according to the invention can be used in diagnostic methods, preferably for use in diagnosis of an infection with a micro-organism, more preferably for use in diagnosis of HCAP.

Abstract translation: 本发明涉及一种包含TCEP，生物素和适用于液化粘性生物样品的葡聚糖的组合物。 根据本发明的组合物可用于诊断方法，优选用于诊断微生物感染，更优选用于诊断HCAP。

公开(公告)号：WO2018041828A1

公开(公告)日：2018-03-08

申请号：PCT/EP2017/071658

申请日：2017-08-29

Applicant: MIACOM DIAGNOSTICS GMBH

Inventor： VON STEIN, Walter ,  STANGE, Mirko

IPC: C12Q1/68

CPC classification number: C12Q1/689 ,  C12Q1/6841 ,  C12Q2565/107 ,  C12Q2525/301 ,  C12Q2565/1015

Abstract: A method for detecting at least one specific microorganism in a sample is disclosed,wherein a probe nucleic acid sequence comprising at least one detectable label which is capable of emitting at least one detectable signal is provided, and wherein the detectable signal takes a first value when the probe sequence is not bound to the target sequence and a second value when the probe sequence is bound to the target sequence. At least one value of the detectable signal for the probe nucleic acid sequence is measured and analyzed, wherein it is indicated that the sample contains the microorganism if the measured value corresponds to the second value of the detectable signal. A significant difference between a probe sequence that hybridizes with a target sequence (signal (2)) and a control sequence which does not bind to any target (signal (1)) can be detected. Throughout all phases (heating, stationary and cooling)of a hybridization process, the value of the detectable signal (2) is higher than the value of the control signal (1), wherein the most significant difference can be observed at the end of the cooling phase (Δ3) so that measuring and analyzing the detectable signal (2) during this period provides the most reliable result. Alternatively or optionally, additional measuring points in other phases (Δ1 and/or Δ2) can be set in order to enhance reliability of the result.

Abstract translation: 公开了用于检测样品中至少一种特定微生物的方法，其中提供包含至少一种能够发出至少一种可检测信号的可检测标记的探针核酸序列，并且其中 当探针序列未与目标序列结合时，可检测信号取第一个值，当探针序列与目标序列结合时，第二个值取第二个值。 测量和分析探针核酸序列的可检测信号的至少一个值，其中如果测量值对应于可检测信号的第二值，则表明样品含有微生物。 可以检测与靶序列（信号（2））杂交的探针序列与不与任何靶标结合的控制序列（信号（1））之间的显着差异。 在杂交过程的所有阶段（加热，静止和冷却）中，可检测信号（2）的值高于控制信号（1）的值，其中最显着的差异可以在 冷却阶段（Δ3），以便在此期间测量和分析可检测信号（2）提供最可靠的结果。 替代地或可选地，可以设置其他阶段的附加测量点（Δ1和/或Δ2）以提高结果的可靠性。

公开(公告)号：WO2013017573A1

公开(公告)日：2013-02-07

申请号：PCT/EP2012/064904

申请日：2012-07-30

Applicant: MIACOM DIAGNOSTICS GMBH ,  STANGE, Mirko ,  STEIN, FREIHERR VON, Walter

Inventor： STANGE, Mirko ,  STEIN, FREIHERR VON, Walter

IPC: C12Q1/68 ,  C12Q1/18

CPC classification number: C12Q1/18 ,  C12Q1/34 ,  G01N2333/31 ,  G01N2333/936 ,  G01N2415/00

Abstract: The invention relates to a method for detecting a resistance of at least one cell of a microorganism to at least one antibiotic substance. More specifically, the invention concerns a method for detection of resistance of pathogen bacteria to β-lactam antibiotics. According to the invention a patient's probe is treated with at least one disrupting agent that at least partially damages the cell wall of the microorganism. The concentration of the disrupting agent needed to detectably damage the cell is a measure for the sensitivity of the cell to the antibiotic substance. Surprisingly, it turned out that the cell wall of resistant strains is weakened so that treatment with a disrupting agent in low concentrations results in partial cell wall destruction. Due to cell wall destruction, marker substances can easily enter the cells so that they can be detected by standard methods. This effect is not observed with strains that are sensitive to the antibiotic so that, depending on the concentration of the disrupting agent, a clear distinction between resistant and sensitive cells can be accomplished.

Abstract translation: 本发明涉及一种用于检测至少一种微生物细胞对至少一种抗生素物质的抗性的方法。 更具体地，本发明涉及用于检测病原体细菌对β-内酰胺抗生素的抗性的方法。 根据本发明，用至少一种至少部分损伤微生物细胞壁的破坏剂处理患者的探针。 可检测地损伤细胞所需的破坏剂的浓度是细胞对抗生素物质的敏感性的量度。 令人惊讶的是，证明抗性菌株的细胞壁被削弱，使得用低浓度的破坏剂处理导致部分细胞壁破坏。 由于细胞壁破坏，标记物质可以容易地进入细胞，以便通过标准方法检测。 对抗生素敏感的菌株没有观察到这种效果，因此根据破坏剂的浓度，可以实现抗性和敏感细胞之间的明确区别。

公开(公告)号：WO2011107606A1

公开(公告)日：2011-09-09

申请号：PCT/EP2011/053341

申请日：2011-03-04

Applicant: MIACOM DIAGNOSTICS GmbH ,  THRIPPLETON, Ian Peter ,  STEIN, FREIHERR VON, Walter

Inventor： THRIPPLETON, Ian Peter ,  STEIN, FREIHERR VON, Walter

IPC: C12Q1/68

CPC classification number: C12Q1/6837 ,  C12Q1/6841 ,  C12Q2537/143

Abstract: Subject of the present invention is a combination of nucleic acid molecules capable of hybridising with a target nucleic acid sequence. In order to overcome problems with the reproducibility of FISH assays and to decrease assay time, hairpin probes are used in combination with helper probes annealing adjacent to the target site of the hairpin probe.

Abstract translation: 本发明的主题是能够与靶核酸序列杂交的核酸分子的组合。 为了克服FISH测定的重现性和减少测定时间的问题，发夹探针与与发夹探针的靶位点相邻退火的辅助探针组合使用。